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| Status |
Public on Jan 23, 2018 |
| Title |
NR4A1 and NR4A3 Restrict HSC Proliferation via Reciprocal Regulation of C/EBPα and Inflammatory Signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
NR4A nuclear receptors are known tumor suppressors of acute myeloid leukemia and are essential regulators of hematopoietic stem cell (HSC) quiescence. To study the transcriptional consequences of acute NR4A1/3 codepletion in HSCs, we used an Nr4a1/3 conditional knockout mouse model and performed a global transcriptome profiling using RNA-Seq in sorted HSCs four days after NR4A1/3 codepletion.
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| |
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| Overall design |
CDKO mice (Nr4a1 fl/fl; Nr4a3-/-; Rosa26-ERt2) and control mice (Nr4a1 fl/fl; Nr4a3-/-) were treated with 4 daily tamoxifen injections (120 mg/Kg) to incduce Nr4a1 excision and hematopoietic stem cells were sorted on day 5 for RNA-Seq analysis. Each sample consists in a pool of 3-5 mice.
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| |
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| Contributor(s) |
Freire PR, Conneely OM |
| Citation(s) |
29343483 |
| |
| Submission date |
Jul 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Pablo Riera Freire |
| E-mail(s) |
freire@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA393578 |
| SRA |
SRP111411 |
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