|
| Status |
Public on Jan 31, 2018 |
| Title |
Mechanisms of the innate defense regulator peptide 1002 anti-inflammatory activity in a sterile inflammation mouse model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Innate defense regulator (IDR) peptide-1002 is a synthetic host defense peptide derivative with strong anti-inflammatory properties.To investigate the anti-inflammatory mechanisms of IDR-1002 in vivo, the phorbol 12-myristate 13-acetate (PMA)-induced mouse ear inflammation model was used. Topical IDR-1002 treatment successfully dampened PMA-induced ear edema, proinflammatory cytokine production, reactive oxygen and nitrogen species release, and neutrophil recruitment in the ears of CD1 mice. Advanced RNA-Seq analysis on the mouse ear transcriptome revealed that IDR-1002 reduced sterile inflammation by suppressing the expression of transmembrane G-protein coupled receptors (Class A/1 rhodopsin-like), including receptors for chemokines, prostaglandins, histamine, platelet activating factor and anaphylatoxin. IDR-1002 also dampened IFNγ response and repressed the Interferon regulatory factor (Irf) 8-regulated network that controls central inflammatory pathways.
|
| |
|
| Overall design |
RNA-Seq profiling of gene expression in mouse ear tissue exposed to three treatment conditions
|
| |
|
| Contributor(s) |
Wu B, Lee AH, Hancock RE |
| Citation(s) |
28993516 |
| |
| Submission date |
Jul 07, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Erin Gill |
| E-mail(s) |
erin.gill81@gmail.com
|
| Organization name |
University of British Columbia
|
| Street address |
2259 Lower Mall
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA393432 |
| SRA |
SRP111338 |
Less...
More...