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Series GSE100881 Query DataSets for GSE100881
Status Public on Jul 01, 2018
Title Analysis of RNA-seq libraries for conditional knock-out of transcription factors in adult mouse pancreas
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The purpose of this study is to determine the roles and hierarchy of the transcription factors Foxa2, Gata4, Nr5a2, and Ptf1a in the control of the physiology of the adult exocrine pancreas. Knockouts of each gene were induced by tamoxifen treatment of mice bearing a Ptf1aCreER allele as well as floxed alleles of the gene(s) to be knocked out. Four to eight RNA-seq libraries were analyzed for control animals, knockouts of each single gene, pairwise knockouts of Ptf1a & Foxa2, Ptf1a & Gata4, Ptf1a & Nr5a2, Foxa2 & Gata4, Gata4 & Nr5a2, and a four gene knockout of Ptf1a, Foxa2, Gata4, and Nr5a2 (eleven different genotypes in all).
 
Overall design Each RNA-seq library was synthesized from pancreas RNA

This series includes the re-analysis of three samples from GSE86261 (GSM2299174, GSM2299175 and GSM2299181).
 
Contributor(s) Azevedo-Pouly AC, Swift G, MacDonald R
Citation(s) 37796967
Submission date Jul 06, 2017
Last update date Oct 18, 2023
Contact name Galvin Swift
E-mail(s) galvin.swift@utsouthwestern.edu
Organization name U TX Southwestern Medical Center
Department Molecular Biology
Lab Ray MacDonald
Street address 6000 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390-9148
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (60)
GSM2695500 Ptf1a(CreER/+) 3Ptf1aFC-41-9
GSM2695501 Ptf1a(CreER/+) 3Ptf1aFC-41-11
GSM2695502 Ptf1a(CreER/+) Ptf1aC-10-3_re-analyzed
Relations
BioProject PRJNA393344
SRA SRP111204

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100881_Pancreas_TF-ko_RNAseq_Summary.xlsx 11.0 Mb (ftp)(http) XLSX
GSE100881_RAW.tar 5.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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