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| Status |
Public on Oct 31, 2017 |
| Title |
Transcriptome of specific cell types residing in human subcutaneous adipose tissue |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptome of specific cell types residing in human subcutaneous adipose tissue
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| Overall design |
White adipose tissue (WAT) from 3 lean and 3 overweight-obese patients was collagenase treated and adipocytes were separated from the stromal vascular fraction (SVF). SVF was then FACS sorted for the following fractions CD45-/CD34+/CD31- (progenitor), CD45+/CD14+/CD206+ (total macrophages), CD45+/CD14+/CD206+/CD11c+ (M1 macrophages), CD45+/CD14+/CD206+/CD11c- (M2 macrophages), CD45+/CD3+ (total T cells), CD45+/CD3+/CD4+/CD8- (Th T-cells), CD45+/CD3+/CD4-/CD8+ (Tc T-cells). RNA was isolated from all these fractions as well as from adipocytes and analyzed on the Affymetrix Human ClariomTMD microarray.
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| Contributor(s) |
Laurencikiene J, Acosta J |
| Citation(s) |
29116032 |
| Submission date |
Jul 05, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jurga Laurencikiene |
| Organization name |
Karolinska Institutet
|
| Street address |
Hälsovägen 7
|
| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
|
| Samples (32)
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| Relations |
| BioProject |
PRJNA393100 |
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