|
| Status |
Public on Oct 03, 2018 |
| Title |
Gene Expression Profiling of SPOP Knocked Down Cell |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
SPOP is one of the most frequent mutated genes in prostate cancer. In the current study, we identified CCNE1 as its substrate. SPOP directly interacts with CCNE1, poly-ubiquitinates CCNE1 in vivo and in vitro, and represses cancer-related phenotypes induced by CCNE1 expression. Thus, we reveal a new mechanism for SPOP in repressing prostate cancer tumorigenesis.
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| |
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| Overall design |
SPOP was knocked down by siRNA in DU145 and 769-P, the gene expression profile was studied by RNA sequencing
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| |
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| Contributor(s) |
Ju L, Zhu Y |
| Citation(s) |
30237511 |
| |
| Submission date |
Jul 03, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Pin-Ji Lei |
| E-mail(s) |
leipinji@gmail.com
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Department of Radiation Oncology
|
| Lab |
Edwin L. Steele Laboratories
|
| Street address |
100 Blossom Street
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA392902 |
| SRA |
SRP110985 |
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