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| Status |
Public on Feb 13, 2018 |
| Title |
RNA-Seq analysis of breast cancer cells after shikonin treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA-seq analysis using differnt breast cancer cell lines (MCF-7, SK-BR-3 and MDA-MB-231) after shikonin treatment. We reported that shikonin enhances the expression of DUSP-1 and DUSP-2. Shikonin induces apoptosis and decreases the phosphorylation of JNK1/2 through activationg DUSP-1 and DUSP2.
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| Overall design |
We treated three breast cancer cell lines with 10 μΜ shikonin for 6 hr. The gene expression profiling was performed using RNA-seq. Numbers of significantly differentially expressed (SDE) genes in different human breast cancer cell lines under shikonin treatment had been identified. We analyzed the functional annotation and performed the pathway analysis using 38 common SDE genes.
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| Contributor(s) |
Lin KH, Huang MY, Cheng WC, Wang SC, Fang SH, Liu PL, Su CC, Chen CS, Shen KH, Wang YT, Li CY |
| Citation(s) |
29422643 |
| |
| Submission date |
Jun 30, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
CHIA-YANG LI |
| E-mail(s) |
chiayangli@gmail.com
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| Phone |
+886-909117068
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| Organization name |
Kaohsiung Medical University
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| Department |
Graduate Institute of Medicine
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| Street address |
100,Shih-Chuan 1st Road
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| City |
Kaohsiung |
| ZIP/Postal code |
807 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA392672 |
| SRA |
SRP110816 |
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