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| Status |
Public on Sep 13, 2017 |
| Title |
Subset- and tissue-defined STAT5 thresholds control homeostasis and function of innate lymphoid cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Innate lymphoid cells (ILCs) patrol environmental interfaces to defend against infection and protect barrier integrity. Using a genetic tuning model, we demonstrate that the signal-dependent transcription factor STAT5 is critical for accumulation of all known ILC subsets in mice, and reveal a hierarchy of STAT5 dependency for populating lymphoid and non-lymphoid tissues. We also apply transcriptome and genomic distribution analyses to define a STAT5 gene signature in NK cells, the prototypical ILC subset, and provide a systems-based molecular rationale for its key functions downstream of IL-15. Additionally, we uncover surprising features of STAT5 behavior, most notably, the wholesale re-distribution that occurs when NK cells shift from tonic signaling to acute cytokine-driven signaling, and its genome-wide coordination with T-bet, another key transcription factor in ILC biology. Collectively, our data position STAT5 as a central node in the transcription factor network that instructs ILC development, homeostasis and function, and provide mechanistic insights on how it works at cellular and molecular levels.
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| Overall design |
This dataset includes 22 individual samples of RNAseq or STAT5 ChIPseq data from ex vivo or cytokine-treated NK cells. Each culture condition includes at least 2 biological replicates per genotype.
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| Contributor(s) |
Villarino AV, Sun HW, Gutierrez-Cruz G, Brooks SR, Kanno Y, O'Shea JJ |
| Citation(s) |
28916644 |
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| Submission date |
Jun 29, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Yuka Kanno |
| E-mail(s) |
kannoy@mail.nih.gov
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| Phone |
301-402-3008
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| Organization name |
NIH
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| Department |
NIAMS
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| Lab |
LCBS-MIIB
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| Street address |
10 Center Drive, Rm13C120
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-1930 |
| Country |
USA |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA392693 |
| SRA |
SRP110826 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100674_JEM_RPKM_table.xlsx |
3.7 Mb |
(ftp)(http) |
XLSX |
| GSE100674_RAW.tar |
276.7 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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