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| Status |
Public on Dec 26, 2019 |
| Title |
Histamine H3 receptor agonist regulates the expression of inflammation-related genes in kidney and heart of ANS-treated mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We found the impaired renal function accompanied with severe cardiac dysfunction in mice co-treated with angiotensin II, nephrectomy and salt (ANS), and identified the increased level of plasma histamine in ANS-treated mice. Interestingly, pro-inflammatory genes were increased in kidneys of ANS-treated animals, which were down-regulated by histamine H3 receptor (H3R) agonist. Purpose: To evaluate the molecular mechanisms underlying the effect of the H3R agonist on the ANS-induced renal and cardiac inflammation, we performed a comprehensive analysis of ANS-mediated gene expression changes with/without H3R agonist in kidneys and hearts using RNA sequencing (RNA-Seq). Results: To investigate differentially expressed genes (false discovery rate (FDR) p<0.05, fold change >2) among three groups, we performed pairwise comparisons of RNA-Seq data using the CLC Genomics Workbench software. In comparison with the control of kidney group, 1,283 (1,010 up- and 273 down-regulated) unique genes were significantly changed in the ANS-treated group. Meanwhile, the data set of ANS-treated kidneys with H3R agonist revealed significant changes in 234 (65 up- and 169 down-regulated) genes as compared with the control groups. In case of hearts, the ANS-treated group revealed that 85 (66 up- and 19 down-regulated) unique genes were significantly changed compared to the control groups. Furthermore, in comparison with the ANS-treated with H3R agonist group, one up-regulated gene was significantly changed in the ANS-treated group.
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| Overall design |
Renal and cardiac mRNA profiles of control (Sham), angiotensin II, nephrectomy and salt (ANS)-treated and ANS-treated with H3R agonist mice were generated by RNA sequencing using the NextSeq 500 (Illumina).
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| Web link |
https://www.pnas.org/content/early/2020/01/27/1909124117.long
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| Contributor(s) |
Kim J, Noguchi K, Fukamizu A |
| Citation(s) |
31992639 |
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| Submission date |
Jun 28, 2017 |
| Last update date |
Jan 30, 2020 |
| Contact name |
Jun-Dal Kim |
| Organization name |
University of Toyama
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| Department |
Department of Complex Biosystem Research (CBR), Institute of National Medicine (INM)
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| Lab |
Complex Biosystem Research Lab.
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| Street address |
2630 Sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA392329 |
| SRA |
SRP110715 |
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