 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 31, 2018 |
| Title |
SET-9 and SET-26, the C. elegans homologs of human MLL5, are critical for germline development and longevity |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
SET-9 and SET-26 are highly homologous paralogs that share redundant function in germline development, but SET-26 alone plays a key role in longevity and heat stress response. SET-26 is broadly expressed, but SET-9 is only detectable in the germline, which likely account for their different biological roles. SET-9 and SET-26 bind to H3K4me3 in vitro and in vivo, and their loss results in broadening of H3K4me3 domains. SET-9 and SET-26 also regulate the RNA expression of a subset of their target genes, irrespective of their H3K4me3 status. We propose that SET-9 and SET-26 function to organize local chromatin environment and to regulate the expression of specific target genes, and these activities together contribute to their critical roles in germline development, stress response, and longevity. SET-9 and SET-26 are highly homologous to human MLL5, a factor implicated in leukemia and glioblastoma, and our findings help to provide new insights into MLL5.
|
| |
|
| Overall design |
H3K4me3 in N2 and set-9 set-26; and SET-9/26 ChIP; RNA-seq for N2, set-9, set-26 and set-9 set-26
|
| |
|
| Contributor(s) |
Wang W |
| Citation(s) |
29714684 |
| |
| Submission date |
Jun 28, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Siu Sylvia Lee |
| E-mail(s) |
leelabcornell@gmail.com
|
| Organization name |
Cornell University
|
| Street address |
520 Campus road
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14850 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
| GPL19757 |
Illumina NextSeq 500 (Caenorhabditis elegans) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA392315 |
| SRA |
SRP110703 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100623_SET926GFPChIP_pooled_bedgraph_forIGFview.bedgraph.gz |
11.7 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE100623_n2set9set26set9set26rnaseq.txt.gz |
544.2 Kb |
(ftp)(http) |
TXT |
| GSE100623_set9set26H3K4me3ChIP_pooled_bedgraph_forIGFview.bedgraph.gz |
12.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE100623_wtH3K4me3ChIP_pooled_bedgraph_forIGFview.bedgraph.gz |
12.2 Mb |
(ftp)(http) |
BEDGRAPH |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...