 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 13, 2018 |
| Title |
Comparative cardiac induction time-courses using wild-type or genetically modified human embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This study compares directed cardiac differentiation time-courses using (i) HuES6 cells with endogenous ISL1 knockout + inducible ISL1 transgene, and (ii) wild-type HuES6 cells.
For the first series, a subset of samples was left untreated (= ISL1 knockout scenario) while another subset received doxycycline treatment to upregulate ISL1 at days 3 and 4 of differentiation (ISL1 rescue). For the second series, a subset of samples was differentiated under default conditions (no RA) while another subset received retinoic acid (RA) on days 3 and 4 of differentiation, which leads to an atrial-like phenotype.
|
| |
|
| Overall design |
Total RNA was isolated in a time course manner from the distinct series of differentiating samples (on days 0, 1, 2, 3, 4, 5, 6, and 8). Note that the two subsets of each series are only diverging from day 4 onwards.
|
| |
|
| Contributor(s) |
Quaranta R, Greber B |
| Citation(s) |
29337667 |
| |
| Submission date |
Jun 28, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Boris Greber |
| E-mail(s) |
b.greber@rheincell.de
|
| Phone |
+49 251 8346906
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Lab |
Human Stem Cell Pluripotency
|
| Street address |
Von-Esmarch-Str. 54
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (24)
|
| GSM2687635 |
+/- DOX time-course, day 3 |
| GSM2687636 |
+/- DOX time-course, no-DOX series, day 4 |
| GSM2687637 |
+/- DOX time-course, DOX series, day 4 |
| GSM2687638 |
+/- DOX time-course, no-DOX series, day 5 |
| GSM2687639 |
+/- DOX time-course, DOX series, day 5 |
| GSM2687640 |
+/- DOX time-course, no-DOX series, day 6 |
| GSM2687641 |
+/- DOX time-course, DOX series, day 6 |
| GSM2687642 |
+/- DOX time-course, no-DOX series, day 8 |
| GSM2687643 |
+/- DOX time-course, DOX series, day 8 |
| GSM2687644 |
+/- RA time-course, day 0 |
| GSM2687645 |
+/- RA time-course, day 1 |
| GSM2687646 |
+/- RA time-course, day 2 |
| GSM2687647 |
+/- RA time-course, day 3 |
| GSM2687648 |
+/- RA time-course, no-RA series, day 4 |
| GSM2687649 |
+/- RA time-course, no-RA series, day 5 |
| GSM2687650 |
+/- RA time-course, no-RA series, day 6 |
| GSM2687651 |
+/- RA time-course, no-RA series, day 8 |
| GSM2687652 |
+/- RA time-course, RA series, day 4 |
| GSM2687653 |
+/- RA time-course, RA series, day 5 |
| GSM2687654 |
+/- RA time-course, RA series, day 6 |
| GSM2687655 |
+/- RA time-course, RA series, day 8 |
|
| Relations |
| BioProject |
PRJNA392243 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100592_Non-normalized_data.txt.gz |
6.9 Mb |
(ftp)(http) |
TXT |
| GSE100592_RAW.tar |
64.6 Mb |
(http)(custom) |
TAR (of IDAT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...