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| Status |
Public on Apr 10, 2020 |
| Title |
A critical role of histone deacetylases, Mbd3/NuRD and Tet2 in epithelial-mesenchymal cell plasticity and in tumor invasion and metastasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have generated and employed reversible and irreversible EMT models of murine breast cancer cells to identify the key players establishing cell state transitions during a reversible and an irreversible EMT. We demonstrate that the Mbd3/NuRD complex, involving histone deacetylases (HDACs) and Tet2 hydroxylase, acts as an epigenetic block in epithelial-mesenchymal plasticity. These epigenetic modifiers keep breast cancer cells in a stable mesenchymal state, and their pharmacological inhibition or genetic ablation leads to a mesenchymal-epithelial transition (MET) and represses primary tumor growth and metastasis formation of highly aggressive, mesenchymal breast cancer cells.
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| Overall design |
We performed RNA-sequencing of 2 replicates of each treatment and cell line
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| Contributor(s) |
Kilinc A, Kalathur RR, Christofori G |
| Citation(s) |
31666683 |
| |
| Submission date |
Jun 27, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Ravi Kiran Reddy KALATHUR |
| E-mail(s) |
ravikiran.k@gmail.com
|
| Organization name |
University of Basel
|
| Department |
Departement Biomedizin
|
| Street address |
Mattenstrasse 28
|
| City |
Basel |
| State/province |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA392104 |
| SRA |
SRP110598 |
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