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| Status |
Public on Jun 27, 2017 |
| Title |
Effect of O-GlcNAc transferase deficiency on intestinal epithelial cells in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
O-GlcNAcylation is the modification of serine and threonine residues with beta-N-acetylglucosamine (O-GlcNAc) on intracellular proteins. To investigate the role of protein O-GlcNAcylation on intestinal homeostasis, we generated intestinal epithelial cell (IEC)-specific O-GlcNAc transferase (OGT) knockout in mice. The KO mice developed spontanous intestinal inflammation. To determine the underlying molecular mechanisms, we performed RNA sequencing of ileum and colon epithelial cells of wildtype and IEC-OGT KO mice.
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| |
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| Overall design |
mRNA profiling of intestinal epithelial cells (IECs) from the ileum and colon of 10-week-old wildtype and IEC-OGT KO male mice.
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| |
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| Contributor(s) |
Ruan H |
| Citation(s) |
29941542 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R21 AI140109 |
O-GlcNAc signaling enables the intestinal epithelial remodeling associated with type 2 immunity |
UNIVERSITY OF MINNESOTA |
Hai-Bin Ruan |
|
| |
| Submission date |
Jun 26, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Hai-Bin Ruan |
| E-mail(s) |
hruan@umn.edu
|
| Phone |
6123017686
|
| Organization name |
University of Minnesota
|
| Department |
Integrative Biology and Physiology
|
| Street address |
2231 6th St SE, 3-143 CCRB
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (11)
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| Relations |
| BioProject |
PRJNA391919 |
| SRA |
SRP110508 |
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