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| Status |
Public on Sep 05, 2018 |
| Title |
Minipuberty and Thymic Sexual Dimorphism in the Infant Human Thymus [puberty miRNA] |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
AIRE expression in thymus is downregulated by estrogen after puberty, what probably renders women more susceptible to autoimmune disorders. Here we investigated the effects of minipuberty on male and female infant human thymic tissue in order to verify if this initial transient increase in sex hormones - along the first six months of life - could affect thymic transcriptional network regulation and AIRE expression. Gene co-expression network analysis for differentially expressed genes and miRNA-target analysis revealed sex differences in thymic tissue during minipuberty, but such sex differences were not detected in thymic tissues from donors aged 7-18 moths of age, the non-puberty group. AIRE expression was essentially the same in both sexes in minipuberty and in non-puberty groups, as assessed by genomic and immunohistochemical assays. However, AIRE-interactors networks showed several differences in all groups regarding gene-gene expression correlation. Therefore, minipuberty and genomic mechanisms interact in shaping thymic sexual dimorphism along the first six moths of life.
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| Overall design |
Thymus miRNA transcriptomic profiles of patients undergoing minipuberty (aged up to 6 months) comparing male versus female infants in order to identify differentially expressed transcripts.
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| Contributor(s) |
Moreira-Filho CA, Bando SY, Bertonha FB, Ferreira LR, Vinhas CF, Oliveira LH, Zerbini MC, Furlanetto G, Chacur P, Coutinho A, Carneiro-Sampaio M |
| Citation(s) |
30177771 |
| |
| Submission date |
Jun 19, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Carlos Alberto Moreira-Filho |
| E-mail(s) |
carlos.moreira@hc.fm.usp.br
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| Phone |
55-11-3061-8449
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| Organization name |
Faculdade de Medicina da Universidade de Sao Paulo
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| Department |
Departments of Pediatrics
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| Lab |
LIM36
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| Street address |
Av. Dr. Eneas Carvalho de Aguiar 647
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| City |
Sao Paulo |
| State/province |
SP |
| ZIP/Postal code |
05403-900 |
| Country |
Brazil |
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| Platforms (1) |
| GPL21576 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (Probe Name version) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE113597 |
Minipuberty and Sexual Dimorphism in the Infant Human Thymus |
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| Relations |
| BioProject |
PRJNA390983 |
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