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| Status |
Public on May 28, 2008 |
| Title |
Identification of RGA downstream genes by using steroid-inducible system |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
To understand the transcriptional program controlled by RGA, we took advantage of a functional steroid-inducible RGA proteins system in combination with microarray analysis to identify RGA target genes.
Keywords: steroid-inducible system
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| Overall design |
We firstly compared the transcriptomes in ga1-3rgargl2RGA-GR inflorescence without flowers later than stage 12 induced by dexamesone- relative to mock-treatment, which revealed the differentially expressed genes corresponding to the RGA protein activity induced by dexamesone.As negative controls to exclude unexpected effects of transgenic T-DNA and Dex itself, RNAs derived from nontransgenic plants (ga1-3 rga rgl2) inflorescences treated with Dex were used. Three sets of biological independent replicates were collected.
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| |
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| Citation(s) |
18502975 |
| |
| Submission date |
Dec 24, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Hao Yu |
| E-mail(s) |
dbsyuhao@nus.edu.sg
|
| Phone |
65-65163048
|
| Fax |
65-67792486
|
| Organization name |
National University of Singapore
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| Department |
Biological Sciences
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| Lab |
Plant Functional Genomics Lab
|
| Street address |
Lower Kent Ridge Road
|
| City |
Singapore |
| ZIP/Postal code |
117543 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA108209 |
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