|
| Status |
Public on May 31, 2018 |
| Title |
A data mining paradigm for identifying key factors in biological processes using gene expression data |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-Seq was performed on the mouse keratinocyte cultures with Sbsn knockeddown to study the role of Sbsn in the regulation of epidermis devleopment.
|
| |
|
| Overall design |
The RNA-Seq was performed on mouse keratinocyte cultures with three treatments (no siRNA treated, siControl treated and siSbsn treated) of high calcium concentration. Two batches of replicates were generated for each condition. batch 1 has two replicates for each of the three conditions, and batch2 has three replicates for two conditions (siControl and siSbsn). The comparison between siSbsn vs siControl was performed to identify the differentially expressed genes.
|
| |
|
| Contributor(s) |
Li J, Zheng L, Uchiyama A, Bin L, Mauro TM, Elias PM, Pawelczyk T, Sakowicz-Burkiewicz M, Trzeciak M, Leung DY, Morasso MI, Yu P |
| Citation(s) |
29899432 |
| |
| Submission date |
Jun 16, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Maria I. Morasso |
| E-mail(s) |
morassom@mail.nih.gov
|
| Phone |
301-402-2888
|
| Organization name |
National Institutes of Health
|
| Department |
National Institute for Arthritis and Musculoskeletal and Skin Diseases
|
| Lab |
Laboratory of Skin Biology
|
| Street address |
Building 50, Room 1525 10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20814 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA390718 |
| SRA |
SRP109348 |
Less...
More...