GEO help: Mouse over screen elements for information.
Status
Public on Oct 01, 2010
Title
HAC Human 2.3K
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
Hospital A.C. Camargo
Manufacture protocol
Glass arrays with 2,352 elements were prepared with the aid of the Flexis Robot (Genomic Solutions, UK). Bacteria were grown in LB medium containing ampicillin (250mg/ml). Inserts were amplified by PCR using M13 reverse and forward primers and products were submitted to 1% agarose gel for quality control. cDNA clones with single amplicons were filtrated in Sephadex G50 (GE Healthcare, UK), printed onto Corning glass slides (Corning, USA) and fixed by UV crosslink (Eisen & Brown, 1999; Brentani et al., 2005). There were 1364 unique genes in the array. Those genes were defined trough literature search for genes related to
Contributor(s)
Carraro DM , Brentani H , Fiorine A , Palha J Submission date
Nov 17, 2009
Last update date
Oct 01, 2010
Contact name
Renato David Puga
Organization name
Universidade de São Paulo
Department
Instituto de Psiquiatria
Lab
Laboratório de Genética
Street address
Dr Ovidio Pires de Campos,785
City
São Paulo
State/province
São Paulo
ZIP/Postal code
05403-010
Country
Brazil
Samples (169)
GSM471833 , GSM471834 , GSM471835 , GSM471836 , GSM471837 , GSM471838
GSM471839 ,
GSM471840 ,
GSM471841 ,
GSM471842 ,
GSM471843 ,
GSM471844 ,
GSM471845 ,
GSM471846 ,
GSM471847 ,
GSM471848 ,
GSM471849 ,
GSM471850 ,
GSM471851 ,
GSM471852 ,
GSM471853 ,
GSM471854 ,
GSM471855 ,
GSM471856 ,
GSM471857 ,
GSM471858 ,
GSM471859 ,
GSM471860 ,
GSM471861 ,
GSM471862 ,
GSM471863 ,
GSM471864 ,
GSM471865 ,
GSM471866 ,
GSM471867 ,
GSM471868 ,
GSM471869 ,
GSM471870 ,
GSM471871 ,
GSM471872 ,
GSM471873 ,
GSM471874 ,
GSM471875 ,
GSM471876 ,
GSM471877 ,
GSM471878 ,
GSM471879 ,
GSM471880 ,
GSM471881 ,
GSM471882 ,
GSM471883 ,
GSM471884 ,
GSM471885 ,
GSM471886 ,
GSM471887 ,
GSM471888 ,
GSM471889 ,
GSM471890 ,
GSM471891 ,
GSM471892 ,
GSM471893 ,
GSM471894 ,
GSM471895 ,
GSM471896 ,
GSM471897 ,
GSM471898 ,
GSM471899 ,
GSM471900 ,
GSM471901 ,
GSM471902 ,
GSM471903 ,
GSM471904 ,
GSM471905 ,
GSM471906 ,
GSM471907 ,
GSM471908 ,
GSM635505 ,
GSM635506 ,
GSM635507 ,
GSM635508 ,
GSM635509 ,
GSM635510 ,
GSM635511 ,
GSM635512 ,
GSM635513 ,
GSM635514 ,
GSM635515 ,
GSM635516 ,
GSM635517 ,
GSM635518 ,
GSM635519 ,
GSM635520 ,
GSM635521 ,
GSM635522 ,
GSM635523 ,
GSM635524 ,
GSM635525 ,
GSM635526 ,
GSM635527 ,
GSM635528 ,
GSM635529 ,
GSM635530 ,
GSM635531 ,
GSM635532 ,
GSM635533 ,
GSM635534 ,
GSM635535 ,
GSM635536 ,
GSM635537 ,
GSM635538 ,
GSM635539 ,
GSM635540 ,
GSM635541 ,
GSM635542 ,
GSM635543 ,
GSM635544 ,
GSM635545 ,
GSM635546 ,
GSM635547 ,
GSM635548 ,
GSM635549 ,
GSM635550 ,
GSM635551 ,
GSM635552 ,
GSM635553 ,
GSM635554 ,
GSM635555 ,
GSM635556 ,
GSM635557 ,
GSM635558 ,
GSM635559 ,
GSM635560 ,
GSM635561 ,
GSM635562 ,
GSM635563 ,
GSM635564 ,
GSM635565 ,
GSM635566 ,
GSM635567 ,
GSM635568 ,
GSM635569 ,
GSM635570 ,
GSM635571 ,
GSM635572 ,
GSM635573 ,
GSM635574 ,
GSM635575 ,
GSM635576 ,
GSM635577 ,
GSM635578 ,
GSM635579 ,
GSM635580 ,
GSM635581 ,
GSM635582 ,
GSM990657 ,
GSM990658 ,
GSM990659 ,
GSM990660 ,
GSM990661 ,
GSM990662 ,
GSM990663 ,
GSM990664 ,
GSM990665 ,
GSM990666 ,
GSM990667 ,
GSM990668 ,
GSM990669 ,
GSM990670 ,
GSM990671 Series (3)
GSE19112 Using the expression profile of peripheral blood to investigate regulatory pathways in schizophrenia
GSE25965 Transduction signaling signature of Wilms tumor revealed by parallel analysis of kidney differentiation
GSE40328 Signaling pathways in skeletal muscle of Amyotrophic Lateral Sclerosis mouse model
Data table header descriptions ID gridRow Row within array
gridColumn Column within array
spotRow Row within sub-array
spotColumn Column within sub-array
SPOTID Sequence
GeneSymbol Gene symbol
SEQ_ACC Accession Number of sequence spotted
GB_ACC GenBank accession number
ORF SPOT_ID
Data table ID gridRow gridColumn spotRow spotColumn SPOTID GeneSymbol SEQ_ACC GB_ACC ORF SPOT_ID 1
1
1
1
1
CONTROL
CONTROL
--CONTROL
2
1
1
1
2
CONTROL
CONTROL
--CONTROL
3
1
1
1
3
34039
EDG3
EDG3 4
1
1
1
4
LGEA-RHSD01-230306-023-B07
FAM83D
FAM83D 5
1
1
1
5
LGEA-RHSD02-270606-026-C09
DA318042
DA318042 6
1
1
1
6
CTC_RHFADT_080705_026_F07_NM_0
BTAF1
BTAF1 7
1
1
1
7
CONTROL
CONTROL
--CONTROL
8
1
1
2
1
CONTROL
CONTROL
--CONTROL
9
1
1
2
2
CONTROL
CONTROL
--CONTROL
10
1
1
2
3
34130
NCAM2
NCAM2 11
1
1
2
4
LGEA-RHID01-080306-013-H04
JTB
JTB 12
1
1
2
5
LGEA-RHID02-260406-016-G12
AB209172
AB209172 13
1
1
2
6
CTC_RHFADT_040805_212_B03_NM_0
THRAP1
THRAP1 14
1
1
2
7
CONTROL
CONTROL
--CONTROL
15
1
1
3
1
RC3-CI0195-011-f10
GAK
GAK 16
1
1
3
2
PM3-HT0907-003-a08
COL1A2
COL1A2 17
1
1
3
3
CM0-TN0032-534-h02
SNX8
SNX8 18
1
1
3
4
CONTROL
CONTROL
--CONTROL
19
1
1
3
5
LGEA-RHID01-270606-016-D02
CSTF1
CSTF1 20
1
1
3
6
CTC_RHFADN_300605_013_B08_NM_0
FOSL1
FOSL1
2352 110 Kbytes .Supplementary data files not provided
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