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Platform GPL5374 Query DataSets for GPL5374
Status Public on Dec 17, 2007
Title NLI_SSC_11.5K_cDNA_V1
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Sus scrofa
Manufacturer China Agricultural University
Manufacture protocol Plasmid inserts were amplified by PCR using T3 (5’-GTA AAACGACGGCCAGT-3’) and T7 (5’-CAGGAAACAG CTATGAC-3’) flanking vector primers in 50ul volume reactions. Amplifications were performed in a thermal cycler (PE Amp9700, Perkin-Elmer, USA). PCR reaction mixes contained 5ul 10×Taq polymerase buffer solution, 50 pmol of each primer, 4ul 10 mM dNTPs, and 1 U Taq polymerase. An initial 5 min denaturation at 95oC was followed by 35 cycles of 1 min denaturation at 95oC, 1 min annealing at 55oC and 1 min elongation at 72oC. The reaction ended with an additional incubation of 5 min at 72oC. PCR products were analyzed on 1.0% agarose gels. Samples containing multiple bands or weak products were reamplified. PCR products were precipitated with isopropanol and redissolved in 50% dimethyl sulphoxide (DMSO). A commercial OmniGridTM microarrayer (Genomic Instrumentation Services, Inc, San Carlos, CA) was employed to print all spots on Telechem Superamine slides (Arrayit, Sunnyvale, CA) and to verify reproducibility each spot was arrayed three replicates. Control samples included the expressed pig genes encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein L24 (RPL24), eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), glutathione peroxidase 1 (GPX1), and peptidylprolyl isomerase A (cyclophilin A) (PPIA). Additional, control samples including three different Arabidopsis thaliana DNA fragments (GenBank accession numbers AC004146, AC007661 and U09332) and 12 spots of DMSO were also arrayed. The centre-to-centre distance between two adjacent spots was 225 m. After printing, the slides were baked for 1 h at 80oC and stored dry at room temperature until use.
 
 
Contributor(s) Guo W, Zhang J, Zhang WW, Deng XM, Hu XX, Li N
Submission date Jun 10, 2007
Last update date Dec 17, 2007
Contact name Jin Zhang
E-mail(s) zhangjin7688@163.com
Organization name China Agricultural University
Street address 2#, Yuanmingyuan West Road
City Beijing
ZIP/Postal code 100094
Country China
 
Samples (8) GSM200010, GSM200011, GSM200012, GSM200013, GSM200014, GSM200015 
Series (1)
GSE8093 Differential Gene Expression Profile in Pig Adipose Tissue Treated with/without Clenbuterol

Data table header descriptions
ID
Block Block number of microarray
Column Column number of microarray
Row Row number of microarray
Name Name of microarray
Clone Number
X X axis of microarray
Y Y axis of microarray
SEQUENCE
GB_ACC GenBank Accession
SPOT_ID spot identifier

Data table
ID Block Column Row Name Clone Number X Y SEQUENCE GB_ACC SPOT_ID
1 1 1 1 1--4 rpfat_18755 1330 1360 CCCGGGCTGCAGGAATTCGGCACGAGCTTAACAAAAACATCCTTCATTTCTTTCAGACTTCAAGACTGCTTCCCTTCTTTATGTTTCTAGGATATTTATTTATGTATATTGATTATCATCCTTACACTTAGTAACCTTAATTTCCAGTGAAAATGGGGAAGTAGACAATTGTGAACTGTCACACATCATTCTGTAGTAGATTAACTTTGTAAATGTGCCACCTCAATTTATAGAAGTATATGCTTATAGTACGGG CLONE_NUMBER: rpfat_18755
2 1 2 1 1--4 rpfat_18755 1530 1370 CCCGGGCTGCAGGAATTCGGCACGAGCTTAACAAAAACATCCTTCATTTCTTTCAGACTTCAAGACTGCTTCCCTTCTTTATGTTTCTAGGATATTTATTTATGTATATTGATTATCATCCTTACACTTAGTAACCTTAATTTCCAGTGAAAATGGGGAAGTAGACAATTGTGAACTGTCACACATCATTCTGTAGTAGATTAACTTTGTAAATGTGCCACCTCAATTTATAGAAGTATATGCTTATAGTACGGG CLONE_NUMBER: rpfat_18755
3 1 3 1 1--4 rpfat_18755 1770 1370 CCCGGGCTGCAGGAATTCGGCACGAGCTTAACAAAAACATCCTTCATTTCTTTCAGACTTCAAGACTGCTTCCCTTCTTTATGTTTCTAGGATATTTATTTATGTATATTGATTATCATCCTTACACTTAGTAACCTTAATTTCCAGTGAAAATGGGGAAGTAGACAATTGTGAACTGTCACACATCATTCTGTAGTAGATTAACTTTGTAAATGTGCCACCTCAATTTATAGAAGTATATGCTTATAGTACGGG CLONE_NUMBER: rpfat_18755
4 1 4 1 1--8 rpfat_18787 1970 1370 GGCACGAGCTCGAACCCTCGTCTGAGCCTCTGTCTCCCCCTGTACACAGTGACGGGCTGGCTCAGAGCTTGGCCTTTCTGAGTCTTCCTTTTCAAGGCCACCTGGGCATCCAGTTGGCAGAAAGAGCAGAGAAGAGAAGGACTTAGAGCCGTGGACTCCCAGTGTCCACCCACCCACACTGGGCTCTGAGCCCCAGGCCTGACTCACTGACCCAAGAGCTGTCCCCTGATACTCCCGGGTCCCCCCCCCACTCCA CLONE_NUMBER: rpfat_18787
5 1 5 1 1--8 rpfat_18787 2210 1370 GGCACGAGCTCGAACCCTCGTCTGAGCCTCTGTCTCCCCCTGTACACAGTGACGGGCTGGCTCAGAGCTTGGCCTTTCTGAGTCTTCCTTTTCAAGGCCACCTGGGCATCCAGTTGGCAGAAAGAGCAGAGAAGAGAAGGACTTAGAGCCGTGGACTCCCAGTGTCCACCCACCCACACTGGGCTCTGAGCCCCAGGCCTGACTCACTGACCCAAGAGCTGTCCCCTGATACTCCCGGGTCCCCCCCCCACTCCA CLONE_NUMBER: rpfat_18787
6 1 6 1 1--8 rpfat_18787 2410 1380 GGCACGAGCTCGAACCCTCGTCTGAGCCTCTGTCTCCCCCTGTACACAGTGACGGGCTGGCTCAGAGCTTGGCCTTTCTGAGTCTTCCTTTTCAAGGCCACCTGGGCATCCAGTTGGCAGAAAGAGCAGAGAAGAGAAGGACTTAGAGCCGTGGACTCCCAGTGTCCACCCACCCACACTGGGCTCTGAGCCCCAGGCCTGACTCACTGACCCAAGAGCTGTCCCCTGATACTCCCGGGTCCCCCCCCCACTCCA CLONE_NUMBER: rpfat_18787
7 1 7 1 1--12 rpfat_17369 2650 1370 GCACGAGAAAAAACCTCTGGGTCTGGTCGTGGCTTCTCCACAGACGCGTTGGTGGTCACCTGGCAAGTTATTGACTCTTTCTGAACCACAGTTGTCAGGACTGTCAAATAGGATGGTGCTATCCGCCCTGATCATCTCACAGAGTCGGGGTGACCCTCAGATACCGGTGAACAGTGATCACATCTAGCTGTCAAGGTGCTAATTGATCCTTATTCTTTCAGAC CLONE_NUMBER: rpfat_17369
8 1 8 1 1--12 rpfat_17369 2870 1370 GCACGAGAAAAAACCTCTGGGTCTGGTCGTGGCTTCTCCACAGACGCGTTGGTGGTCACCTGGCAAGTTATTGACTCTTTCTGAACCACAGTTGTCAGGACTGTCAAATAGGATGGTGCTATCCGCCCTGATCATCTCACAGAGTCGGGGTGACCCTCAGATACCGGTGAACAGTGATCACATCTAGCTGTCAAGGTGCTAATTGATCCTTATTCTTTCAGAC CLONE_NUMBER: rpfat_17369
9 1 9 1 1--12 rpfat_17369 3090 1370 GCACGAGAAAAAACCTCTGGGTCTGGTCGTGGCTTCTCCACAGACGCGTTGGTGGTCACCTGGCAAGTTATTGACTCTTTCTGAACCACAGTTGTCAGGACTGTCAAATAGGATGGTGCTATCCGCCCTGATCATCTCACAGAGTCGGGGTGACCCTCAGATACCGGTGAACAGTGATCACATCTAGCTGTCAAGGTGCTAATTGATCCTTATTCTTTCAGAC CLONE_NUMBER: rpfat_17369
10 1 10 1 1--16 pfat_8175 3280 1370 TTTTTTTTTAGTTTGAAAAAGCAGAGTCAATATTTTATGTTGGAAAATGAAAACAATGTCAAATGTTGGTGAAATACAGACTGGAACATGCGTAGCTTTCAGATGCGGGATACAGGGGCGTGAGAAACCTTTCATTATCTGGGGTCAGTGGCCTCACAGAAGGAAAGCCACCTCCTTGTCCCCTTGAGGTACATGTGAGGTCCTCAGAGGAGGCCACCAAAGGACAACTTTAAAGTGCAGGTGCCCCTGGGAAAA CLONE_NUMBER: pfat_8175
11 1 11 1 1--16 pfat_8175 3510 1370 TTTTTTTTTAGTTTGAAAAAGCAGAGTCAATATTTTATGTTGGAAAATGAAAACAATGTCAAATGTTGGTGAAATACAGACTGGAACATGCGTAGCTTTCAGATGCGGGATACAGGGGCGTGAGAAACCTTTCATTATCTGGGGTCAGTGGCCTCACAGAAGGAAAGCCACCTCCTTGTCCCCTTGAGGTACATGTGAGGTCCTCAGAGGAGGCCACCAAAGGACAACTTTAAAGTGCAGGTGCCCCTGGGAAAA CLONE_NUMBER: pfat_8175
12 1 12 1 1--16 pfat_8175 3720 1370 TTTTTTTTTAGTTTGAAAAAGCAGAGTCAATATTTTATGTTGGAAAATGAAAACAATGTCAAATGTTGGTGAAATACAGACTGGAACATGCGTAGCTTTCAGATGCGGGATACAGGGGCGTGAGAAACCTTTCATTATCTGGGGTCAGTGGCCTCACAGAAGGAAAGCCACCTCCTTGTCCCCTTGAGGTACATGTGAGGTCCTCAGAGGAGGCCACCAAAGGACAACTTTAAAGTGCAGGTGCCCCTGGGAAAA CLONE_NUMBER: pfat_8175
13 1 13 1 1--20 rpigfat_10350 3940 1370 TGATGTAAATACTTAGAAACACTAATAATATAACTAATAATCAGTTTGACTCTGTGTATATGAATGCGTGTGTTTGATGAAAAGTTTGTATAGCCCTCTGAAAGTTTTAAAAATGCGTTAAATCACTTCATCTTTTCATATTTTATTGTCTGCTCAAGGTTCTTTTACACAGTGTCATCCTTTATAGGGTATAATAGGGATGATCAACCTACAGCAGCAGTAAGGAGATGATACTCAATGCCGGAGAAGTCG CLONE_NUMBER: rpigfat_10350
14 1 14 1 1--20 rpigfat_10350 4190 1370 TGATGTAAATACTTAGAAACACTAATAATATAACTAATAATCAGTTTGACTCTGTGTATATGAATGCGTGTGTTTGATGAAAAGTTTGTATAGCCCTCTGAAAGTTTTAAAAATGCGTTAAATCACTTCATCTTTTCATATTTTATTGTCTGCTCAAGGTTCTTTTACACAGTGTCATCCTTTATAGGGTATAATAGGGATGATCAACCTACAGCAGCAGTAAGGAGATGATACTCAATGCCGGAGAAGTCG CLONE_NUMBER: rpigfat_10350
15 1 15 1 1--20 rpigfat_10350 4410 1370 TGATGTAAATACTTAGAAACACTAATAATATAACTAATAATCAGTTTGACTCTGTGTATATGAATGCGTGTGTTTGATGAAAAGTTTGTATAGCCCTCTGAAAGTTTTAAAAATGCGTTAAATCACTTCATCTTTTCATATTTTATTGTCTGCTCAAGGTTCTTTTACACAGTGTCATCCTTTATAGGGTATAATAGGGATGATCAACCTACAGCAGCAGTAAGGAGATGATACTCAATGCCGGAGAAGTCG CLONE_NUMBER: rpigfat_10350
16 1 16 1 1--24 rpfat_11004 4620 1370 AAAAAAAAAAAAAAAAAAAAGATAAAGAACTGTAACTAAGGGAGGTCACATTGGGATTGGATCCCAGCCCAAAAGGGAGAGAAAAAGAGCCTAAGAATACTGTACTAGCAGATACTATTTGAGGCAGAAAAGAGAAAGAGTCCTCAAGGGCTGAGAGACAGAAAAGACCAACTAAGAATAAAAAAAAAAAAAAAAAAAATT CLONE_NUMBER: rpfat_11004
17 1 17 1 1--24 rpfat_11004 4840 1420 AAAAAAAAAAAAAAAAAAAAGATAAAGAACTGTAACTAAGGGAGGTCACATTGGGATTGGATCCCAGCCCAAAAGGGAGAGAAAAAGAGCCTAAGAATACTGTACTAGCAGATACTATTTGAGGCAGAAAAGAGAAAGAGTCCTCAAGGGCTGAGAGACAGAAAAGACCAACTAAGAATAAAAAAAAAAAAAAAAAAAATT CLONE_NUMBER: rpfat_11004
18 1 18 1 1--24 rpfat_11004 5060 1370 AAAAAAAAAAAAAAAAAAAAGATAAAGAACTGTAACTAAGGGAGGTCACATTGGGATTGGATCCCAGCCCAAAAGGGAGAGAAAAAGAGCCTAAGAATACTGTACTAGCAGATACTATTTGAGGCAGAAAAGAGAAAGAGTCCTCAAGGGCTGAGAGACAGAAAAGACCAACTAAGAATAAAAAAAAAAAAAAAAAAAATT CLONE_NUMBER: rpfat_11004
19 1 1 2 1--100 rpfat_16335 1320 1570 CCCCGGGCTGCAGGAATTCGGCACGAGATTCTCTTGGAAACTATGTGACGAGAAAGGACTGAATTTGCGTGCCGTTAGCCGCAGGGTTAAGCTCGCCTCTGGATGTGGGCCGTCCGTACTGCACCTTTTCAGAAGCGCAGCCGCCCTGGATCTACCCAGCCGCAGTGACTCCATGTAAGCTGCTGCTACACAGGTGGCTTTCGTTCTGCTCCCTCCACTAGTTGTGTAGAGCCTGGTGGCGGGTACGAAGGGCGC CLONE_NUMBER: rpfat_16335
20 1 2 2 1--100 rpfat_16335 1540 1570 CCCCGGGCTGCAGGAATTCGGCACGAGATTCTCTTGGAAACTATGTGACGAGAAAGGACTGAATTTGCGTGCCGTTAGCCGCAGGGTTAAGCTCGCCTCTGGATGTGGGCCGTCCGTACTGCACCTTTTCAGAAGCGCAGCCGCCCTGGATCTACCCAGCCGCAGTGACTCCATGTAAGCTGCTGCTACACAGGTGGCTTTCGTTCTGCTCCCTCCACTAGTTGTGTAGAGCCTGGTGGCGGGTACGAAGGGCGC CLONE_NUMBER: rpfat_16335

Total number of rows: 11520

Table truncated, full table size 1143 Kbytes.




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