The dataset contains unique, 20-25 nucleotide small RNA sequences identified using high-throughput 454 sequencing of samples prepared from Arabidopsis thaliana. Each sequence has a minimum of one genomic locus. Small RNAs were sequenced from inflorescence tissue (containing stage 1-12 flowers) of wildtype Col-0 plants, mutants with defects in each DCL gene (dcl1-7, dcl2-1, dcl3-1, dcl4-2), mutants with defects in each RDR gene for which a function has been established (rdr1-1, rdr2-1, rdr6-15), and whole seedling tissue (3 day post-germinations) of Col-0 and rdr6-15 plants. Small RNA preparations from leaf samples of Col-O that were either uninoculated or inoculated by Pseudomonas syringae pv tomato (DC3000hrcC) for 1 hr and 3 hr were also sequenced.