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Platform GPL4464 Query DataSets for GPL4464
Status Public on Nov 23, 2006
Title UMN Mouse 11K V 11_22_04 BMAP
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Mus musculus
Manufacturer Bio-Medical Genomics Center, University of Minnesota.
Manufacture protocol Basic protocol for generation of mouse microarrays
i) Obtain cDNA library
(1) thousands of bacterial stocks in 96-well plates
(2) each bacterial stock contains a standard eukaryotic expression
plasmid that contains a unique stretch of mouse cDNA inserted in
the multiple cloning site
ii) Replicate the library
iii) PCR amplify each cDNA clone in 96 well reactions
iv) Check PCR reactions on an agarose gel
v) Purify PCR reactions with a Millipore filter
vi) Elute purified DNA with 3XSSC (spotting buffer)
vii) Spot DNA onto glass slides using a DNA spotting robot
 
 
Contributor(s) Yekkirala A
Submission date Oct 18, 2006
Last update date Nov 23, 2006
Contact name Ajay Yekkirala
Organization name University of Minnesota
Street address 308 Harvard St 8-120
City Minneapolis
State/province MN
ZIP/Postal code 55112
Country USA
 
Samples (12) GSM141569, GSM142384, GSM142385, GSM142386, GSM142387, GSM142388 
Series (2)
GSE6479 Analgesia of naloxone and morphine in wild-type and NY1DD sickle cell mice using BMAP cDNA microarrays
GSE6578 Analgesia of naloxone in wild-type and NY1DD transgenic mouse model of sickle cell anemia using BMAP cDNA microarrays

Data table header descriptions
ID
release_plate DNA sample transfered from these 96-well plates
release_row Row of the 96-well plate A-H
release_col Columns of the well plate 1-12
96WellName Location of the spot: plate #, Row # and column #.
384SortTool Samples were sorted into 384-well plates.
384plate 384-well plate #
384row Row of the 384-well plate
384column Column of the 384-well plate.
cluster_id
cdna_id
acc Genbank accession No.
nid
pid
clone_end
Amplified for microarray? (Y) yes, (N) no, or (W) weakly
title Description of the gene
gene Gene symbol
chrom
build_version Unigene build version
species Mus Musculus
library_name Mus Musculus
vector_name PT7T3D vector
antibiotic amp
GB_ACC GenBank accession
SPOT_ID spot identifer

Data table
ID release_plate release_row release_col 96WellName 384SortTool 384plate 384row 384column cluster_id cdna_id acc nid pid clone_end Amplified for microarray? title gene chrom build_version species library_name vector_name antibiotic GB_ACC SPOT_ID
1a1 1 a 1 1a1 101.1 1 a 1 x UI-M-AQ0-aaa-a-01-6-UI AI835180 x x 3 Y Not available at this time x x x mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835180
1b1 1 b 1 1b1 101.2 1 a 2 Mm.17815 UI-M-AQ0-aaa-d-11-6-UI AI835214 g5469427 x 3 Y ESTs x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835214
1a2 1 a 2 1a2 102.1 1 a 3 Mm.28312 UI-M-AQ0-aaa-b-03-6-UI AI835190 g5469403 x 3 Y ESTs x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835190
1b2 1 b 2 1b2 102.2 1 a 4 Mm.4201 UI-M-AQ0-aaa-e-02-6-UI AI835216 g5469429 x 3 Y Keratin complex 1, acidic, gene 12 Krt1-12 11 unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835216
1a3 1 a 3 1a3 103.1 1 a 5 Mm.4926 UI-M-AQ0-aaa-b-04-6-UI AI835191 g5469404 x 3 Y M.musculus pacsin gene x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835191
1b3 1 b 3 1b3 103.2 1 a 6 Mm.28203 UI-M-AQ0-aaa-e-09-6-UI AI835221 g5469434 x 3 Y ESTs, Moderately similar to HIGH MOBILITY GROUP-LIKE NUCLEAR PROTEIN 2 [Saccharomyces cerevisiae] x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835221
1a4 1 a 4 1a4 104.1 1 a 7 Mm.28782 UI-M-AQ0-aaa-b-07-6-UI AI835193 g5469406 x 3 Y Mus musculus CIPER protein (Ciper) mRNA, complete cds x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835193
1b4 1 b 4 1b4 104.2 1 a 8 Mm.26304 UI-M-AQ0-aaa-f-07-6-UI AI835245 g5469458 x 3 Y ESTs x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835245
1a5 1 a 5 1a5 105.1 1 a 9 Mm.32310 UI-M-AQ0-aaa-b-08-6-UI AI835194 g5469407 x 3 Y ESTs x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835194
1b5 1 b 5 1b5 105.2 1 a 10 Mm.29899 UI-M-AQ0-aaa-f-08-6-UI AI835229 g5469442 x 3 Y ESTs, Moderately similar to HH0712 cDNA clone for KIAA0442 has a 574-bp insertion at position 1474 of the sequence of KIAA0442. [H.sapiens] x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835229
1a6 1 a 6 1a6 106.1 1 a 11 Mm.60576 UI-M-AQ0-aaa-b-12-6-UI AI835197 g5469410 x 3 Y ESTs x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835197
1b6 1 b 6 1b6 106.2 1 a 12 Mm.39108 UI-M-AQ0-aaa-f-09-6-UI AI835246 g5469459 x 3 Y EST x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835246
1a7 1 a 7 1a7 107.1 1 a 13 Mm.8739 UI-M-AQ0-aaa-c-06-6-UI AI835201 g5469414 x 3 Y Sarcoglycan, epsilon Sgce 6 unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835201
1b7 1 b 7 1b7 107.2 1 a 14 Mm.34747 UI-M-AQ0-aaa-f-11-6-UI AI835231 g5469444 x 3 N Mus musculus pancreas sodium bicarbonate cotransporter mRNA, complete cds x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835231
1a8 1 a 8 1a8 108.1 1 a 15 Mm.24104 UI-M-AQ0-aaa-c-10-6-UI AI835204 g5469417 x 3 Y ESTs, Moderately similar to HSPC011 [H.sapiens] x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835204
1b8 1 b 8 1b8 108.2 1 a 16 Mm.21002 UI-M-AQ0-aaa-h-02-6-UI AI835238 g5469451 x 3 N Solute carrier family 2 (facilitated glucose transporter), member 1 Slc2a1 4 unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835238
1a9 1 a 9 1a9 109.1 1 a 17 Mm.15675 UI-M-AQ0-aaa-c-12-6-UI AI835206 g5469419 x 3 Y Ephrin A1 Efna1 3 unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835206
1b9 1 b 9 1b9 109.2 1 a 18 Mm.28462 UI-M-AQ0-aaa-h-10-6-UI AI835242 g5469455 x 3 Y ESTs, Weakly similar to aminopeptidase-B [R.norvegicus] x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835242
1a10 1 a 10 1a10 110.1 1 a 19 Mm.39755 UI-M-AQ0-aaa-d-02-6-UI AI835207 g5469420 x 3 N EST x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835207
1b10 1 b 10 1b10 110.2 1 a 20 Mm.41560 UI-M-AQ0-aab-a-02-6-UI AI835248 g5469461 x 3 Y Mus musculus mRNA for neuroserpin x x unigene_build69 mouse non-normalized library from mouse (27-32 day; C57BL/6J; mixture of male and female) hippocampus PT7T3D amp AI835248

Total number of rows: 11532

Table truncated, full table size 2349 Kbytes.






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