Microarrays are printed on UltraGAPS™ slides (Corning Inc.) using high-precision robotics. Printed arrays are processed following Corning's protocol and are ready-to-use. Single-spotted array containing 19,008 characterized and unknown human ESTs. The clone set has been sequence-verified at the Microarray Centre After printing, the slides are subjected to processing and performance testing based on true labelling, hybridization and scanning procedures. To ensure quality performance of microarrays, printed slides that are less than 95% perfect (in printing fidelity and spot integrity) are not released to users.
Profiling of genes regulated by TGF-beta in immortalized skin keratinocyte cells(HaCaT).
Data table header descriptions
ID
Block
Array Block number
Row
Spot Row Number (on the horizontal axis) in a specified Block
Column
Spot Column Number (on the vertical axis) in a specified Block
UHN_ID
This is the IMAGE clone ID (when available) that is returned based upon the sequence data (this may or may not match the Original_Image_ID). We have been unsuccessful in sequencing a small percentage of clones. These have been annotated “No_Seq”. There are also cases where the sequence did not return a confident hit. These are annotated “Unresolved”. Finally, there are cases where a confident hit is returned that do not have an IMAGE clone ID. These are annotated “N/A” and usually have an Accession number and UniGene ID.
Accession ID
The AccessionID of the matching sequence. E-values, match length, %ID values in later columns are based upon blast results of our sequence with the sequence of this Accession.
UNIGENE Cluster
the UnigeneID returned from Stanford Source (http://genome-www5.stanford.edu/cgi-bin/SMD/source/sourceSearch) based upon the verified ImageID or AccessionID (search performed on February 9, 2004).
ORF
Symbol for the UniGene cluster
Chromosome
Chr. Location for the UniGene cluster
Cytoband
Cytoband for the UniGene cluster
CLONE_ID
This is the IMAGE clone ID that came with the plate originally acquired. You can search with this to correlate previous versions of H19k data with the new annotations
Less...
More...