Custom Affymetrix GeneChip designed by Eos Biotechnology Inc. to measure the expression of a large number of genes or ESTs on a single array. This is achieved by choosing those probes from within each set of 20 Affymetrix perfect match probes that show the least random fluctuation relative to the perceived specific hybridization over a wide ranging set of samples (Glynne et al. 2000). Experiments undertaken by Eos have revealed that the mismatch probes do not increase sensitivity or reproducibility (RJG and GG unpublished data). Consequently, each gene on the Eos array is represented by 6-8 perfect match probes, as opposed to 20 perfect match and 20 mismatch probes, increasing the number of genes for which expression can be assayed in parallel to >39,000.
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