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Platform GPL1262 Query DataSets for GPL1262
Status Public on May 27, 2004
Title NCI/ATC Hs-UniGEM2
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Homo sapiens
Manufacturer Advanced Technology Center Microarray Facility (NIH/NCI/CCR/ATC)
Manufacture protocol Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com). The human cDNA probe set is based on 9128 PCR products from Incyte's human UniGEM 2.0 library (http://www.incyte.com). The set includes 122 Incyte EST clones; 8998 identifed with a public GenBank Accession; 8905 Unique GenBank Accessions; 8882 mapped into Hs UniGene Build #131; 8281 Unique UniGene Clusters; 1179 EST clusters; 7102 Named clusters. The cDNA probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml. The microarray spot densities are 235 microns (center-to-center), and one spot is printed per probe per slide. The microarrays printed on Corning CMT-GAPS II slides (Item #40004) coated with amino-silane and post-processed according to the manufacturer's protocol.
Support glass
Coating aminosilane
 
Description Printing by the NCI /ATC Microarray Facility
Surface type: amino-silane coated glass Corning CMT-GAPS II (http://www.corning.com)
Attachment: UV cross-linked
Array description: The custom arrays are manufactured by the NCI Advanced Technology Center Microarray Facility (NIH/NCI/CCR/ATC). Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com). The human cDNA probe set is based on 9128 PCR products from Incyte's human UniGEM 2.0 library (http://www.incyte.com). The set includes 122 Incyte EST clones; 8998 identifed with a public GenBank Accession; 8905 Unique GenBank Accessions; 8882 mapped into Hs UniGene Build #131; 8281 Unique UniGene Clusters; 1179 EST clusters; 7102 "Named" clusters.The cDNA probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml. The microarray spot densities are 235 microns (center-to-center), and one spot is printed per probe per slide. The microarrays printed on Corning CMT-GAPS II slides (Item #40004) coated with amino-silane and post-processed according to the manufacturer's protocol.
 
Submission date May 25, 2004
Last update date Jan 17, 2013
Contact name David Petersen
E-mail(s) petersed@mail.nih.gov
Phone 301-435-7898
Organization name NCI
Lab NCI Microarray Facility
Street address 8717 Grovemont Circle
City Gaithersburg
State/province MD
ZIP/Postal code 20892-4605
Country USA
 
Samples (152) GSM24324, GSM24325, GSM24326, GSM24327, GSM24328, GSM24331 
Series (5)
GSE1441 Host Cell Gene Expression During HIV-1 Latency and Reactivation
GSE1443 Host Cell Gene Expression During HIV-1 Latency in Chronically Infected Cell Lines
GSE4982 Dye Bias Array Set

Data table header descriptions
ID NIH mAdb well id plus replicate number
MADB_WELL_ID NIH mAdb plate-well identifier for clone sample
CLONE_ID Clone identifier
GENE Gene Name
UNIGENE UniGene Identifier Build #Hs.201
DESCRIPTION Gene Description
GB_ACC GENBANK ID assigned by vendor
SPOT_ID alternative spot identifier

Data table
ID MADB_WELL_ID CLONE_ID GENE UNIGENE DESCRIPTION GB_ACC SPOT_ID
159769_1 159769 IncytePD:1993847 TNFRSF1B 256278 Tumor necrosis factor receptor superfamily, member 1B AL031276
159777_1 159777 IncytePD:1593787 gamma-aminobutyric acid (GABA) A receptor, beta 1 M59216
159785_1 159785 IncytePD:1861378 ATP5G1 80986 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9) NM_005175
159865_1 159865 IncytePD:2135063 CYB5B 461131 Cytochrome b5 type B (outer mitochondrial membrane) BE409876
159873_1 159873 IncytePD:1633249 CBR1 88778 Carbonyl reductase 1 AB003151
159881_1 159881 IncytePD:1880710 CCDC130 24998 Coiled-coil domain containing 130 X13956
159961_1 159961 IncytePD:2174563 DAP3 516746 Death associated protein 3 AA207194
159969_1 159969 IncytePD:1664320 C1R 524224 Complement component 1, r subcomponent M14058
159977_1 159977 IncytePD:1900231 ACTN1 509765 Actinin, alpha 1 X15804
160057_1 160057 IncytePD:2208152 ATF2 654398 Activating transcription factor 2 NM_001880
160065_1 160065 IncytePD:1678071 NR3C2 163924 Nuclear receptor subfamily 3, group C, member 2 NM_000901
160073_1 160073 IncytePD:1976179 PAEP 532325 Progestagen-associated endometrial protein (placental protein 14, pregnancy-associated endometrial alpha-2-globulin, alpha uterine protein) M61886
160153_1 160153 IncytePD:689078 HESX1 171980 HESX homeobox 1 AA151810
160161_1 160161 IncytePD:2059256 mitogen-activated protein kinase 14 Z95152
160169_1 160169 IncytePD:1464211 CTRL 654546 Chymotrypsin-like X71877
160249_1 160249 IncytePD:702628 CDC6 405958 Cell division cycle 6 homolog (S. cerevisiae) AF022109
160257_1 160257 IncytePD:2059955 SCAP 531789 SREBF chaperone D83782
160265_1 160265 IncytePD:1739722 PFKP 26010 Phosphofructokinase, platelet BE378739
160345_1 160345 IncytePD:754239 PCTK3 445402 PCTAIRE protein kinase 3 BE258493
160353_1 160353 IncytePD:2060327 ATP5I 85539 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E NM_007100

Total number of rows: 9984

Table truncated, full table size 854 Kbytes.






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