RACK1 receptor for activated C kinase 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: RACK1provided by HGNC
Official Full Name: receptor for activated C kinase 1provided by HGNC
Primary source: HGNC:HGNC:4399
See related: Ensembl:ENSG00000204628 MIM:176981; AllianceGenome:HGNC:4399
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
Summary: Enables several functions, including cyclin binding activity; enzyme binding activity; and protein domain specific binding activity. Involved in several processes, including regulation of protein metabolic process; regulation of signal transduction; and regulation of vesicle-mediated transport. Located in several cellular components, including midbody; perinuclear region of cytoplasm; and phagocytic cup. Part of IRE1-RACK1-PP2A complex. [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in ovary (RPKM 1262.3), lymph node (RPKM 598.8) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 5q35.3

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	5	NC_000005.10 (181236897..181243906, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	5	NC_060929.1 (181796848..181803858, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	5	NC_000005.9 (180663897..180670906, complement)

Chromosome 5 - NC_000005.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Identification of RACK1 as a novel regulator of non-structural protein 4 of chikungunya virus.
Title: Identification of RACK1 as a novel regulator of non-structural protein 4 of chikungunya virus.
PIN1 promotes the metastasis of cholangiocarcinoma cells by RACK1-mediated phosphorylation of ANXA2.
Title: PIN1 promotes the metastasis of cholangiocarcinoma cells by RACK1-mediated phosphorylation of ANXA2.
Deciphering the neuroprotective mechanisms of RACK1 in cerebral ischemia-reperfusion injury: Pioneering insights into mitochondrial autophagy and the PINK1/Parkin axis.
Title: Deciphering the neuroprotective mechanisms of RACK1 in cerebral ischemia-reperfusion injury: Pioneering insights into mitochondrial autophagy and the PINK1/Parkin axis.
OTUB1 accelerates hepatocellular carcinoma by stabilizing RACK1 via its non-canonical ubiquitination.
Title: OTUB1 accelerates hepatocellular carcinoma by stabilizing RACK1 via its non-canonical ubiquitination.
RACK1 promotes the occurrence and progression of cervical carcinoma.
Title: RACK1 promotes the occurrence and progression of cervical carcinoma.
Loss of RACK1 promotes glutamine addiction via activating AKT/mTOR/ASCT2 axis to facilitate tumor growth in gastric cancer.
Title: Loss of RACK1 promotes glutamine addiction via activating AKT/mTOR/ASCT2 axis to facilitate tumor growth in gastric cancer.
Neuropilin 1 promotes unilateral ureteral obstruction-induced renal fibrosis via RACK1 in renal tubular epithelial cells.
Title: Neuropilin 1 promotes unilateral ureteral obstruction-induced renal fibrosis via RACK1 in renal tubular epithelial cells.
Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development.
Title: Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development.
Targeting RACK1 to alleviate TDP-43 and FUS proteinopathy-mediated suppression of protein translation and neurodegeneration.
Title: Targeting RACK1 to alleviate TDP-43 and FUS proteinopathy-mediated suppression of protein translation and neurodegeneration.
Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression.
Title: Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression.

Submit: New GeneRIF Correction See all GeneRIFs (222)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	Tandem affinity purification and mass spectrometry analysis identify subunit beta polypeptide 2-like 1 of G protein (GNB2L1, RACK1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Gag-Pol	gag-pol	Tandem affinity purification and mass spectrometry analysis identify subunit beta polypeptide 2-like 1 of G protein (GNB2L1, RACK1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Nef	nef	Tandem affinity purification and mass spectrometry analysis identify subunit beta polypeptide 2-like 1 of G protein (GNB2L1, RACK1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
	nef	The binding of HIV-1 Nef with Rack1 (receptor for activated C kinase 1) is mediated by amino acids 34-143 in Nef; Rack1 acts as a Nef intracellular docking site, bringing Nef and PKCs together	PubMed
Pr55(Gag)	gag	Tandem affinity purification and mass spectrometry analysis identify subunit beta polypeptide 2-like 1 of G protein (GNB2L1, RACK1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Rev	rev	HIV-1 Rev interacting protein, guanine nucleotide binding protein, beta polypeptide 2-like 1 (GNB2L1), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells	PubMed
Tat	tat	Guanine nucleotide binding protein subunit beta polypeptide 2-like 1 (GNB2L1) is identified to interact with HIV-1 Tat mutant Nullbasic in HeLa cells by LC MS/MS	PubMed
matrix	gag	Exposure of human acute monocytic leukemia cells THP-1 to HIV-1 MA activates the syndecan-2/RACK-1/Jak-1 signal pathway, leading to STAT-1 phosphorylation	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D5S2354 (e-PCR)
- UniSTS:473392

D8S2278 (e-PCR)
- UniSTS:473906

D8S2279 (e-PCR)
- UniSTS:473907

SGC34615 (e-PCR)
- UniSTS:36423

G32754 (e-PCR)
- UniSTS:117335

L18426 (e-PCR)
- UniSTS:34648

D1S1425 (e-PCR)
- UniSTS:149621

A009P44 (e-PCR)
- UniSTS:14494

RH17921 (e-PCR)
- UniSTS:22450

10STS02 (e-PCR)
- UniSTS:504500

WI-12627 (e-PCR)
- UniSTS:65215

D5S1561E (e-PCR)
- UniSTS:150993

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables BH3 domain binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	HDA more info	PubMed
enables SH2 domain binding	IDA Inferred from Direct Assay more info	PubMed
enables cadherin binding	HDA more info	PubMed
enables cyclin binding	IPI Inferred from Physical Interaction more info	PubMed
enables cysteine-type endopeptidase activator activity involved in apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ion channel inhibitor activity	ISS Inferred from Sequence or Structural Similarity more info
enables molecular adaptor activity	TAS Traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase C binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase C binding	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphatase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine kinase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables receptor tyrosine kinase binding	IDA Inferred from Direct Assay more info	PubMed
enables ribosome binding	IBA Inferred from Biological aspect of Ancestor more info
enables ribosome binding	IDA Inferred from Direct Assay more info	PubMed
enables signaling adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables signaling receptor binding	NAS Non-traceable Author Statement more info	PubMed
enables translation regulator activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in activation of cysteine-type endopeptidase activity involved in apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cellular response to glucose stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to growth factor stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in cytoplasmic translation	NAS Non-traceable Author Statement more info	PubMed
involved_in gastrulation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of Wnt signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of cell growth	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of endoplasmic reticulum unfolded protein response	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of peptidyl-serine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phagocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein binding	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of translation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of translational frameshifting	IBA Inferred from Biological aspect of Ancestor more info
involved_in pigmentation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of GTPase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of Golgi to plasma membrane protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell migration	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of gastrulation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of intrinsic apoptotic signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitochondrial depolarization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein-containing complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell division	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of establishment of cell polarity	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of protein localization	ISS Inferred from Sequence or Structural Similarity more info
involved_in rescue of stalled ribosome	IBA Inferred from Biological aspect of Ancestor more info
involved_in rescue of stalled ribosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rhythmic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of IRE1-RACK1-PP2A complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	NAS Non-traceable Author Statement more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info
located_in cytosol	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
part_of cytosolic small ribosomal subunit	NAS Non-traceable Author Statement more info	PubMed
located_in dendrite	ISS Inferred from Sequence or Structural Similarity more info
located_in extracellular exosome	HDA more info	PubMed
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed
located_in neuronal cell body	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perikaryon	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in phagocytic cup	IDA Inferred from Direct Assay more info	PubMed
part_of small ribosomal subunit	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: small ribosomal subunit protein RACK1

Names: cell proliferation-inducing gene 21 protein; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; guanine nucleotide binding protein beta polypeptide 2-like 1; guanine nucleotide-binding protein subunit beta-2-like 1; guanine nucleotide-binding protein subunit beta-like protein 12.3; human lung cancer oncogene 7 protein; lung cancer oncogene 7; proliferation-inducing gene 21; protein homologous to chicken B complex protein, guanine nucleotide binding; receptor of activated protein C kinase 1; receptor of activated protein kinase C 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_006098.5 → NP_006089.1 small ribosomal subunit protein RACK1

See identical proteins and their annotated locations for NP_006089.1

Status: VALIDATED

Source sequence(s)

AC008443, AK222488, BC029996, M24194

Consensus CDS

CCDS34324.1

UniProtKB/Swiss-Prot

B3KTJ0, D3DWS0, P25388, P63244, P99049, Q53HU2, Q5J8M6, Q5VLR4, Q6FH47

UniProtKB/TrEMBL

D6RAC2, E9KL35

Related

ENSP00000426909.1, ENST00000512805.6

Conserved Domains (2) summary

cd00200
Location:7 → 311

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:18 → 61

7WD40; WD40 repeat [structural motif]