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    spag spaghetti [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 43958, updated on 18-Sep-2024

    Summary

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    Official Symbol
    spagprovided by FlyBase
    Official Full Name
    spaghettiprovided by FlyBase
    Primary source
    FLYBASE:FBgn0015544
    Locus tag
    Dmel_CG13570
    See related
    AllianceGenome:FB:FBgn0015544
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG13570; Dmel\CG13570; l(2)k12101; Rpap3; Spag; SPAG; spagh
    Summary
    Predicted to enable Hsp70 protein binding activity and Hsp90 protein binding activity. Involved in negative regulation of motor neuron apoptotic process and regulation of locomotor rhythm. Predicted to be located in cytoplasm. Is expressed in adult head. Orthologous to human RPAP3 (RNA polymerase II associated protein 3). [provided by Alliance of Genome Resources, Apr 2022]
    Orthologs
    all
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    Genomic context

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    See spag in Genome Data Viewer
    Location:
    60C1-60C1; 2-106 cM
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (24159384..24161503)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (20046907..20049026)

    Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene long non-coding RNA:CR44826 Neighboring gene speck Neighboring gene DnaJ-like-60 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene mitochondrial ribosomal protein S17

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Drosophila DBT Autophosphorylation of Its C-Terminal Domain Antagonized by SPAG and Involved in UV-Induced Apoptosis. Fan JY, et al. Mol Cell Biol, 2015 Jul. PMID 25939385, Free PMC Article
    2. The DnaJ60 gene of Drosophila melanogaster encodes a new member of the DnaJ family of proteins. Iliopoulos I, et al. Biol Chem, 1997 Oct. PMID 9372188
    3. Spaghetti, a homolog of human RPAP3 (RNA polymerase II-associated protein 3), determines the fate of germline stem cells in Drosophila ovary. Chen D, et al. Cell Biol Int, 2018 Jul. PMID 29110400
    4. Drosophila spaghetti and doubletime link the circadian clock and light to caspases, apoptosis and tauopathy. Means JC, et al. PLoS Genet, 2015 May. PMID 25951229, Free PMC Article
    5. A Drosophila immune response against Ras-induced overgrowth. Hauling T, et al. Biol Open, 2014 Apr 15. PMID 24659248, Free PMC Article

    See all (54) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Heterozygous mutations in hsp90 (hsp90/+) dramatically accelerated the GSC loss in spag RNAi ovaries
      Title: Spaghetti, a homolog of human RPAP3 (RNA polymerase II-associated protein 3), determines the fate of germline stem cells in Drosophila ovary.
    2. we show that spag knock-down or reduced Dbt activity in circadian cells leads to longer circadian periods and expression of activated initiator caspase only during the middle of the day.
      Title: Drosophila spaghetti and doubletime link the circadian clock and light to caspases, apoptosis and tauopathy.
    3. Data show that the HSP-90 cochaperone spaghetti protein (SPAG) antagonizes casein kinase I epsilon DBT autophosphorylation in S2 cells
      Title: Drosophila DBT Autophosphorylation of Its C-Terminal Domain Antagonized by SPAG and Involved in UV-Induced Apoptosis.
    4. Interaction of Spag with both Hsp70 and Hsp90 suggests a model whereby R2TP would accompany clients from Hsp70 to Hsp90 to facilitate their assembly into macromolecular complexes.
      Title: Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables Hsp70 protein binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables Hsp90 protein binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    involved_in negative regulation of motor neuron apoptotic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of locomotor rhythm IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  

    General protein information

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    Preferred Names
    spaghetti
    Names
    CG13570-PA
    spag-PA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033778.4 Reference assembly

      Range
      24159384..24161503
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_079925.3 → NP_524664.1  spaghetti [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_524664.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q86PC1, Q9V3E9
      Related
      FBpp0072164
      Conserved Domains (6) summary
      smart00028
      Location:130 → 160
      TPR; Tetratricopeptide repeats
      sd00006
      Location:96 → 124
      TPR; TPR repeat [structural motif]
      pfam00515
      Location:102 → 129
      TPR_1; Tetratricopeptide repeat
      pfam13414
      Location:95 → 161
      TPR_11; TPR repeat
      pfam13432
      Location:136 → 197
      TPR_16; Tetratricopeptide repeat
      pfam13877
      Location:415 → 503
      RPAP3_C; Potential Monad-binding region of RPAP3
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q86PC1 Q86PC1
    Q9V3E9 Q9V3E9

    Gene LinkOut

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