crb crumbs [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: crbprovided by FlyBase
Official Full Name: crumbsprovided by FlyBase
Primary source: FLYBASE:FBgn0259685
Locus tag: Dmel_CG6383
See related: AllianceGenome:FB:FBgn0259685
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 0509/20; 1384/04; CG6383; Crb; CRB; Crbs; crumb; Crumbs; CT19912; DmCrb; Dmel\CG6383; far; l(3)07207; l(3)j1B5; l(3)S050920; l(3)S058104
Summary: Enables protein kinase C binding activity. Involved in several processes, including adherens junction organization; morphogenesis of an epithelium; and regulation of organ growth. Located in several cellular components, including apicolateral plasma membrane; photoreceptor connecting cilium; and rhabdomere. Is expressed in several structures, including epithelium; gut section; male reproductive system; presumptive embryonic/larval system; and rhabdomeric photoreceptor cell. Used to study Leber congenital amaurosis and retinitis pigmentosa. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); focal segmental glomerulosclerosis 9; pigmented paravenous chorioretinal atrophy; retinal disease (multiple); and ventriculomegaly - cystic kidney disease. Orthologous to several human genes including CRB1 (crumbs cell polarity complex component 1). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 95F10-95F11; 3-84 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (24295078..24314541)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (20120800..20140263)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Crumbs complex-directed apical membrane dynamics in epithelial cell ingression.
Title: Crumbs complex-directed apical membrane dynamics in epithelial cell ingression.
Crumbs and the apical spectrin cytoskeleton regulate R8 cell fate in the Drosophila eye.
Title: Crumbs and the apical spectrin cytoskeleton regulate R8 cell fate in the Drosophila eye.
Ciao1 interacts with Crumbs and Xpd to regulate organ growth in Drosophila.
Title: Ciao1 interacts with Crumbs and Xpd to regulate organ growth in Drosophila.
Drosophila Neuroblast Selection Is Gated by Notch, Snail, SoxB, and EMT Gene Interplay.
Title: Drosophila Neuroblast Selection Is Gated by Notch, Snail, SoxB, and EMT Gene Interplay.
Crumbs as an essential hub required to maintain the organization of the apical membrane and the physiological activity of the larval salivary gland.
Title: Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P(2) in Drosophila.
Results show that Crb redistributes to the subapical region during pupal wing development and is not required for apical/basal and planar polarities of the pupal wing epithelium, but participates in prehair formation and hexagonal packing during morphogenesis. Crb is required during wing morphogenesis for the integrity and stability of adherens junctions and circumferential actomyosin belt.
Title: Crumbs, Moesin and Yurt regulate junctional stability and dynamics for a proper morphogenesis of the Drosophila pupal wing epithelium.
Consistent with the role of Crb in apical membrane growth, RNAi-mediated knockdown of Crb results in decreased apical surface growth of tracheal cells and impaired axial elongation of the dorsal trunk. We conclude that Apn is a novel regulator of tracheal tube expansion in larval tracheae, the function of which is mediated by Crb.
Title: The apical protein Apnoia interacts with Crumbs to regulate tracheal growth and inflation.
Suggest a model in which Src42A would sense the inherent anisotropic mechanical tension of the tracheal tube and translate it into a polarised Crumbs accumulation, which may promote a bias towards longitudinal membrane expansion, orienting cell elongation and, as a consequence, longitudinal growth at the tissue level.
Title: Anisotropic Crb accumulation, modulated by Src42A, is coupled to polarised epithelial tube growth in Drosophila.
Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues, is reported.
Title: A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
Results demonstrate that the apical membrane localization of Crumbs is promoted by Dlg5.
Title: Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis.

Submit: New GeneRIF Correction See all GeneRIFs (62)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables Notch binding	IBA Inferred from Biological aspect of Ancestor more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables protein kinase C binding	IPI Inferred from Physical Interaction more info	PubMed
enables spectrin binding	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in Malpighian tubule morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in adherens junction organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in amnioserosa maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apical constriction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apical protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apical protein localization	IPI Inferred from Physical Interaction more info	PubMed
involved_in assembly of apicomedial cortex actomyosin	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell morphogenesis involved in Malpighian tubule morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye photoreceptor development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal closure, amnioserosa morphology change	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of apical/basal cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of polarity of embryonic epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of polarity of follicular epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in liquid clearance, open tracheal system	IGI Inferred from Genetic Interaction more info	PubMed
involved_in maintenance of apical/basal cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of Notch signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of organ growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of proteasomal ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nuclear chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in open tracheal system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in photoreceptor cell maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of hippo signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of hippo signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of imaginal disc growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rhabdomere development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rhabdomere morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in salivary gland morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in trachea development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in tube morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in wing disc dorsal/ventral pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in zonula adherens assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in zonula adherens maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apicolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apicolateral plasma membrane	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in photoreceptor connecting cilium	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in rhabdomere	IDA Inferred from Direct Assay more info	PubMed
part_of subapical complex	TAS Traceable Author Statement more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: crumbs

Names: CG6383-PA; CG6383-PB; CG6383-PC; CG6383-PD; crb-PA; crb-PB; crb-PC; crb-PD; fat rhabdomere; fat rhabdomeres; lethal (3) 07207

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

24295078..24314541

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001260355.2 → NP_001247284.1 crumbs, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001247284.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7R8

Conserved Domains (5) summary

cd00054
Location:792 → 828

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00110
Location:91 → 228

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...

pfam00008
Location:466 → 495

EGF; EGF-like domain

pfam00054
Location:1155 → 1290

Laminin_G_1; Laminin G domain

pfam02210
Location:1692 → 1828

Laminin_G_2; Laminin G domain
NM_001275980.1 → NP_001262909.1 crumbs, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262909.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KHS1

Conserved Domains (5) summary

cd00054
Location:959 → 995

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00110
Location:91 → 228

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...

pfam00008
Location:466 → 495

EGF; EGF-like domain

pfam00054
Location:1105 → 1240

Laminin_G_1; Laminin G domain

pfam02210
Location:1642 → 1778

Laminin_G_2; Laminin G domain
NM_001043286.2 → NP_001036751.1 crumbs, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036751.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P10040

Conserved Domains (5) summary

cd00054
Location:728 → 764

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00110
Location:91 → 228

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...

pfam00008
Location:466 → 495

EGF; EGF-like domain

pfam00054
Location:1091 → 1226

Laminin_G_1; Laminin G domain

pfam02210
Location:1628 → 1764

Laminin_G_2; Laminin G domain
NM_079756.3 → NP_524480.2 crumbs, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524480.2

Status: REVIEWED

UniProtKB/Swiss-Prot

P10040, Q0KI19, Q8MSX5, Q9VC97

Related

FBpp0083987

Conserved Domains (5) summary

cd00054
Location:685 → 721

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00110
Location:91 → 228

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...

pfam00008
Location:466 → 495

EGF; EGF-like domain

pfam00054
Location:1048 → 1183

Laminin_G_1; Laminin G domain

pfam02210
Location:1585 → 1721

Laminin_G_2; Laminin G domain