ago1 argonaute [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: ago1
Official Full Name: argonaute
Primary source: PomBase:SPCC736.11
Locus tag: SPOM_SPCC736.11
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: csp9
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Genomic context

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See ago1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 2

Sequence:: Chromosome: III; NC_003421.2 (334079..337070)

Chromosome III - NC_003421.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003421.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data indicate that surveillance of Argonaute-bound small RNAs by Cid14/Cid16 and the exosome protects the genome from uncontrolled RNAi and reveal a rapid RNAi-based adaptation to stress conditions.
Title: Tailing and degradation of Argonaute-bound small RNAs protect the genome from uncontrolled RNAi.
Survival in quiescence requires the euchromatic deployment of Clr4 by Ago1-associated small RNAs.
Title: Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
The Slicer activity of fission yeast Ago1 can be bypassed in sRNA-mediated H3K9 methylation, dsRNA synthesis, siRNA amplification, and gene silencing.
Title: Distinct Functions of Argonaute Slicer in siRNA Maturation and Heterochromatin Formation.
Studies indicate that S. pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1).
Title: RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe.
Data indicate that Argonaute, loaded with longer RNA precursors, recruits Triman (SPBC29A10.09c, 3'-5' Exonuclease) to generate dicer-independent priRNAs mature priRNAs and siRNAs.
Title: Argonaute and Triman generate dicer-independent priRNAs and mature siRNAs to initiate heterochromatin formation.
Nuclear accumulation of poly(A)(+) RNAs was detected in mutants of ago1(+) and ptr1(+), suggesting there is a functional link between the cell cycle checkpoint and RNAi-mediated RNA quality control.
Title: RNA interference regulates the cell cycle checkpoint through the RNA export factor, Ptr1, in fission yeast.
the amino terminus of Ago1 binds to proteins that function in cell cycle regulation including 14-3-3 proteins
Title: Interactions between the RNA interference effector protein Ago1 and 14-3-3 proteins: consequences for cell cycle progression.
Results describe a repetitive motif within Tas3, termed the 'Argonaute hook', that is conserved from yeast to humans and binds Ago1 and 2 through their PIWI domains in vitro and in vivo.
Title: A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain.
Argonaute requires the conserved aspartate-aspartate-histidine motif for heterochromatic silencing & for ribonuclease H-like cleavage (slicing) of target messages complementary to siRNA; spreading requires read-through transcription & slicing by Argonaute
Title: Argonaute slicing is required for heterochromatic silencing and spreading.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IDA Inferred from Direct Assay more info	PubMed
enables endoribonuclease activity, cleaving siRNA-paired mRNA	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-macromolecule adaptor activity	EXP Inferred from Experiment more info	PubMed
enables siRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in co-transcriptional gene silencing by RNA interference machinery	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of G0 to G1 transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear polyadenylation-dependent antisense transcript catabolic process	EXP Inferred from Experiment more info	PubMed
involved_in priRNA 3'-end processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulatory ncRNA-mediated heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in siRNA 3'-end processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in siRNA-mediated pericentric heterochromatin formation	EXP Inferred from Experiment more info	PubMed
involved_in siRNA-mediated pericentric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IDA Inferred from Direct Assay more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	EXP Inferred from Experiment more info	PubMed

Component	Evidence Code	Pubs
part_of ARC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RITS complex	EXP Inferred from Experiment more info	PubMed
part_of RITS complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in chromosome, telomeric repeat region	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	HDA more info	PubMed
is_active_in heterochromatin island	IDA Inferred from Direct Assay more info	PubMed
is_active_in mating-type region heterochromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in pericentric heterochromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in subtelomeric heterochromatin	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: argonaute

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003421.2 Reference assembly

Range

334079..337070

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001022775.3 → NP_587782.1 argonaute [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_587782.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

O74957

Conserved Domains (5) summary

PLN03202
Location:4 → 833

PLN03202; protein argonaute; Provisional

cd02846
Location:210 → 325

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:370 → 796

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:163 → 207

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:12 → 149

ArgoN; N-terminal domain of argonaute