GEO DataSets

(Submitter supplied) Commercial Atlantic halibut (Hippoglossus hippoglossus) farming is restricted by variable oocyte quality, slow growth, and early maturation of male fish. Maternally transferred components regulate early developmental processes; therefore, they have an effect on the future development of an embryo. We profiled components of the transcriptome involved in immune defence as well as germline and muscle development during early developmental stages: 8-cell embryos, germ ring stage, 10-somite stage, and hatched embryos using a 10k oligonucleotide array and quantitative real-time PCR to specifically identify transcripts useful as molecular markers of embryo quality.

Organism:

Hippoglossus hippoglossus

Type:

Expression profiling by array

Platform:

GPL19153

15 Samples

Download data: TXT

(Submitter supplied) Halibut fed two different diets containing either fishmeal(control) or 25-30% soybean meal for 20 days. Diets compared from fish (5) at day 1, day 10 and day 20 to follow the developement of the soybean-induced enteritis. All experimental samples run against universal RNA (cDNA prepared from 1 ug of a pooled universal RNA consisting of equal amounts of RNA from five developmental stages from hatching until post-metamorphosis). more...

Organism:

Hippoglossus hippoglossus

Type:

Expression profiling by array

Platform:

GPL6361

18 Samples

Download data: GPR

(Submitter supplied) Halibut switched to a microdiet and compared to control fed diet at 2, 10, 20 and 30 day post switch. 3 individual controls isolated and RNA pooled for a global control for each time point. Keywords: Diet comparison over a time course, for 1 diet compared to a reference.

Organism:

Hippoglossus hippoglossus

Type:

Expression profiling by array

Platform:

GPL6361

12 Samples

Download data: GPR

(Submitter supplied) An oligonucleotide microarray containing 50-mer oligonucleotides representing 9277 unique Atlantic halibut genes has been designed, printed and is currently being used for the study of gene expression in developing halibut. The oligonucleotides are based on all of the Atlantic halibut data available at the time of printing; these included ESTs and complete cDNAs derived from the Pleurogene sequencing project as well as sequences deposited in GenBank by other groups as of September 2006. more...

Organism:

Hippoglossus hippoglossus

Type:

Expression profiling by array

Platform:

GPL6361

15 Samples

Download data: CSV

(Submitter supplied) Agilent SureDesign, see https://earray.chem.agilent.com/

Organism:

Hippoglossus hippoglossus

1 Series

15 Samples

Download data: TXT

(Submitter supplied) Oligonucleotide DNA probes were designed from approximately 13000 available Atlantic halibut ESTs. The majority of the ESTs were derived by sequencing from the 5’ end of clones from 13 different normalized libraries from eight different tissues and five different developmental stages (Douglas et al., 2007). All EST data have been deposited in GenBank (accession numbers EB029285-EB041700 & EB080851-EB080975), and preliminary annotations are available on the Pleurogene website (www.pleurogene.ca). more...

Organism:

Hippoglossus hippoglossus

3 Series

45 Samples

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