GEO DataSets

(Submitter supplied) To investigate the interaction between two porcine isolates of P. multocida (PM2 for type D and PM7 for type A) with Aeromonas caviae by co-culturing P. multocida in the conditioned media prepared from A. caviae

Organism:

Pasteurella multocida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32586

12 Samples

Download data: TXT

(Submitter supplied) Pasteurella multocida is a Gram-negative capsulated bacterium responsible for a range of diseases that cause severe morbidity and mortality in livestock animals. The hyaluronic acid (HA) capsule produced by P. multocida serogroup A strains is a critical virulence factor. In this study, we utilised transposon-directed insertion site sequencing (TraDIS) to identify genes essential for in vitro growth of P. multocida, and combined TraDIS with discontinuous density gradients (TraDISort) to identify genes required for HA capsule production and regulation in this pathogen. more...

Organism:

Pasteurella multocida

Type:

Other

Platform:

GPL21442

10 Samples

Download data: CSV

(Submitter supplied) The Gram-negative pathogen Pasteurella multocida is responsible for many important animal diseases. While a number of P. multocida virulence factors have been identified, very little is known about how gene expression and protein production is regulated in this organism. One mechanism by which bacteria regulate transcript abundance and protein production is riboregulation, which involves the interaction of a small RNA (sRNA) with a target mRNA to alter transcript stability and/or translational efficiency. more...

Organism:

Pasteurella multocida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31945

9 Samples

Download data: CSV

(Submitter supplied) Objectives: To determine the transcripts that are differentially expressed in a hfq mutant. Hfq is an RNA chaperone that mediates many interactions between regultory RNAs and their mRNA targets. Analysis of the transcriptomes of the Pasteurella multocida wild-type strain and the Pasteurella multocida hfq mutant will allow for identification of genes controlled by hfq and the sRNAs with which it interacts.

Organism:

Pasteurella multocida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21442

6 Samples

Download data: CSV, FA, GFF

(Submitter supplied) P. multocida is the causative agent of a wide range of diseases of animals, including fowl cholera in birds. Fowl cholera isolates of P. multocida generally express a capsular polysaccharide composed of hyaluronic acid. There have been reports of spontaneous capsule loss in P. multocida fowl cholera-causing strains but the mechanism by which this occurs has not been determined. In this study, we identified three independent strains that had spontaneously lost the ability to produce capsular polysaccharide. more...

Organism:

Pasteurella multocida; Pasteurella multocida subsp. multocida str. Pm70

Type:

Expression profiling by array

Platform:

GPL8988

6 Samples

Download data: TXT

(Submitter supplied) We studied the effects of three classes of antibiotics (amoxicillin, chlortetracycline and enrofloxacin ) on P. multocida transcriptome using custom oligonucleotide microarrays from Nimblegen systems. All the 2015 genes of Pm70 were spotted on the array and hybridizations were carried out with RNA isolated from three independent cultures of Pm70 grown in the presence or absence of sub-minimum inhibitory (sub-MIC) doses of antibiotics. more...

Organism:

Pasteurella multocida subsp. multocida str. Pm70

Type:

Expression profiling by array

Platform:

GPL7307

12 Samples

Download data: PAIR

(Submitter supplied) Treatment of bacteria with antibiotics at or close to the inhibitory concentration leads to specific transcriptional responses often affecting target genes and targets pathways. A dataset of transcriptional profiles (compendium) induced by antibiotics with known mode-of-action (MoA) can be used to gain information on the putative MoA of novel substances with unknown MoAs. We used a Pasteurella multocida microarray to generate a compendium of transcriptional profiles and to obtain information on the putative MoA of a novel antibiotic compound. more...

Organism:

Pasteurella multocida subsp. multocida str. Pm70; Pasteurella multocida

Type:

Expression profiling by array

Platform:

GPL6291

72 Samples

Download data: CEL

(Submitter supplied) Probes were designed by Combimatrix as per the published PM70 genome sequence, with 2 probes per ORF >500bp and 1 probe per ORF under 500bp. Arrays were generated as per the Combimatrix protocols (http://www.combimatrix.com/tech_microarrays.htm)

Organism:

Pasteurella multocida subsp. multocida str. Pm70

1 Series

6 Samples

Download data: XML

(Submitter supplied) The custom oligonucleotide microarray consisting of all the 2015 annotated open reading frames (ORFs) of Pasteurella multocida strain Pm70 was constructed by NimbleGen Systems. The array design included 23 perfect match (PM) and mismatch (MM) probes per target gene, and each probe was spotted twice. Protocol: Maskless Array Synthesizer (MAS) technology

Organism:

Pasteurella multocida subsp. multocida str. Pm70

1 Series

12 Samples

Download data: NDF, NGD

(Submitter supplied) This GeneChip probe array is a powerful tool for monitoring trascriptional regulation and antimicrobial agent response of P. multocida. The array represents the annotated genome of P. multocida strain PM70 and includes 2,014 protein-coding sequences and a representative of the ribosomal RNA cluster. Protocol: see manufacturer's web site

Organism:

Pasteurella multocida subsp. multocida str. Pm70

1 Series

72 Samples

Download data