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Items: 1 to 20 of 1692

1.

Dual roles for a tick protein disulfide isomerase during the life cycle of the Lyme disease agent [I. scapularis]

(Submitter supplied) To determine whether the influences of Ixodes scapularis protein disulfide isomerase A6 (IsPDIA6) on Borrelia burgdorferi colonization is the result of physiological responses, we performed RNA-sequencing (RNA-seq) to compare the transcriptomes of control (GFP) and IsPDIA6-silenced ticks.
Organism:
Ixodes scapularis
Type:
Expression profiling by high throughput sequencing
Platform:
GPL29893
6 Samples
Download data: TXT
Series
Accession:
GSE273568
ID:
200273568
2.

Transcriptome profiling of cell lines from Ixodes scapularis

(Submitter supplied) The goal of this study was to identify and compare baseline mRNA expression levels for a set of Ixodes scapularis cell lines originally reported in Munderloh et al. 1994 Journal of Parasitology 80(4):533-43. Three unmodified I. scapularis cell lines were included in the study: ISE6, IDE2, and ISE18. In addition, one cell population modified by insertion of a transgenic cassette using Sleeping Beauty transposition was also included: ISE18SB. more...
Organism:
Ixodes scapularis
Type:
Expression profiling by high throughput sequencing
Platform:
GPL34584
14 Samples
Download data: CSV
Series
Accession:
GSE269712
ID:
200269712
3.

Adiponectin inhibits acquisition of the Lyme disease agent by ticks [Tick]

(Submitter supplied) Tick gut and salivary gland global gene expression change after feeding on adiponectin wildtype and knockout mice
Organism:
Ixodes scapularis
Type:
Expression profiling by high throughput sequencing
Platform:
GPL29893
4 Samples
Download data: TXT
Series
Accession:
GSE233660
ID:
200233660
4.

Subolesin knockdown in tick cells provides insights into vaccine protective mechanisms

(Submitter supplied) Ticks, as obligate blood-feeding arthropod vectors of pathogenic viruses, bacteria, protozoa and helminths, are responsible for prevalent tick-borne diseases (TBDs) worldwide. This arthropod constitutes the second most common that transmit pathogens among humans, after mosquitoes, and the first vector in domestic animals. Vaccines constitute the safest and more effective approach to control tick and TBDs, but this is in constant research to identify new antigens and improve vaccines formulations. more...
Organism:
Ixodes scapularis
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21603
4 Samples
Download data: XLSX
Series
Accession:
GSE236747
ID:
200236747
5.

Fenbutatin oxide resistance in the spider mite Tetranychus urticae

(Submitter supplied) Gene expression differences between MR-VL_FBO, a T. urticae strain highly resistant to fenbutatin oxide, and KOP8, a T. urticae strain susceptible to fenbutatin oxide, were determined.
Organism:
Tetranychus urticae
Type:
Expression profiling by array
Platform:
GPL16890
4 Samples
Download data: TXT
Series
Accession:
GSE168504
ID:
200168504
6.

Induction and suppression of Haemaphysalis longicornis ticks antiviral RNA interference responses by arthropod-borne RNA viruses (RNA-Seq)

(Submitter supplied) Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne bunyavirus that causes severe clinical symptoms and mortality in humans. Haemaphysalis longicornis tick has been identified as the competent vector for SFTSV transmission. Although antiviral RNA interference (RNAi) in insects has been well documented, the degree to which RNAi contributes to antiviral defense in ticks is still largely elusive. more...
Organism:
Haemaphysalis longicornis
Type:
Expression profiling by high throughput sequencing
Platform:
GPL29627
9 Samples
Download data: FASTA, TXT
Series
Accession:
GSE165139
ID:
200165139
7.

Venomous gland transcriptome and venom proteomic analysis of the scorpion Androctonus amoreuxi reveal new peptides with anti-SARS-CoV-2 activity

(Submitter supplied) The recent COVID-19 pandemic shows the critical need for novel broad spectrum antiviral agents. Scorpion venoms are known to contain highly bioactive peptides, several of which have demonstrated strong antiviral activity against a range of viruses. We have generated the first annotated reference transcriptome for the Androctonus amoreuxi venom gland and used high performance liquid chromatography, transcriptome mining, circular dichroism and mass spectrometric analysis to purify and characterize twelve previously undescribed venom peptides. more...
Organism:
Androctonus amoreuxi
Type:
Expression profiling by high throughput sequencing
Platform:
GPL32983
1 Sample
Download data: FASTA, XLSX
Series
Accession:
GSE221799
ID:
200221799
8.

Rhipicephalus microplushemocyte innate cellular and humoral immune responses to babesialinfection

(Submitter supplied) To understand the effect of Babesia infection on Rhipicephalus microplus hemocyte gene expression, we performed high throughput RNA-sequencing using samples collected from Rhipicephalus microplus uninfected tick hemolymph and infected with either Babesia bigemina or Babesia bovis. We evaluated gene expression differences that may be attributed to tick immune defense to babesial infection.
Organism:
Rhipicephalus microplus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL33773
9 Samples
Download data: XLSX
Series
Accession:
GSE243493
ID:
200243493
9.

Whole genome duplication in arachnids - what happened next?

(Submitter supplied) About 430 million years ago spiders and scorpions evolved from a common ancestor that had experienced a whole genome duplication (WGD) The genetic remnants of this WGD event (genes called ohnologs) can still be found in the genome of the approximately 45,000 species of these animals alive today and these ohnologs may have contributed to their adaptation and diversification. Interestingly, the WGD in arachnids like scorpions and spiders was contemporary with independent WGDs in vertebrates. more...
Organism:
Segestria senoculata
Type:
Expression profiling by high throughput sequencing
Platform:
GPL33691
1 Sample
Download data: FASTA, TXT
Series
Accession:
GSE241073
ID:
200241073
10.

Single-cell transcriptomics reveals the brain evolution of web-building spiders

(Submitter supplied) Spiders are renowned for their efficient capture of flying insects using intricate aerial webs. How the spider nervous systems evolved to cope with this specialized hunting strategy and various environmental clues in an aerial space remains unknown. Here, we report a brain cell atlas of >30,000 single-cell transcriptomes from a web-building spider (Hylyphantes graminicola). Our analysis revealed the preservation of ancestral neuron types in spiders, including the potential coexistence of noradrenergic and octopaminergic neurons, and many peptidergic neuronal types that are lost in insects. more...
Organism:
Hylyphantes graminicola
Type:
Expression profiling by high throughput sequencing
Platform:
GPL33712
5 Samples
Download data: MTX, TSV
Series
Accession:
GSE241696
ID:
200241696
11.

Rational identification of novel drug targets and vaccine candidates against the cattle tick, Rhipicephalus microplus

(Submitter supplied) Control of complex parasites via vaccination remains challenging, with the current combination of vaccines and small drugs remaining the choice of control strategy. The development of new vaccines against complex endo- and ectoparasites remains challenging and more studies are providing evidence that multicomponent vaccines will be needed for the development of protective vaccines against parasites. more...
Organism:
Rhipicephalus microplus
Type:
Expression profiling by array
Platform:
GPL32975
6 Samples
Download data: GPR
Series
Accession:
GSE221646
ID:
200221646
12.

Transcriptome changes reveal the toxic mechanism of cadmium and lead combined exposure on silk production and web-weaving behavior of spider A. ventricosus

(Submitter supplied) The goals of this study are to compare NGS-derived transcriptome of ampullate gland and cerebral ganglia under single and combined exposure to cadmium (Cd) and lead (Pb). The upregulated ampullate spidroin genes, downregulated protein anabolic genes, upregulated Far genes, and downregulated TUBA genes exhibit promising potential as biomarkers for silk protein synthesis and cellular degeneration, respectively. more...
Organism:
Araneus ventricosus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL33678
24 Samples
Download data: FASTA, TXT
Series
Accession:
GSE240836
ID:
200240836
13.

Identification and characterization of striking multiple-insecticide resistance in a Tetranychus urticae field population from Greece.

(Submitter supplied) We identified and characterized a striking case of multiple acaricide resistance in a field population of T. urticae. Exceptionally strong resistance phenotypes, with accumulation of multiple resistance mutations and over-expression of P450s and other detoxification genes in the same field population is reported.
Organism:
Tetranychus urticae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL28937
12 Samples
Download data: TXT
Series
Accession:
GSE155156
ID:
200155156
14.

Expression quantitative trait locus (eQTL) mapping with a cross between Tetranychus urticae inbred strains MR-VPi and ROS-ITi

(Submitter supplied) We performed expression quantitative trait locus (eQTL) mapping between two strains of Tetranychus urticae (the two-spotted spider mite), a generalist herbivore known for its rapid evolution of acaricide resistance. For parents, we used the inbred strain MR-VPi that is highly resistant to multiple acaricides in different classes, and the inbred strain ROS-ITi that is comparatively susceptible to many acaricides. more...
Organism:
Tetranychus urticae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL28937
506 Samples
Download data: TXT
Series
Accession:
GSE221677
ID:
200221677
15.

Regulation of chromatin accessibility in early embryos of the common house spider Parasteatoda tepidariorum

(Submitter supplied) We identified a lineage-specific GATA-like gene named fuchi nashi (fuchi) as a gene required for establishing a germ disc in the early embryo of the common house spider Parasteatoda tepidariorum. To investigate possible involvement of the early fuchi activity in regulation of chromatin accessibility, we used an assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq). Whole embryos at stage 3 that were untreated (wild-type) or treated for fuchi or hedgehog (hh) parental RNAi (pRNAi), as well as wild-type whole embryos at stages 1 and 2, were analyzed by ATAC-seq. more...
Organism:
Parasteatoda tepidariorum
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL31240
10 Samples
Download data: BW, NARROWPEAK
Series
Accession:
GSE193870
ID:
200193870
16.

Identification of genes whose expression is affected by fuchi nashi pRNAi in early embryos of the spider Parasteatoda tepidariorum

(Submitter supplied) We identified a GATA-like gene named fuchi nashi (fuchi) as a gene required for establishing a germ disc in the early embryo of the spider Parasteatoda tepidariorum. fuchi transcript is initially expressed in cells at both polar regions of the embryo and later in endodermal and extraembryonic cells. To genome-widely identify genes whose expressions are regulated by fuchi, we conducted RNA-sequencing of whole embryos at three different early stages (stages 2 and 3 and early stage 5) that were untreated (control) or treated for fuchi parental RNA interference (pRNAi).
Organism:
Parasteatoda tepidariorum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24841
12 Samples
Download data: BW, TXT
Series
Accession:
GSE193650
ID:
200193650
17.

Genome-wide identification of candidate genes whose transcripts are locally expressed in early embryos of the spider Parasteatoda tepidariorum

(Submitter supplied) The early embryo of the spider Parasteatoda tepidariorum initially shows spherical symmetry in morphology. Following a symmetry breaking event, it forms a radially symmetric germ disc in the hemisphere of the egg. In this work, we conducted RNA-sequencing of cells isolated from small different regions of radially symmetric forming/formed germ discs, aming to identify candidate genes whose transcripts are locally expressed in early spider embryos.
Organism:
Parasteatoda tepidariorum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24841
16 Samples
Download data: BW, TXT
Series
Accession:
GSE193511
ID:
200193511
18.

Single-cell and single-nucleus transcriptome analysis of pattern formation in the early embryo of the spider Parasteatoda tepidariorum

(Submitter supplied) In a cellular field of the early spider embryo, Hedgehog signaling operates to specify a “fuzzy” French-flag-like pattern along the primary axis. We applied single-cell and single-nucleus RNA sequencing to the early spider embryo. We confirmed that these techniques successfully detected three cell population corresponding to germ layers and some known cell types. We showed that the data had sufficient information for reconstruction of a correct global polarity of the presumptive ectoderm.
Organism:
Parasteatoda tepidariorum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL31240
3 Samples
Download data: CSV, TXT
Series
Accession:
GSE201705
ID:
200201705
19.

Agelena koreana whole body and venom gland transcriptome

(Submitter supplied) Agelena koreana is indigenous spider in South Korea that lives on piles of trees building webs. RNA-sequencing was performed for venom gland tissue and whole body except venom gland.
Organism:
Agelena koreana
Type:
Expression profiling by high throughput sequencing
Platform:
GPL31963
6 Samples
Download data: BED, FASTA, GFF3, TSV, TXT
Series
Accession:
GSE197102
ID:
200197102
20.

Transcriptome responses to sublethal concentration of abamectin in Tetranychus truncatus analyzed by deep-sequencing

(Submitter supplied) Purpose: In order to clarify the molecular response mechanism of Tetranychus truncatus to sublethal concentrations of abamectin. Methods: We sequenced the transcriptome of Tetranychus truncatus treated with sublethal concentration of abamectin and control. Results: we provide a comprehensive transcriptome resource for Tetranychus truncatus derived from treating with sublethal concentration abamectin and control. more...
Organism:
Tetranychus truncatus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL25155
6 Samples
Download data: FASTA, TXT
Series
Accession:
GSE115441
ID:
200115441
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