GEO DataSets

(Submitter supplied) In this study we compare logarithmically grown Burkholderia thailadensis wildtype to a transposon mutant that is disrupted in gshA1, or gshA2, which are involved in glutathione biosynthesis or egtB, involved in ergothioneine biosynthesis using RNASeq to identify novel pathways where glutathione or ergothioneine may be involved.

Organism:

Burkholderia thailandensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33677

8 Samples

Download data: CSV, XLSX

(Submitter supplied) Introduction. Burkholderia thailandensis is a clinically rare opportunistic pathogen in the genus Burkholderia, and the genomic features and virulence characteristics of B. thailandensis strains that cause human infection remain unclear.Gap Statement. B. thailandensis strains with different virulence induce different host innate immune responses in vitro.Aim. This work aimed to understand the sequence diversity, phylogenetic relationship, and virulence of B. more...

Organism:

Burkholderia thailandensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23641

6 Samples

Download data: TXT

(Submitter supplied) Persister cells are genetically identical variants in a bacterial population that have phenotypically modified their physiology to survive environmental stress. . To better define general persistence mechanisms that can be targeted to develop anti-persistence therapeutics, we performed transcriptomics analyses of Burkholderia thailandensis enriched for persisters using three methods: flow sorting by low proton motive force, meropenem treatment, and culture aging. more...

Organism:

Burkholderia thailandensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21997

18 Samples

Download data: CSV, TSV

(Submitter supplied) The interactions between Gram-negative respiratory pathogens and the host environment at the site of infection largely unknown. Pulmonary surfactant serves as an initial point of contact for inhaled bacteria entering the lung and is thought to contain molecular cues that aid colonization and pathogenesis. To gain insight into this ecological transition, we characterized the transcriptional responses of Pseudomonas aeruginosa PA14, Burkholderia thailandensis E264, Klebsiella pneumoniae MGH 78578, and Stenotrophomonas maltophilia K279A exposed to purified pulmonary surfactant (Survanta) through microarrays. more...

Organism:

Klebsiella pneumoniae MGH 78578; Pseudomonas aeruginosa PA14; Burkholderia thailandensis E264; Stenotrophomonas maltophilia K279a

Type:

Expression profiling by array

Platform:

GPL24615

8 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Comparison of transcriptomes of wild-type cells and cells deleted for MftR as well as both strain grown in absence and presence of 5 mM urate

Organism:

Burkholderia thailandensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23641

12 Samples

Download data: XLSX

(Submitter supplied) In prokaryotes and eukaryotes, cell-cell communication and recognition of self are critical to coordinate multicellular functions. While kin and kind discrimination are increasingly appreciated to shape naturally occurring microbe populations, the underlying mechanisms that govern these interbacterial interactions are insufficiently understood. Here we identify a mechanism of interbacterial signal transduction that is mediated by contact-dependent growth inhibition (CDI) system proteins. more...

Organism:

Burkholderia thailandensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21997

9 Samples

Download data: XLSX

(Submitter supplied) Burkholderia thailandensis is a soil-dwelling bacterium that shares many metabolic pathways with the ecologically similar, but evolutionarily distant, Pseudomonas aeruginosa. Among the diverse nutrients it can utilize is choline, which can be converted into the osmoprotectant glycine betaine and further catabolized as a source of carbon and nitrogen, similar to P. aeruginosa. Orthologs of genes in the choline catabolic pathway in these two bacteria showed distinct differences in gene arrangement as well as an additional orthologous transcriptional regulator in B. more...

Organism:

Burkholderia thailandensis E264

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21888

16 Samples

Download data: XLSX

(Submitter supplied) Bacterial small RNAs (sRNAs) regulate gene expression by base-pairing with downstream target mRNAs to attenuate translation of mRNA into protein at the post-transcriptional level. In response to specific environmental changes, sRNAs can modulate the expression levels of target genes, thus enabling adaptation of cellular physiology. We profiled sRNA expression in the Gram-negative bacteria Burkholderia thailandensis cultured under 54 distinct growth conditions using a Burkholderia-specific microarray that contains probe sets to all intergenic regions (IGRs) greater than 90 bases. more...

Organism:

Burkholderia thailandensis E264

Type:

Expression profiling by array

Platform:

GPL18529

162 Samples

Download data: CEL

(Submitter supplied) An important, but rarely performed, test of Koch’s molecular postulates involves evaluating the capacity of candidate virulence genes to confer pathogenicity in otherwise non-virulent species. Unbiased genomic surveys of avirulent natural isolates might reveal rare variants possessing specific virulence features, which might prove useful in testing their functional sufficiency. Using a custom pan-genome array, we analyzed a panel of avirulent Burkholderia thailandensis (Bt) isolates related to Burkholderia pseudomallei (Bp), the causative agent of the often fatal human and animal disease melioidosis. more...

Organism:

Burkholderiales; Burkholderia thailandensis

Type:

Genome variation profiling by genome tiling array

Platform:

GPL9490

49 Samples

Download data: PAIR

(Submitter supplied) Total transcript amplification (TTA) from single eukaryotic cells for transcriptome analysis is established, but TTA from a single prokaryotic cell presents additional challenges with much less starting material and lack of poly(A)-tails. We described, here, a novel method for single bacterium TTA, using a Burkholderia thailandensis model exposed to subinhibitory concentration of the antibacterial agent, glyphosate. more...

Organism:

Burkholderia thailandensis

Type:

Expression profiling by array

Platform:

GPL7113

3 Samples

Download data: TXT

(Submitter supplied) see manufacturer's web site

Organism:

Burkholderia thailandensis E264; Stenotrophomonas maltophilia K279a; Pseudomonas aeruginosa PA14; Klebsiella pneumoniae MGH 78578

1 Series

8 Samples

Download data: CDF, CSV, GIN, TXT

(Submitter supplied) A custom Affymetrix microarray was designed using Burkholderia thailandensis E264 gene annotations from Pathema and included 8711 probe sets targeting 5557 protein-coding genes and 2908 intergenic regions (IGRs) longer than 90 bases on the plus strand Protocol: in situ oligonucleotide synthesis (see manufacturer's web site)

Organism:

Burkholderia thailandensis E264

1 Series

162 Samples

Download data: CDF, GFF3, GIN, SIF, TXT

(Submitter supplied) Commercially synthesized oligonucleotides were diluted in pronto buffer (corning) and printed on corning UltraGAPS slides. The array consists of 19,200 spots arranged in 48 grids (4 columns and 12 rows). Each grid consists of a 20x20 set of spots. The 5210 ORFs represented on the array were spotted in triplicate and the remaining 3570 spots are blank.

Organism:

Burkholderia thailandensis

1 Series

3 Samples

Download data