GEO DataSets

(Submitter supplied) A shotgun metagenome microarray was created and used to investigate gene transcription during vinyl chloride (VC) dechlorination by a microbial enrichment culture called KB1. The array was constructed by spotting genomic fragments amplified from short-insert libraries of KB1 metagenomic DNA. Subsequently, the microarrays were interrogated with RNA extracted from KB1 during VC dechlorination (VC+methanol), and in the absence of VC (methanol-only). more...

Organism:

dechlorinating enrichment culture KB-1

Type:

Expression profiling by array

Platform:

GPL9981

6 Samples

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(Submitter supplied) KB1 mixed microbial consortium. Composition of community as estimated by 16S qPCR: Dehalococcoides:48.1-70.2% Chlorobi SJA-28:6-14.9% Acetobacterium:2.65-12% Sporomusa:1.2-11.45% Spirochaete SA-8 :2.8-8.3% Methanomicrobiales:3.5-5.8% OP5:2.5-5% Geobacter:3-4.4% Methanosarcina:0.1-1% Methanosaeta:0-0% Bacteroidales 1:0-0.8% Syntrophus:0-0.03% Syntrophobacter:0-0.2% Sulfurospirillum:0-0.002% Methanomethylvorans:0-0.04% Protocol: 1 - gDNA was extracted from the mixed culture KB1, 1-3 kb fragments were eluted and purified from 1% agarose gel 2 - The fragments were then blunt-end repaired and dephosphorylated before undergoing blunt-end cloning. more...

Organism:

dechlorinating enrichment culture KB-1

1 Series

6 Samples

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