GEO DataSets

(Submitter supplied) Biofilm lifestyle is critical for bacterial pathogens to colonize and protect themselves from host immunity and antimicrobial chemicals in plants and animals. The formation and regulation mechanism of phytobacterial biofilm are still obscure. Here, we found that Ralstonia solanacearum Resistance to ultraviolet C (RuvC) is highly abundant in biofilm and positively regulates pathogenicity by governing systemic movement in tomato xylem. more...

Organism:

Ralstonia solanacearum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34644

6 Samples

Download data: TXT

(Submitter supplied) Plant pathogens require lethal virulence factors, susceptible hosts, and optimal environmental conditions for disease establishment. High soil salinity, exacerbated by climate change, significantly impacts agro-biological ecosystems. However, the overall interactions between plant pathogens and salt stress are not fully characterized or understood. This study examines the effects of salt stress on representative plant pathogens: Burkholderia gladioli, Pectobacterium carotovorum subsp. more...

Organism:

Ralstonia solanacearum; Burkholderia gladioli; Pectobacterium carotovorum subsp. carotovorum

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL34740 GPL29295 GPL34741

18 Samples

Download data: TXT

(Submitter supplied) Bacterial pathogens exhibit a remarkable ability to persist and thrive in diverse ecological niches. Understanding the mechanisms enabling their transition between environments is crucial to control dissemination and potential disease outbreaks. Here, we use Ralstonia solanacearum, the causing agent of the bacterial wilt disease, as a model to investigate the bacterial transcriptomic adaptation to two environments, water and soil, and compare it with the well-studied in planta gene expression. more...

Organism:

Ralstonia solanacearum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33632

6 Samples

Download data: CSV, XLSX

(Submitter supplied) Ralstonia solanacerum causes bacterial wilt disease on many important host crops, including tomato and potato. R. solanacearum cells enter a host from soil or infested water through the roots, then multiply and spread in the water-transporting xylem vessels. Despite the low nutrient and oxygen content of xylem sap, R. solanacearum grows extremely well inside the host, using denitrification to respire in this hypoxic environment. more...

Organism:

Ralstonia solanacearum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29295

9 Samples

Download data: TXT

(Submitter supplied) Ralstonia solancearum causes bacterial wilt disease on diverse plant hosts. R. solanacearum cells enter a host from soil or infested water through the roots, then multiply and spread in the water-transporting xylem vessels. Despite the low nutrient content of xylem sap, R. solanacearum grows extremely well inside the host, using denitrification to respire in this hypoxic environment. R. solanacearum growth in planta also depends on the successful deployment of protein effectors into host cells using a Type III Secretion System (T3SS). more...

Organism:

Ralstonia solanacearum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29295

24 Samples

Download data: TXT

(Submitter supplied) Purpose: To comprehensively identify traits controlled by the PhcA quorum sensing system, and to better understand how this regulator affects pathogen behavior in its natural host environment Method: 21 day-old tomato plants were inoculated with approximately 2000 cells of either wild-type Rs strain GMI1000 or ΔphcA through a cut petiole. After 72 h in a 28°C growth chamber (12h day/night cycle), 0.1 g of stem tissue spanning the petiole was harvested and transferred immediately to a tube containing 900 µl of pre-chilled transcription stop solution (5% [vol/vol] water-saturated phenol in ethanol) and ground using a Powerlyzer. more...

Organism:

Ralstonia solanacearum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23347

6 Samples

Download data: XLSX

(Submitter supplied) The aim of the project is to decipher the role of DNA methylation in the plant pathogenic bacteria Ralstonia solanacearum during host adaptation. As a first step, we present here the DNA methylation profile of the GMI1000 reference strain.

Organism:

Ralstonia solanacearum

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL22867

1 Sample

Download data: CSV, GFF

(Submitter supplied) Examination of modified bases on the genome of Ralstonia solanacearum UY031

Organism:

Ralstonia solanacearum

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL22867

1 Sample

Download data: CSV, GFF

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Ralstonia pseudosolanacearum GMI1000; Ralstonia solanacearum UW551

Type:

Expression profiling by array; Expression profiling by genome tiling array

Platforms:

GPL14879 GPL14878

32 Samples

Download data: TXT

(Submitter supplied) Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain UW551 at 20°C and 28°C in culture and in planta. The temperatel strain UW551 can wilt and cause full-blown disease on tomato plants at 28°C as well as at 20°C.

Organism:

Ralstonia solanacearum UW551

Type:

Expression profiling by array; Expression profiling by genome tiling array

Platform:

GPL14879

16 Samples

Download data: TXT

(Submitter supplied) Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain GMI1000 at 20°C and 28°C in culture and in planta. The tropical strain GMI1000 cannot wilt tomato plants at 20°C although it can cause full-blown disease at 28°C.

Organism:

Ralstonia pseudosolanacearum GMI1000

Type:

Expression profiling by array; Expression profiling by genome tiling array

Platform:

GPL14878

16 Samples

Download data: TXT

(Submitter supplied) fRSL1 is a jumbo myovirus stably and lytically infecting the phytopathogenic bacterium Ralstonia solanacearum. In this study, we investigate the infection cycle of fRSL1 and provide a genomic, proteomic and transcriptomic view of this phage. Its 231-kbp genome sequence showed many genes lacking detectable homologs in the current databases and was vastly different from previously studied phage genomes. more...

Organism:

Ralstonia solanacearum; Ralstonia phage phiRSL1

Type:

Expression profiling by array

Platform:

GPL10654

5 Samples

Download data: TXT

(Submitter supplied) NimbleGen design name Ralstonia solanacearum UW551, NimbleGen design ID 13052. An expression design for 4318 genes from Ralstonia solanacearum strain UW551 with 2 to 6, 40-70 mer probes per gene, with two-fold technical redundancy.The arrays also contain probes for intergenic regions with no technical replicates. The design includes random GC and other control probes. Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. more...

Organism:

Ralstonia solanacearum UW551

2 Series

16 Samples

Download data: NDF, NGD

(Submitter supplied) NimbleGen design name Ralstonia solanacearum GMI1000, NimbleGen design ID 13605. An expression design for 5061 genes from Ralstonia solanacearum strain GMI1000 with 2 to 6, 40-70 mer probes per gene, with two-fold technical redundancy.The arrays also contain probes for intergenic regions with no technical replicates. The design includes random GC and other control probes. Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. more...

Organism:

Ralstonia pseudosolanacearum GMI1000

2 Series

16 Samples

Download data: NDF, NGD