GEO DataSets

(Submitter supplied) Plants are targets of volatile organic compounds (VOCs) released as a part of plant-plant communication, within-plant self-signaling and plant-microbe interactions. Therefore, understanding VOC perception and downstream signaling is vital for unraveling the mechanisms behind information exchange in plants, which remain largely unexplored. Using the hormone-like function of volatile terpenoids in reproductive organ development as a system with a visual marker for communication, we demonstrated that among the four petunia karrikin-insensitive receptors, PhKAI2ia stereo-specifically perceives the (-)-germacrene D signal, triggering a karrikin-like signaling cascade. more...

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21338

12 Samples

Download data: TXT

(Submitter supplied) To better understand how diurnal transcriptional regulation contributes to day/night cycles of volatile emission from the petunia flower, cross-linked chromatin from corolla in the morning (7AM) and evening (7PM) was immunoprecipitated with antibodies against 4 histone marks associated with trancriptional activity to determine islands enriched for the marks in morning and evening.

Organism:

Petunia x hybrida

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL30987

22 Samples

Download data: TXT

(Submitter supplied) To better understand how diurnal transcriptional regulation contributes to day/night cycles of volatile emission from the petunia flower, RNA extracted from corolla in the morning (7AM) and evening (7PM) was sequenced to determine genes differentially expressed at the two time points.

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21338

6 Samples

Download data: TXT

(Submitter supplied) Shoot branching of flowering plants exhibits phenotypic plasticity and variability. This plasticity is determined by the activity of axillary meristems, which in turn is influenced by endogenous and exogenous cues such as nutrients and light. In many species, not all buds on the main shoot develop into branches despite favorable growing conditions. In petunia, basal buds (buds 1-3) typically do not grow out to form branches, while more apical buds (buds 6 and 7) are competent to grow. more...

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30987

48 Samples

Download data: CSV

(Submitter supplied) Petunia floral scent production and emission is highly regulated, with a major role for the transcription factor ODORANT1 (ODO1) in directing activation of volatile biosynthesis. Using ChIP-seq of tagged ODO1 protein from petunia flowers, and RNA-seq of wild-type and odo1i RNAi flowers, the ODORANT1-regulated gene network of petunia is described, which extends to branches involved in phenylpropanoid intermediate production and S-adenosyl-methionine biosynthesis to potentiate production and emission of volatiles. more...

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL30987 GPL20694

8 Samples

Download data: TXT

(Submitter supplied) The transcriptional response of the overexpressor of synthetic PhADT, generated through the site-directed mutagenesis of native PhADT(R353A) was compared with wild-type PhADT expressed in the Petunia flowers.

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21338

9 Samples

Download data: FASTA, TXT

(Submitter supplied) N1-methyladenosine is a unique base methylation because it blocks Watson-Crick base paring and introduces a positive charge. Previous studies showed that m1A is prevalent in yeast and mammals mRNA and has a functional role in promoting translation of methylated mRNA. However, little is known about its abundance, topology and dynamics in plant mRNA. In this study, dot blotting and LC–MS/MS analyses reveal a dynamic pattern of m1A mRNA modification in various tissues and at different developmental stages in petunia (Petunia hybrida). more...

Organism:

Petunia x hybrida

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL27424

10 Samples

Download data: BED

(Submitter supplied) White areas of star-type bicolor petals of petunia (Petunia hybrida) are caused by post-transcriptional gene silencing (PTGS) of the key enzyme of anthocyanin biosynthesis. We observed blotched flowers and vein-clearing symptom in leaves in 3- to 4-month-old petunia plants. In order to determine the cause of blotched flowers, we examined an endogenous pararetrovirus, petunia vein clearing virus (PVCV), because this virus causes vein-clearing symptom and may have a suppressor of PTGS. more...

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21338

6 Samples

Download data: TXT

(Submitter supplied) Aim: compare the transcriptionnal responses induced by mycorrhiza in wild type and GRAS transcription factor RAM1 mutant ram1-2 in roots. Results: 650 genes were upregulated in mycorrhized WT (fold change >5; FDR<0,05, experiment difference>10) and 201 in the ram1 mutant. 182 genes were induced in both WT and ram1. 468 and 19 genes were induced only in WT or ram1-2 respectively. 91 genes were downregulated in mycorrhized WT (fold change <5; FDR<0,05, experiment difference>10) and 51 in ram1-2. more...

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20694

12 Samples

Download data: XLS

(Submitter supplied) One of the primary objectives of plant biotechnology is to increase resistance to abiotic stresses, such as salinity. Salinity is a major abiotic stress and increasing crop resistant to salt continues to the present day as a major challenge. Salt stress disturbs cellular environment leading to protein misfolding, affecting normal plant growth and causing agricultural losses worldwide. The advent of state-of-the-art technologies such as high throughput mRNA sequencing (RNA-Seq) has revolutionized whole-transcriptome analysis by allowing, with high precision, to measure changes in gene expression. more...

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21338

48 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Petunia x hybrida; Vitis vinifera

Type:

Expression profiling by array

Platforms:

GPL22427 GPL22428

12 Samples

Download data: PAIR, TXT

(Submitter supplied) The WRKY family represents a plant-specific class of zinc-finger transcription factors (TF) well known for regulating abiotic and biotic stress tolerance and also for their emergent role in the control of various developmental and physiological processes (Rushton et al. 2010, Schluttenhofer et al. 2015). The well-characterized AtTTG2, PhPH3 and BnTTG2 are ortholog WRKY proteins with interchangeable functions ascribed to an emergent clade whose regulatory functions are mainly associated with vacuolar metabolism (Li et al. more...

Organism:

Petunia x hybrida

Type:

Expression profiling by array

Platform:

GPL22428

6 Samples

Download data: PAIR, TXT

(Submitter supplied) Salinity and drought stress are the primary cause of crop losses worldwide. In sodic saline soils sodium chloride (NaCl) disrupts normal plant growth and development. The complex interactions of plant systems with abiotic stress have made RNA sequencing a more holistic and appealing approach to study transcriptome level responses in a single cell and/or tissue. In this work, we determined the Petunia transcriptome response to NaCl stress by sequencing leaf samples and assembling 196 million Illumina reads with Trinity software. more...

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21338

32 Samples

Download data: CSV, FA, TXT

(Submitter supplied) To accurately detect and quantify low abundant transcripts no represented in current EST databases we generated an RNA-seq dataset based on RNA isolated from Petunia hybrida corolla tissue harvested at 8 PM at two developmental stages, day -1 (bud) and day 2 postanthesis (the tissues containing the lowest and highest levels of phenylalanine, respectively).

Organism:

Petunia x hybrida

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20694

6 Samples

Download data: FASTA, TXT

(Submitter supplied) Investigation of gene expression level changes in Petunia hybrida seedlings subjected to cold at 2°C for 0.5 h, 2 h, 24 h and 5 d, compared to the CK.

Organism:

Petunia x hybrida

Type:

Expression profiling by array

Platform:

GPL18705

15 Samples

Download data: PAIR

(Submitter supplied) In order to identify genes specifically induced during various developmental stages of Adventitious Root (AR) formation (described in Ahkami et al. 2009) in leafy cuttings of Petunia hybrida (line W115) and to describe the series of physiological processes during adventitious rooting, a microarray-based transcriptome analysis in the stem base of the cuttings was conducted. The microarray was described by Breuillin et al. more...

Organism:

Petunia x hybrida

Type:

Expression profiling by array

Platform:

GPL18705

28 Samples

Download data: TXT, XLS

(Submitter supplied) Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from Petunia x hybrida tissues (including leaves and flowers). more...

Organism:

Petunia x hybrida

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9397

1 Sample

Download data: TXT

(Submitter supplied) To obtain insights about the roles of VvMYB5a and VvMYB5b, here we perform complementation analyses using petunia regulatory mutants impaired in pigment accumulation in flower epidermis, proven to be a valid tool for gene functional studies.

Organism:

Petunia x hybrida; Petunia axillaris

Type:

Expression profiling by array

Platform:

GPL13298

12 Samples

Download data: TXT

(Submitter supplied) Background: Introduction of a transgene that transcribes RNA homologous to an endogenous gene in the plant genome can induce silencing of both genes, a phenomenon termed cosuppression. Cosuppression was first discovered in transgenic petunia plants transformed with the CHS-A gene encoding chalcone synthase, in which nonpigmented sectors in flowers or completely white flowers are produced. Some of the flower-color patterns observed in transgenic petunias having CHS-A cosuppression resemble those in existing nontransgenic varieties. more...

Organism:

Petunia x hybrida

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16386

4 Samples

Download data: TXT

(Submitter supplied) Canola (oilseed rape, Brassica napus L.), is susceptible to infection by the biotrophic protist Plasmodiophora brassicae, the causal agent of clubroot. To understand the roles of microRNAs (miRNAs) during the post-transcriptional regulation of disease initiation and progression, we have characterized the changes in miRNA expression profiles in canola roots during clubroot disease development and have compared these to uninfected roots. more...

Organism:

Pinus taeda; Liriodendron tulipifera; Phaseolus vulgaris; Ipomoea batatas; Lactuca sativa; Oryza rufipogon; Oryza nivara; Sorghum bicolor; Rorippa indica; Prunus salicina; Populus tremula; Malcolmia maritima; Physcomitrium patens; Persea americana; Populus trichocarpa x Populus deltoides; Malus domestica; Nicotiana sylvestris; Nicotiana tabacum; Petunia x hybrida; Oryza sativa; Lolium arundinaceum; Lotus japonicus; Cenchrus ciliaris; Populus tremula x Populus tremuloides; Nasturtium officinale; Picea glauca; Populus tremuloides; Populus trichocarpa; Prunus persica; Medicago truncatula; Nicotiana benthamiana; Hordeum vulgare; Ipomoea nil; Prunus armeniaca; Peridictyon sanctum; Oryza barthii; Hordeum vulgare subsp. vulgare; Oenocarpus bataua; Picea sitchensis; Brassica napus; Lupinus luteus; Phaseolus coccineus; Manihot esculenta; Ricinus communis; Oryza brachyantha; Cenchrus americanus; Phleum pratense; Panicum virgatum; Nuphar advena

Type:

Non-coding RNA profiling by array

Platform:

GPL17805

4 Samples

Download data: TXT