GEO DataSets

(Submitter supplied) Brucella spp. are gram-negative, facultative intracellular pathogens that cause brucellosis in humans and animals. Iron is an essential element required for all organisms. Iron response regulator (Irr) is a crucial transcriptional regulator and can affect the growth and iron uptake of Brucella. The growth rate of Brucella melitensis M5-90 irr mutant was significantly lower than that of B. melitensis M5-90 under normal or iron-sufficient conditions, however, the growth rate of the B. more...

Organism:

Brucella melitensis M5-90

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32729

6 Samples

Download data: XLSX

(Submitter supplied) Purpose: To uncover the related mechanisms underlie virulence attenuation of RM57 vaccine candidate. Methods: Three Brucella melitensis M1981 strains and three Brucella melitensis RM57 strains were grown in TSB at 37℃ until the log phase was reached, total RNA was isolated using the TRIzol according to the manufacturer’s instructions.The sequencing library of each RNA sample was prepared by using NEB Next Ultra Directional RNA Library Prep Kit for Illumina as recommended by the manufacturer.The HiSeq 4000 platform was used to perform the transcriptome sequencing. more...

Organism:

Brucella melitensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26681

6 Samples

Download data: TXT

(Submitter supplied) In a time course study, we characterized global gene expression profile of B. melitensis infection in bovine Peyer patches in the first 4 h p.i. Statistical analysis of microarray results reveal that 2,356 B. melitensis genes were detected as differentially expressed (z-score p < 0.025) compared with control between 15 min and 4 h post-infection. A group of 1,740 genes that were differentially expressed in at least 4 out of 5 time points was considered the core set of genes that reflect the major changes in B. more...

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL7612

44 Samples

Download data: GPR, TXT

(Submitter supplied) We sequenced the mRNA of Brucella melitensis 16M and otpR mutant under acid stress to identify genes that were regulated by regulator OtpR.

Organism:

Brucella melitensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17325

2 Samples

Download data: TXT, XLS

(Submitter supplied) We sequenced the transcriptomes of B. abortus 2308 wild type, rpoE1, and lovhK deletion strains to assess gene expression in response to oxidative stress. The data represent 2 independent biological replicates of each strain.

Organism:

Brucella melitensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18970

6 Samples

Download data: TAB

(Submitter supplied) In the intracellular pathogen Brucella spp., the activation of the stringent response, a global regulatory network providing rapid adaptation to a variety of growth-affecting stress conditions such as nutrient deficiency, is essential for replication in the host. A single, bi-functional enzyme Rsh catalyzes synthesis and hydrolysis of the alarmone (p)ppGpp, responsible for differential gene expression under stringent conditions. more...

Organism:

Brucella suis 1330; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL7612

4 Samples

Download data: TXT

(Submitter supplied) A whole genome expression comparision between wild type B. melitensis 16M and ΔBM-LOV-HK (ΔBMEII0679) B. melitensis strains grown in broth culture to determine genes directly and indirectly regulated by BM-LOV-HK.

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL16337

6 Samples

Download data: CALLS, PAIR

(Submitter supplied) Hfq is a bacterial Sm-like protein which serves as a post-transcriptional regulator of global gene expression. It has been shown to be involved in a wide range of cellular processes and pathways. To characterize the regulation roles of Hfq in Brucella melitensis, the in vitro hfq induction conditions simulating intracellular environments were firstly defined, and then, the transcriptome of the hfq mutant was compared to that of the wild type strain. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL11117

4 Samples

Download data: GPR

(Submitter supplied) The four-carbon sugar erythritol is an important component of B. melitensis pathogenesis. To determine the transcriptional response to erythritol, B. melitensis strain 16M was grown in the presence of either glucose or erythritol as a sole carbon source.

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL15533

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) Investigation of whole genome gene expression level changes in a Brucella melitensis delta prlr mutant compared to the wild type strain. The mutants analyzed in this study are further described in A. Mirabella, R-M Yanez, R.M. Delrue, S. Uzureau, M.S. Zygmunt, A. Cloeckaert, X. De Bolle, J.J. Letesson (2012). The two component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength. more...

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL5730

6 Samples

Download data: PAIR

(Submitter supplied) Brucella spp. is an intracellular pathogen in vivo. The intracellular B. melitensis transcriptome was determined by initially enriched and then amplified B. melitensis RNA from total RNA of B. melitensis-infected HeLa cells. Analysis of microarray results identified 161 and 115 genes differentially expressed at 4 and 12 h p.i., respectively. Most of the genes (78%) differentially expressed were down-regulated at the earliest time point, but up-regulated (75%) at 12 h p.i. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL8156

18 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brucella canis; Brucella melitensis bv. 1 str. 16M; Brucella melitensis; Brucella ovis

Type:

Expression profiling by array

Platforms:

GPL15024 GPL15023

16 Samples

Download data: CALLS, PAIR

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL15023

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) Quorum sensing system (QS) is a global regulator that regulates gene expression in response to cell density and other environmental factors. Two QS genes have been identified in Brucella, one of which (vjbR) is involved in Brucella intracellular survival and virulence. But the regulation mechanism of Brucella intracellular survival by the vjbR remains unknown.To characterize the regulation roles of vjbR, the in vitro vjbR induction conditions simulating intracellular environments were firstly defined, and then, transcriptome of the vjbR mutant is compared to that of the wild type strain. more...

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL11117

4 Samples

Download data: GPR

(Submitter supplied) The transcriptional regulator MucR was identified as highly attenuated in a mutagenesis screen conducted by this laboratory. MucR has been described as a key regulator of exopolysaccharide synthesis in closely related Rhizobials soil symbionts. The goal of this study was to apply custom Brucella melitensis oligonucleotide microarrays to identify the regulatory targets of MucR. These studies revealed that MucR's regulation of exopolysaccharide synthesis is also conserved in Brucella melitensis and is also a key regulator of iron sequestering/storage and denitrification. more...

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL7612

6 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL7612

30 Samples

Download data: GPR

(Submitter supplied) Quorum sensing (QS) is a communication system in bacteria that regulates gene expression in response to a small diffusible signal indicative of the bacterial population present. QS has been shown to regulate virulence genes, biofilm formation, cell division and secretion systems in a diverse set of bacteria. Brucella melitensis produces a QS signaling molecule, N-dodecanoyl homoserine lactone (C12-HSL), that interacts with a LuxR transcriptional regulator, VjbR. more...

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL7612

12 Samples

Download data: GPR

(Submitter supplied) Quorum sensing (QS) is a communication system in bacteria that regulates gene expression in response to a small diffusible signal indicative of the bacterial population present. QS has been shown to regulate virulence genes, biofilm formation, cell division and secretion systems in a diverse set of bacteria. Brucella melitensis produces a QS signaling molecule, N-dodecanoyl homoserine lactone (C12-HSL), that interacts with a LuxR transcriptional regulator, VjbR. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL7612

18 Samples

Download data: GPR

(Submitter supplied) Many pathogenic bacteria use a regulatory process termed Quorum Sensing (QS) to produce and detect small diffusible molecules to synchronize gene expression within a population. In Gram-negative bacteria, the detection and response to these molecules depend on transcriptional regulators belonging to the LuxR family. Such a system have been discovered in the intracellular pathogen Brucella melitensis, a Gram-negative bacteria responsible for brucellosis, a word-wide zoonosis remaining a serious public health concern in endemic countries. more...

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL5730

3 Samples

Download data: FTR

(Submitter supplied) In this study we report that B. melitensis at the late logarithmic phase of growth are more invasive for HeLa cells than at mid logarithmic or stationary growth phases. Microarray analysis of B. melitensis gene expression identified 414 up- and 40 down-regulated genes in late-log growth phase compared to the stationary growth phase. The vast majority of the up-regulated genes in late-log cultures were those associated with DNA replication, transcription and translation, intermediate metabolism, energy production and conversion, membrane transport and cell envelope, biogenesis and outer membrane, while the down-regulated genes were distributed among several functional categories. more...

Organism:

Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL6748

8 Samples

Download data: GPR