GEO DataSets

(Submitter supplied) Purpose: To analyze the toxicity effect of sheepshead minnow larvae exposed to PET microfibers through RNA seq. The expression of specific and sensitive genes on PET microfibers was confirmed and verified through qRT-PCR. Method: RNA was isolated from a sample exposed to PET microfibers (concentration of 10, 100 mg/L) for 10 days using a 72 hpf sheepshead minnow larvae. mRNA was produced with a TruSeq Standed mRNA library kit, and sequencing using a NovaSeq6000 equipment. more...

Organism:

Cyprinodon variegatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29741

4 Samples

Download data: FA, TXT

(Submitter supplied) To explore the methylation altering effects of oil on developing fish, we exposed larval Cyprinodon variegatus to oil for 48 h and used immunoprecipitation coupled with high-throughput sequencing (MeDIP seq) to evaluate genome-wide methylation changes.

Organism:

Cyprinodon variegatus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL29741

15 Samples

Download data: CSV

(Submitter supplied) The objective of this project was to expose larval Cyprinodon variegatus to oil/hypoxia stressors for 48 hrs, then allow 48 hours of recovery and depuration.

Organism:

Cyprinodon variegatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29741

30 Samples

Download data: CSV

(Submitter supplied) The objective of this project was to investigate the impacts of oil exposure in combination with environmental stressors on gene expression in developing C. variegatus.

Organism:

Cyprinodon variegatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25909

36 Samples

Download data: CSV

(Submitter supplied) To investigate molecular effects in fish following exposure to environmentally relevant concentrations of dispersants and DWH oil, we exposed adult sheepshead minnows (Cyprinodon variegatus) to Corexit 9500™ and two concentrations of high-energy water-accommodated fraction (HEWAF) or chemically enhanced water-accommodated fraction (CEWAF) for 7 and 14 days. Resulting changes in hepatic gene expression were measured using Agilent 8x15K microarrays. more...

Organism:

Cyprinodon variegatus

Type:

Expression profiling by array

Platform:

GPL23389

38 Samples

Download data: TXT

(Submitter supplied) Sheepshead minnow (Cyprinodon variegatus) Arrays of this design have barcodes that begin with 16027375 or 2527375. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. The ID column represents the Agilent Feature Extraction feature number. more...

Organism:

Cyprinodon variegatus

1 Series

38 Samples

Download data: TXT