GEO DataSets

(Submitter supplied) In the context of the Micro-Ecological Life Support System Alternative (MELiSSA) project, the quorum sensing system (QS) of R. rubrum S1H has been shown to rely on acyl homoserine lactones (AHLs) as communication signals. In addition, previous studies in our lab have suggested that pigment content and photosynthetic membrane production could be under the control of QS. In the present study, the transcriptomic and proteomic profiles of a QS-deficient mutant (R. more...

Organism:

Rhodospirillum rubrum ATCC 11170; Rhodospirillum rubrum

Type:

Expression profiling by array

Platform:

GPL7256

4 Samples

Download data: GPR

(Submitter supplied) In the present study, the susceptibility of the purple pigmented photosynthetic alphaproteobacterium Rhodospirillum rubrum S1H to gamma irradiation was investigated and its molecular response was characterised by means of gene expression analysis. R. rubrum S1H appears to be about 4 times more sensitive than the model strain Escherichia coli MG1655 to cobalt-60 gamma irradiation. Whole genome response of R. more...

Organism:

Rhodospirillum rubrum ATCC 11170; Rhodospirillum rubrum

Type:

Expression profiling by array

Platform:

GPL7256

9 Samples

Download data: GPR

(Submitter supplied) The present study explores the potential of compound-specific gene-upregulation profiles in the ubiquitous purple nonsulfur bacterium Rhodospirillum rubrum S1H as biomarkers for exposure to surface water contaminants, i.e. high production-volume pharmaceuticals. Even though the pharmaceuticals [i.e., acetylsalicylic acid (ASA), diclofenac (DCF), and 17α-ethinylestradiol (EE2)] did not affect the bacterial growth kinetics at environmentally-relevant concentrations (86nM), whole-genome microarray analyses revealed the upregulation of 128, 49, and 47 genes upon exposure to DCF, ASA, and EE2, respectively. more...

Organism:

Rhodospirillum rubrum; Rhodospirillum rubrum ATCC 11170

Type:

Expression profiling by array

Platform:

GPL7256

9 Samples

Download data: GPR

(Submitter supplied) In the frame of the European MELiSSA project, which aims to develop a closed biological life support system for forthcoming long term space exploration missions, the study of the alpha-proteobacterium Rhodospirillum rubrum S1H cultivated in space related environmental conditions has started. In the present work, the bacterium was grown using two different microgravity simulators, namely the Rotating Wall Vessel (RWV) and the Random Positioning Machine (RPM) and its response was evaluated at both the transcriptomic and proteomic levels using respectively a dedicated whole-genome microarray and high-througput proteomics. more...

Organism:

Rhodospirillum rubrum; Rhodospirillum rubrum ATCC 11170

Type:

Expression profiling by array

Platform:

GPL7256

2 Samples

Download data: GPR

(Submitter supplied) Aiming at the development of a micropollutant biosensor in the frame of the Micro-Ecological Life Support System Alternative (MELiSSA), a pilot study was initiated to identify triclosan (TCS)-responsive biomarker genes in the MELiSSA carbon-mineralizing microorganism, Rhodospirillum rubrum S1H. TCS is a biocide, commonly found in human excrements and is considered an emerging pollutant in wastewater and the environment. more...

Organism:

Rhodospirillum rubrum; Rhodospirillum rubrum ATCC 11170

Type:

Expression profiling by array

Platform:

GPL7256

2 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Rhodospirillum rubrum ATCC 11170; Rhodospirillum rubrum

Type:

Expression profiling by array

Platform:

GPL7256

6 Samples

Download data: GPR

(Submitter supplied) R. rubrum S1H inoculated on solid minimal media was sent to the ISS in September 2006 (BASE-A experiment). After 10 days flight, R. rubrum cultures returned back to Earth. These cultures were then subjected to both transcriptomic and proteomic analysis and compared with the corresponding ground control. Whole-genome oligonucleotide microarray and high throughput proteomics, which offer the possibility to survey respectively the global transcriptional and translational response of an organism, were used to test the effect of space flight. more...

Organism:

Rhodospirillum rubrum; Rhodospirillum rubrum ATCC 11170

Type:

Expression profiling by array

Platform:

GPL7256

3 Samples

Download data: GPR

(Submitter supplied) R. rubrum S1H inoculated on solid agar rich media was sent to the ISS in October 2003 (MESSAGE-part 2 experiment). After 10 days flight, R. rubrum cultures returned back to Earth. These cultures were then subjected to both transcriptomic and proteomic analysis and compared with the corresponding ground control. Whole-genome oligonucleotide microarray and high throughput proteomics, which offer the possibility to survey respectively the global transcriptional and translational response of an organism, were used to test the effect of space flight. more...

Organism:

Rhodospirillum rubrum ATCC 11170; Rhodospirillum rubrum

Type:

Expression profiling by array

Platform:

GPL7256

3 Samples

Download data: GPR

(Submitter supplied) 1. Oligos are synthesized and arrayed in Greiner 96-deep well plates. Each well contains more than 10nmol of 60-mer oligo and are resuspended in water to 10µM. 2. Resuspended oligos in water to 10 µM are rearrayed into 384-well (10µl transfered from 96 to 384 well plates), Genetix polystyrene V-bottom plates (cat# X6004). 3. 10µl of 2x spotting buffer is added to each well of the 384 plate; these plates are considered as spotting master plates. more...

Organism:

Rhodospirillum rubrum ATCC 11170

8 Series

32 Samples

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