GEO DataSets

(Submitter supplied) We used comparative transcriptomics to explore cellular responses to growth on pyrite (FeS2) or aqueous iron (Fe(II)) and sulfur (cysteine or sulfide). Transcriptomic data from wild type M. barkeri identified subset of genes that was significantly upregulated during grown on FeS2 versus ferrous iron and cysteine or sulfide. Several of these genes, including a membrane-bound hydrolase, alpha-keto reductases, and flavin mononucleotide-dependent flavodoxin reductases were highly conserved among known FeS2-reducing methanogens and were located in a single gene cassette. more...

Organism:

Methanosarcina barkeri

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29851

12 Samples

Download data: TXT

(Submitter supplied) Hydrogenases are a critical component of H2-dependent energy-conservation pathways in Methanosarcina barkeri. To allow phenotypic analysis of the hydrogenases, we constructed mutants lacking the frhADGB, freAEGB, vhtGACD, vhxGAC and echABCDEF operons, individually and in all possible combinations. In addition to measuring the effect of each deletion on growth, methane production, and hydrogenase activity, the effect on gene expression was measured by RNA sequencing to detect potential transcriptional regulation by the hydrogenases.

Organism:

Methanosarcina barkeri str. Fusaro

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20638

27 Samples

Download data: XLSX

(Submitter supplied) The goal of this work was to elucidate the mechanism by which pyruvate is utilized as a substrate in a mutant strain of Methanosarcina barkeri Fusaro. In this study, using RNAseq we gained insight into how the mutant strain modulate its transcriptional profile in order to use pyruvate as a substrate. In addition, we obtained information on how methanogens respond to pyruvate at the transcriptional level.

Organism:

Methanosarcina barkeri str. Fusaro

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20638

54 Samples

Download data: XLSX