GEO DataSets

(Submitter supplied) Bio-augmentation could be a promising strategy to improve processes for treatment and resource recovery from wastewater. In this study, the Gram-positive bacterium Bacillus subtilis was co-cultured with the microbial communities present in wastewater samples with high concentrations of nitrate or ammonium. Glucose supplementation (1%) was used to boost biomass growth in all wastewater samples. In anaerobic conditions, the indigenous microbial community bio-augmented with B. more...

Organism:

Clostridium butyricum; Clostridium beijerinckii

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23579 GPL28541

32 Samples

Download data: CSV

(Submitter supplied) In this study, we studied the fibrolytic potential of the rumen microbiota in the rumen of 6 lambs separated from their dams from 12h of age and artificially fed with milk replacer (MR) and starter feed from d8, in absence (3 lambs) or presence (3 lambs) of a combination of the live yeast Saccharomyces cerevisiae CNCM I-1077 and selected yeast metabolites. The fibrolytic potential of the rumen microbiota of the lambs at 56 days of age was analyzed with a DNA microarray (FibroChip) targeting genes coding for 8 glycoside hydrolase (GH) families.

Organism:

Selenomonas ruminantium; Lactococcus lactis; Clostridioides difficile; Ruminiclostridium cellulolyticum; Limosilactobacillus fermentum; Cellulomonas flavigena; Neocallimastix patriciarum; Bifidobacterium animalis; Agathobacter rectalis; Enterobacter sp.; Orpinomyces joyonii; Piromyces rhizinflatus; Piromyces sp. 'equi'; Pseudobutyrivibrio xylanivorans; Bifidobacterium longum; Bacteroides thetaiotaomicron; Fibrobacter succinogenes; Clostridium cellulovorans; Bifidobacterium adolescentis; Cellulomonas fimi; Neocallimastix frontalis; [Eubacterium] cellulosolvens; Lachnospira eligens; Ruminococcus sp.; Piromyces sp.; Roseburia intestinalis; Roseburia hominis; Bacteroides fragilis; Xylanibacter ruminicola; Ruminococcus albus; Enterococcus faecium; Clostridium acetobutylicum; Acetivibrio thermocellus; Clostridium beijerinckii; Levilactobacillus brevis; Microbiota; Bacteroides ovatus; Fibrobacter intestinalis; Bacteroides sp.; Epidinium caudatum; Polyplastron multivesiculatum; Butyrivibrio hungatei; Epidinium ecaudatum; Bacteroides xylanisolvens; Cellulosilyticum ruminicola; Ruminococcus champanellensis; Orpinomyces sp.; Butyrivibrio fibrisolvens; Ruminococcus flavefaciens; Piromyces communis; Pseudobacteroides cellulosolvens; Eudiplodinium maggii; Segatella bryantii; Acetivibrio clariflavus; uncultured Neocallimastigales

Type:

Genome variation profiling by array

Platform:

GPL25777

6 Samples

Download data: TXT

(Submitter supplied) We investigated an improved method that combines competitive PCR and microarray techniques. This approach allowed us to quantify specific bacterial groups mounted on DNA chips with accuracy close to that of real-time PCR, despite a measurement at the end point of PCR, and also to estimate the bacterial DNA content in sample DNA.

Organism:

Campylobacter rectus; Helicobacter pylori; Pseudomonas aeruginosa; Aggregatibacter actinomycetemcomitans; Phocaeicola vulgatus; Capnocytophaga gingivalis; Deinococcus radiodurans; Streptococcus mutans; Streptococcus intermedius; Cutibacterium acnes; Neisseria meningitidis; Porphyromonas gingivalis; Fusobacterium nucleatum; Clostridium beijerinckii; Lactobacillus gasseri; Tannerella forsythia; Treponema denticola; Cereibacter sphaeroides; Streptococcus gordonii; Streptococcus agalactiae; Enterococcus faecalis; Bifidobacterium adolescentis; Homo sapiens; Prevotella intermedia; Prevotella nigrescens; Bacteria; Acinetobacter baumannii; Escherichia coli; Staphylococcus aureus; Staphylococcus epidermidis; Bacillus cereus; Schaalia odontolytica; Fusobacterium nucleatum subsp. nucleatum

Type:

Other

Platform:

GPL25612

178 Samples

Download data: CSV

(Submitter supplied) Purpose:C. beijerinckii DSM 6423 is the most cited natural IpOH producer. Improving the natural production of this strain through a targeted approach required a full sequencing and characterization of its genome, together with transcriptomic analyses of its own regulations.The goals of this study are then to evaluate the transcriptional profile (RNA-Seq) of C. beijerinckii DSM6423, a natural isopropanol producer, during a fermentation of glucose in controlled bioreactors. more...

Organism:

Clostridium beijerinckii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23579

15 Samples

Download data: CSV

(Submitter supplied) Ruminants are the most efficient herbivorous animals to transform plant biomass into edible products, principally thanks to the rumen microbiota that produces a large array of enzymes responsible for the hydrolysis of plant cell wall polysaccharides. Several biotic and abiotic factors influence the efficiency of fiber degradation, which can ultimately impact the animal productivity and health. To provide more insight on mechanisms involved in the modulation of fibrolytic activity, a functional DNA microarray targeting genes coding for key enzymes involved in cellulose and hemicellulose degradation by rumen microbiota was designed. more...

Organism:

Butyrivibrio fibrisolvens; Selenomonas ruminantium; Lactococcus lactis; Clostridioides difficile; Ruminiclostridium cellulolyticum; Cellulomonas flavigena; Neocallimastix patriciarum; Bifidobacterium animalis; Agathobacter rectalis; Enterobacter sp.; Escherichia coli K-12; Pseudobutyrivibrio xylanivorans; Bifidobacterium longum; uncultured Neocallimastigales; Bacteroides thetaiotaomicron; Fibrobacter succinogenes; Xylanibacter ruminicola; Clostridium acetobutylicum; Clostridium cellulovorans; Bifidobacterium adolescentis; Cellulomonas fimi; Neocallimastix frontalis; Bacteroides ovatus; [Eubacterium] cellulosolvens; Bacteroides sp.; Lachnospira eligens; Ruminococcus sp.; Piromyces sp.; Roseburia intestinalis; Butyrivibrio hungatei; Roseburia hominis; bovine gut metagenome; Bacteroides xylanisolvens XB1A; Orpinomyces sp.; Bacteroides fragilis; Ruminococcus albus; Enterococcus faecium; Acetivibrio thermocellus; Clostridium beijerinckii; Levilactobacillus brevis; Fibrobacter intestinalis; Epidinium caudatum; Polyplastron multivesiculatum; Orpinomyces joyonii; Piromyces rhizinflatus; Piromyces sp. 'equi'; Epidinium ecaudatum; Bacteroides xylanisolvens; Cellulosilyticum ruminicola; Ruminococcus champanellensis; Ruminococcus flavefaciens; Limosilactobacillus fermentum; Piromyces communis; Pseudobacteroides cellulosolvens; Eudiplodinium maggii; Fibrobacter succinogenes subsp. succinogenes S85; Segatella bryantii; Acetivibrio clariflavus

Type:

Expression profiling by array; Other

Platform:

GPL24327

14 Samples

Download data: TXT

(Submitter supplied) Solventogenic Clostridium species ferment carbohydrates to acetone, butanol and ethanol which are well-known next-generation biofuels. However, repeated subculture of or continuous fermentation by Clostridium often decreases and eventually terminates the solvent production and spore formation, which is a process called strain degeneration. Supplementation of CaCO3 to fermentation medium could partially recover metabolism of degenerated strain by more than 50% increase of cell growth and solvent production. more...

Organism:

Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL19469

6 Samples

Download data: TXT

(Submitter supplied) The transcriptome analysis by microarray was applied on wild-type and degenerated strains of C. beijerinckii NCIMB8052 (WT-8052 and DG-8052, respectively) to elucidate its mechanism of degeneration during solvent fermentation. The comparison of gene expression pattern in relation to ABE production is expected to provide insights toward metabolically engineering of C. beijerinckii NCIMB8052 with prevention of degeneration and eventually enhancement of ABE production.

Organism:

Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL19469

6 Samples

Download data: TXT

(Submitter supplied) The impacts of ferulic acid, a phenolic compound commonly found in lignin hydrolysates, on the growth, solvent production and transcriptional responses of C. beijerinckii NCIMB 8052 were determined. Addition of ferulic acid to growing cultures resulted in a decrease in the growth and solvent production by 30 and 25%, respectively, when compared to the control cultures. To better understand the toxicity of this compound, microarray analyses were performed using samples taken from these cultures at three different growth states. more...

Organism:

Clostridium beijerinckii; Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL19923

16 Samples

Download data: TXT

(Submitter supplied) Furfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulosic biomass to monomeric sugars, but the molecular response of Clostridium beijerinckii NCIMB 8052 to this compound is unknown. To discern the effect of furfural on C. beijerinckii and to gain insights into the molecular mechanisms of action and detoxification, we studied the physiological changes of furfural-stressed cultures during acetone-butanol-ethanol (ABE) fermentation, and profiled differentially expressed genes by genome-wide transcriptional analysis. more...

Organism:

Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL16336

4 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Clostridium beijerinckii; Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL6990

21 Samples

Download data: GPR

(Submitter supplied) The fermentation culture of Clostridium beijerinckii mutant BA101 was monitored from exponential growth to stationary phase. During this period the culture underwent a shift from acidogenesis to solventogenesis. Acetone and butanol production was initiated with the onset of the solventogenic phase. Using DNA microarray changes in gene expression were examined during the transitional period.

Organism:

Clostridium beijerinckii NCIMB 8052; Clostridium beijerinckii

Type:

Expression profiling by array

Platform:

GPL6990

11 Samples

Download data: GPR

(Submitter supplied) The Clostridium beijerinckii NCIMB 8052 wild-type culture was monitored from exponential growth to stationary phase. During this period the culture underwent a shift from acidogenesis to solventogenesis. Acetone and butanol production was initiated with the onset of the solventogenic phase. Using DNA microarray changes in gene expression were examined during the transitional period.

Organism:

Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL6990

10 Samples

Download data: GPR

(Submitter supplied) see manufacturer's web site at https://www.genomics.agilent.com/en/Custom-Gene-Expression-Microarrays/Custom-Gene-Expression-Microarrays/?cid=AG-PT-132&tabId=AG-PR-1038

Organism:

Bacteroides fragilis; Bacteroides thetaiotaomicron; Butyrivibrio fibrisolvens; Fibrobacter succinogenes; Xylanibacter ruminicola; Selenomonas ruminantium; Ruminococcus albus; Ruminococcus flavefaciens; Enterococcus faecium; Lactococcus lactis; Clostridium acetobutylicum; Clostridium cellulovorans; Clostridioides difficile; Acetivibrio thermocellus; Clostridium beijerinckii; Ruminiclostridium cellulolyticum; Levilactobacillus brevis; Limosilactobacillus fermentum; Bifidobacterium adolescentis; Cellulomonas fimi; Cellulomonas flavigena; Neocallimastix frontalis; Neocallimastix patriciarum; Piromyces communis; Bifidobacterium animalis; Bacteroides ovatus; Fibrobacter intestinalis; [Eubacterium] cellulosolvens; Bacteroides sp.; Pseudobacteroides cellulosolvens; Lachnospira eligens; Agathobacter rectalis; Ruminococcus sp.; Enterobacter sp.; Piromyces sp.; Epidinium caudatum; Eudiplodinium maggii; Polyplastron multivesiculatum; Orpinomyces joyonii; Piromyces rhizinflatus; Segatella bryantii; Piromyces sp. 'equi'; Roseburia intestinalis; Pseudobutyrivibrio xylanivorans; Butyrivibrio hungatei; Bifidobacterium longum; Acetivibrio clariflavus; Epidinium ecaudatum; Roseburia hominis; uncultured Neocallimastigales; Bacteroides xylanisolvens; Cellulosilyticum ruminicola; Ruminococcus champanellensis; Orpinomyces sp.

1 Series

6 Samples

Download data

(Submitter supplied) see manufacturer's web site at https://www.genomics.agilent.com/en/Custom-Gene-Expression-Microarrays/Custom-Gene-Expression-Microarrays/?cid=AG-PT-132&tabId=AG-PR-1038

Organism:

Bacteroides fragilis; Bacteroides thetaiotaomicron; Butyrivibrio fibrisolvens; Fibrobacter succinogenes; Xylanibacter ruminicola; Selenomonas ruminantium; Ruminococcus albus; Ruminococcus flavefaciens; Enterococcus faecium; Lactococcus lactis; Clostridium acetobutylicum; Clostridium cellulovorans; Clostridioides difficile; Acetivibrio thermocellus; Clostridium beijerinckii; Ruminiclostridium cellulolyticum; Levilactobacillus brevis; Limosilactobacillus fermentum; Bifidobacterium adolescentis; Cellulomonas fimi; Cellulomonas flavigena; Neocallimastix frontalis; Neocallimastix patriciarum; Piromyces communis; Bifidobacterium animalis; Bacteroides ovatus; Fibrobacter intestinalis; [Eubacterium] cellulosolvens; Bacteroides sp.; Pseudobacteroides cellulosolvens; Lachnospira eligens; Agathobacter rectalis; Ruminococcus sp.; Enterobacter sp.; Piromyces sp.; Epidinium caudatum; Eudiplodinium maggii; Polyplastron multivesiculatum; Orpinomyces joyonii; Piromyces rhizinflatus; Segatella bryantii; Piromyces sp. 'equi'; Roseburia intestinalis; Pseudobutyrivibrio xylanivorans; Butyrivibrio hungatei; Bifidobacterium longum; Acetivibrio clariflavus; Epidinium ecaudatum; Roseburia hominis; uncultured Neocallimastigales; Bacteroides xylanisolvens; Cellulosilyticum ruminicola; Ruminococcus champanellensis; Orpinomyces sp.

1 Series

14 Samples

Download data

(Submitter supplied) see manufacturer's web site at http://www.agilent.com/

Organism:

Clostridium beijerinckii NCIMB 8052

2 Series

12 Samples

Download data

(Submitter supplied) Arrays of this design have barcodes that begin with 16038215 or 2538215. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. The ID column represents the Agilent Feature Extraction feature number. more...

Organism:

Clostridium beijerinckii

1 Series

16 Samples

Download data: TXT

(Submitter supplied) Each array has 30,720 potential addresses for probes, of which 25,015 spots have 45-47mer probes for Clostridium beijerinckii NCIMB 8052 (Cb) genes, 4,985 features have no probe, 608 features have MYcroarray in-house QC probes and 112 features have positive control probes. Each gene is appraised by one unique probe sequence. There are 5 identical replicates of each Cb probe sequence such that a total of 5,003 Cb genes are appraised by each array. more...

Organism:

Clostridium beijerinckii NCIMB 8052

1 Series

4 Samples

Download data

(Submitter supplied) The draft genome sequence assembly of Clostridium beijerinckii (whole genome shotgun [WGS] project accession AALO00000000) was used as a source of the genes used in this study. Note, this WGS project has been superceded by the complete genome in (non-WGS) GenBank record CP000721. The Platform data table reflects a condensed representation of the array's replicate features. The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record. more...

Organism:

Clostridium beijerinckii NCIMB 8052

3 Series

21 Samples

Download data: TXT