GEO DataSets

(Submitter supplied) Group B Streptococcus (GBS) is a pathobiont responsible for invasive infections in neonates and in the elderly. The transition from a commensal to an invasive pathogen relies on the timely regulation of a set of virulence factors. In this study, we characterized the role of the SaeRS two-component system in GBS pathogenesis. Loss-of-function mutations in the SaeR response regulator decrease virulence in mouse models of invasive infection . more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

12 Samples

Download data: TXT

(Submitter supplied) Streptococcus agalactiae is among the few pathogens that have not developed resistance to ß-lactam antibiotics despite decades of clinical use. The molecular basis of this long-lasting susceptibility has not been investigated, and it is uncertain whether specific mechanisms constraint the emergence of resistance. In this study, we first report a conserved role of the signaling nucleotide cyclic-di-AMP in the sensitivity of S. more...

Organism:

Streptococcus agalactiae; Streptococcus agalactiae NEM316

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29157 GPL34325

18 Samples

Download data: TXT

(Submitter supplied) Each bacterial species has specific regulatory systems to control physiology, adaptation, and host interactions. One challenge posed by this diversity is to define the evolving gene regulatory networks. This study aims to characterise two-component systems (TCS) in Streptococcus gagalactiae, the main cause of neonatal meningitis. Here we demonstrate signal-independent activation of signalling pathways by systematically targeting the conserved mechanism of phosphatase activity of the histidine kinases of the two main TCS families. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

48 Samples

Download data: XLS

(Submitter supplied) Group B Streptococcus (GBS) strain CNCTC 10/84 was modified to have specific mutations to the cas9 gene: allelic exchange replacement with a chloramphenicol resistance marker (Cas9 knockout), D10A and H845A (catalytically inactive dCas9), or R1339A and R1441A (sCas9 unable to scan for protospacer adjacent motifs). Wild type (WT) and mutant strains were grown in biological triplicate samples and used for RNA purification at two growth timepoints: OD600=0.6 and OD600=1.2. more...

Organism:

Streptococcus agalactiae CNCTC 10/84

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33193

36 Samples

Download data: TXT

(Submitter supplied) Diabetic wound infections have poor healing outcomes due to the presence of numerous pathogens in addition to an impaired immune response. Group B Streptococcus (GBS) is one of the most commonly isolated bacteria from diabetic wound infections, but virulence mechanisms GBS uses during these infections have not been investigated. Here, we developed a new murine model of GBS diabetic wound infection to determine how GBS establishes infection and persists in the wound environment. more...

Organism:

Streptococcus agalactiae; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32194 GPL32195 GPL13112

15 Samples

Download data: XLSX

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in CopY (∆copY strain GU2857), grown in Todd-Hewitt broth (THB) medium supplemented with 0.25 mM Zn and sequenced using Illumina NextSeq500.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Sa strain 874391 and human U937 monocytes at 6h post-infection

Organism:

Homo sapiens; Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18573 GPL29157 GPL29372

12 Samples

Download data: XLSX

(Submitter supplied) The goal of this analysis was to identify genes that promote survival of S. agalactiae within macrophages.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23396

3 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Streptococcus agalactiae

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL29158 GPL23396 GPL29157

40 Samples

Download data: BEDGRAPH, NARROWPEAK, TXT

(Submitter supplied) Streptococcus agalactiae, also known as Group B streptococcus, emerged in the 1960s as a leading cause of septicemia and meningitis in neonates. It is also an increasing cause of infections in adults with underlying diseases. To characterize transcription start sites (TSS) in the hypervirulent ST17 lineage (strain BM110) we used a differential RNA-seq strategy, based on selective Tobacco Acid Pyrophosphatase (TAP) treatment and adapter ligation, which differentiates primary transcripts and processed RNAs

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29158

6 Samples

Download data: XLSX

(Submitter supplied) To define the transcriptional response associated to a CovR inactivation we performed RNA-Seq in GBS strains BM110 and NEM316. We used mutants in which CovR is inactivated following a two base-pairs chromosomal substitution (AT->CC) resulting in the translation of a CovRD53A variant unable to be phosphorylated by the histidine kinase CovS. We also used a ∆covR mutant in strain BM110 in which the covR sequence is deleted from the chromosome.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23396

18 Samples

Download data: XLS

(Submitter supplied) We report the characterization of the major regulator of virulence gene expression (CovR) in Group B Streptococcus. The ChIP-seq experiments define the binding of CovR on the chromosome of the BM110 strain, a representative of the hypervirulent GBS lineage responsible of neonatal meningitis. Regulatory evolution of CovR signaling was investigated by comparing ChIP-seq done in parallel in a second GBS clinical isolate (NEM316) not belonging to the hypervirulent lineage.

Organism:

Streptococcus agalactiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL29157

16 Samples

Download data: BEDGRAPH, NARROWPEAK, TXT

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in SczA (∆sczA strain GU2791), grown in Todd-Hewitt broth (THB) medium supplemented with 0.5 mM Cu and sequenced using Illumina NextSeq500.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in SczA (∆sczA strain GU2791), grown in Todd-Hewitt broth (THB) medium supplemented with 0.25 mM Zn and sequenced using Illumina NextSeq500.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in SczA (∆sczA strain GU2791), grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in CovR (∆covR strain GU2400), grown in Todd-Hewitt broth (THB) medium, supplemented with 0.5 mM Cu and sequenced using Illumina NextSeq500

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in CovR (∆covR strain GU2400), grown in Todd-Hewitt broth (THB) medium, supplemented with 0.25 mM Zn and sequenced using Illumina NextSeq500

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in CovR (∆covR strain GU2400), grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in CopY (∆copY strain GU2857), grown in Todd-Hewitt broth (THB) medium supplemented with 0.5 mM Cu and sequenced using Illumina NextSeq500.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in CopY (∆copY strain GU2857), grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX