GEO DataSets

(Submitter supplied) To understand what molecular mechanisms determine the unique interspecies interactions between two predominant oral commensals Streptococcus sanguinis and Corynebacterium durum in the healthy oral cavity. We further investigated the regulatory connection between lipid metabolism and chain elongation based on the global gene expression profiling of the dual species cultures, as well as characterized S. more...

Organism:

Streptococcus sanguinis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33360

6 Samples

Download data: TXT

(Submitter supplied) To look for transcriptomic changes that resulted in membrane vesicle biogenesis, we performed RNA sequencing on S.sanguinis in different culture and community conditions We then examined three separate differential expression analyses, as well as correlated the phenotypic vesicle quantification with normalized count data across all samples.

Organism:

Streptococcus sanguinis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33159

32 Samples

Download data: TXT

(Submitter supplied) RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture, in coculture with Streptococcus gordonii DL1, Streptococcus sanguinis SK36 or Streptococcus oralis 34, and in a quadculture containing all four species. Individual cultures of commensal species Streptococcus gordonii DL1, Streptococcus sanguinis SK36 and Streptococcus oralis 34 were sequenced as well. more...

Organism:

Streptococcus gordonii; Streptococcus sobrinus; Lacticaseibacillus casei; Streptococcus mutans UA159; Streptococcus sanguinis; Streptococcus mutans; Streptococcus oralis; Corynebacterium matruchotii

Type:

Expression profiling by high throughput sequencing

11 related Platforms

33 Samples

Download data: CSV, XLSX

(Submitter supplied) Recently identified spontaneous mutants of major glucose-PTS (manLMNO) in stocks of Streptococcus sanguinis SK36 showed enhanced fitness in low-pH environment. Transcriptomic and metabolomic analyses of the manL mutant (SK36/manL) revealed redirection of pyruvate from production of lactate to acetate for extra energy extraction, resulting in excretion of greater amounts of pyruvate and H2O2, and increased expression of multiple alkali-generating activities. more...

Organism:

Streptococcus sanguinis SK36

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32504

6 Samples

Download data: XLSX

(Submitter supplied) The purpose of this study was to determine what genes were differentially expressed in a Streptococcus sanguinis mutant deleted for the SsaACB manganese transporter, which underwent growth arrest upon shifting the pH to 6.2 with HCl vs. its wild-type parent, SK36, which continued to grow normally. The WT and mutant strain, JFP169, were grown in a fermenter with minimal aeration in BHI broth. RNA-seq was used to compare gene expression from samples taken 20 minutes prior to addition of HCl to samples taken 10, 25, and 50 min after addition. more...

Organism:

Streptococcus sanguinis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25823

32 Samples

Download data: CSV

(Submitter supplied) The purpose of this study was to determine why manganese is required for the growth of Streptococcus sanguinis. The study employed a mutant derivative of S. sanguinis strain SK36 deleted for the genes encoding the manganese transporter SsaACB. The mutant strain, JFP169, was grown in a fermenter with constant aeration in BHI broth. RNA-seq was used to compare gene expression from samples taken 20 minutes prior to addition of 100 micromolar EDTA to samples taken 10, 25, and 50 min after addition. more...

Organism:

Streptococcus sanguinis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25823

16 Samples

Download data: TSV

(Submitter supplied) Gene expression profile of the Streptococcus sanguinis SSA_0351 deletion mutant, ssx_0351, compared to the wild-type strain SK36

Organism:

Streptococcus sanguinis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22689

8 Samples

Download data: TXT

(Submitter supplied) Gene expression profile of the Streptococcus sanguinis transcription regulator, brpL, deletion mutant compared to the wild-type strain SK36

Organism:

Streptococcus sanguinis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22689

16 Samples

Download data: TXT

(Submitter supplied) Gene expression profile of the Streptococcus sanguinis SK36 exposed to heat (43 C) for three time periods compared to the untreated strain SK36.

Organism:

Streptococcus sanguinis SK36

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23251

12 Samples

Download data: TXT

(Submitter supplied) Gene expression profile of the Streptococcus sanguinis SK36 exposed to sub-inhibitory dose (0.125 µg/ml) of Ampicillin for three time periods compared to the untreated strain SK36

Organism:

Streptococcus sanguinis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22689

12 Samples

Download data: TXT

(Submitter supplied) Gene expression profile of the Streptococcus sanguinis two component system regulator, ciaR, deletion mutant compared to the wild-type strain SK36

Organism:

Streptococcus sanguinis SK36

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23251

9 Samples

Download data: TXT

(Submitter supplied) Gene expression profile of the Streptococcus sanguinis TetR family transcriptional regulator, brpT, deletion mutant compared to the wild-type strain SK36

Organism:

Streptococcus sanguinis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22689

10 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of Streptococcus sanguinis nox mutant and its complemented strain compared to the wild-type SK36.

Organism:

Streptococcus sanguinis

Type:

Expression profiling by array

Platform:

GPL14967

6 Samples

Download data: MEV

(Submitter supplied) We found that many of the genes for which mutants could not be obtained using promoterless aphA-3 gene replacement were annotated as acquired via horizontal gene transfer or as encoding hypothetical proteins. We were curious about the explanation of this finding. The expression of these genes was therefore examined by microarray analysis. The microarray data showed many had undetectable expression. This led us to suspect that many of the unrecoverable mutants might have resulted from insufficient expression of the promoterless aphA-3 gene.

Organism:

Streptococcus sanguinis

Type:

Expression profiling by array

Platform:

GPL5902

3 Samples

Download data: MEV

(Submitter supplied) Transcriptional profiling of ssa_1972-null mutant of Streptococcus sanguinis compared with wild type. The ssa_1972 gene was inactivated in Streptococcus sanguinis SK36 and transcriptional profile was compared with wild type SK36. More information can be found at http://www.people.vcu.edu/~pingxu

Organism:

Streptococcus sanguinis; Streptococcus sanguinis SK36

Type:

Expression profiling by array

Platform:

GPL14967

3 Samples

Download data: MEV

(Submitter supplied) In a transcriptome based trail, we figured out that the deletion of luxS has a massive influence to cell growth and metabolism of Streptococcus sanguinis SK36. The biofilm defective luxS deletion mutant was comlemented by transgenic sahH from Pseudomonas aeruginosa. Thus 209 of 216 influenced genes of the luxS mutant compared to the isogenic wildype were restored in their expression (fold change of n ≥ 3; p ≤ 0.05), including genes involved in cell division processes, stress response and catabolite control. more...

Organism:

Streptococcus sanguinis SK36

Type:

Expression profiling by array

Platform:

GPL15399

12 Samples

Download data: CALLS, PAIR

(Submitter supplied) Investigation of whole genome gene expression levels of P. gingivalis W83, F. nucleatum DSMZ 25586, S. sanguinis SK36, A. actinomycetemcomitans HK1651, S. mutans UA159 in an 24 h old culture. Additionally, whole genome gene expression level changes of S. mutans UA159 biofilm cells after co-cultivation with S. mitis ATCC 11843 were compared to its single species biofilm growth after 24 h. Aim: Demonstration of the usefulness of a five-species gene expression array. more...

Organism:

Streptococcus mutans UA159; Porphyromonas gingivalis W83; Fusobacterium nucleatum; Aggregatibacter actinomycetemcomitans HK1651; Streptococcus mitis; Streptococcus sanguinis SK36

Type:

Expression profiling by array

Platforms:

GPL13444 GPL10873

12 Samples

Download data: CALLS, PAIR, TXT

(Submitter supplied) Transcriptional profiling of the spxA1-null mutant of Streptococcus sanguinis SK36 compared with wild type. The spxA1 gene was inactivated in Streptococcus sanguinis SK36, and the mutant demonstrated opaque colony morphology, reduced hydrogen peroxide (H2O2) production, and reduced antagonistic activity against Streptococcus S. mutans UA159 both on plates and in liquid media. The mutant also showed decreased tolerance to high temperature, and acidic and oxidative stresses. more...

Organism:

Streptococcus sanguinis

Type:

Expression profiling by array

Platform:

GPL14967

3 Samples

Download data: MEV

(Submitter supplied) Streptococcus sanguinis is a major component of the oral flora and an important cause of infective endocarditis. The genome sequence of S. sanguinis strain SK36 was recently determined. A number of foreign genes acquired by natural transformation were detected, as well as orthologs of competence genes previously identified in other species. However, significant differences in the S. sanguinis competence system relative to that of other streptococci were noted. more...

Organism:

Streptococcus sanguinis

Type:

Expression profiling by array

Platform:

GPL5902

29 Samples

Download data: GPR