(Submitter supplied) Current next-generation RNA sequencing methods do not provide accurate quantification of small RNAs within a sample due to sequence-dependent biases in capture, ligation, and amplification during library preparation. We present a method – AQRNA-seq – that minimizes biases and provides a direct, linear correlation between sequencing read count and copy number for small RNAs in a sample. The library preparation and data processing steps were optimized and validated using a 963-member microRNA reference library, oligonucleotide standards of varying lengths, and northern blots.
more...- Organism:
- Homo sapiens
- Type:
- Non-coding RNA profiling by high throughput sequencing
- Platform:
- GPL18573
- 15 Samples
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