GEO DataSet Links for PMC (Select 1867595) - GEO DataSets

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Items: 2

Select item 2000032541.

Variability in Microarray Labeling Methods

(Submitter supplied) Considerable variation in gene expression data from different DNA microarray platforms has been demonstrated. However, no characterization of the source of variation arising from labeling protocols has been performed. To analyze the variation associated with T7-based RNA amplification/labeling methods, aliquots of the Stratagene Human Universal Reference RNA were labeled using 3 eukaryotic target preparation methods and hybridized to a single array type (Affymetrix U95Av2). more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS1954
Platform:: GPL8300
20 Samples

Download data: CEL, EXP

Series

Accession:: GSE3254

ID:: 200003254

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 19542.

Various T7 RNA polymerase-based RNA amplification/labeling methods

Comparison of expression profiles generated using various T7 RNA polymerase-based in vitro transcription (IVT) RNA labeling methods. The length of the IVT reactions ranged from 4 to 16 hours. Results provide evidence for amplification method-dependent biases in gene expression data.