GEO DataSets

Analysis of undifferentiated H1 and H9 embryonic stem cell (ESC) lines and their differentiated embryoid bodies (EB). Result provide insight into those genes consistently altered during ESC differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell line, 2 protocol sets

Platform:

GPL570

Series:

GSE54186

8 Samples

Download data: CEL

(Submitter supplied) Comparison of gene expression signatures in undifferentiated hESCs against differentiated embryoid bodies to identify key signatures defining self-renewal of hESCs.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5408

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) To realize the full potential of human embryonic stem cells (hESC), it is important to develop culture conditions that maintain hESC in a pluripotent, undifferentiated state. A low O2 atmosphere (~4% O2), for example, prevents spontaneous differentiation and supports self-renewal of hESC. To identify genes whose expression is sensitive to O2 conditions, microarray analysis was performed on RNA from hESC that had been maintained under either 4% or 20% O2. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Human embryonic stem cells (hESCs) replicate by the process of self-renewal, whilst maintaining their pluripotency. Understanding the pathways involved in the regulation of this self-renewal process will assist in developing fully-defined conditions for the proliferation of hESCS required for therapeutic applications. We previously demonstrated a role for Sphingosine-1-phosphate (S1P) in the survival and proliferation of hESCs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2832

Platform:

GPL570

8 Samples

Download data: CEL

Analysis of embryonic stem cells (ESCs) after treatment with sphingosine-1-phosphate (S1P) which plays a role in regulation of fate in many cell types. ESCs grown on mouse embryo fibroblast feeder cells and under feeder-free conditions. Results provide insight into the role of S1P in ESC renewal.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 growth protocol sets

Platform:

GPL570

Series:

GSE7896

8 Samples

Download data: CEL

(Submitter supplied) Whole genome expression analysis reveals that single blastomeres from day-3 human embryos and blastocysts show unique gene signature, thus hESC derived from these two developmental sources might show differential gene expression profile. Comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

6 Samples

Download data: TXT

(Submitter supplied) To determine blastomere fate and embryonic genome activation (EGA) at 5- to 8-cell stage human embryos by global gene expression profile of amplified cDNA from blastomeres at the single cell level

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

53 Samples

Download data: TXT

(Submitter supplied) We present a compendium DNA microarray analysis of multiple mouse ESCs and EGCs from different genetic backgrounds (strains 129 and C57BL/6) cultured under standard conditions and in differentiation-promoting conditions by the withdrawal of Leukemia Inhibitory Factor (LIF) or treatment with retinoic acid (RA). All pluripotent cell lines showed similar gene expression patterns, which separated them clearly from other tissue stem cells with lower developmental potency. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL4358 GPL2552

50 Samples

Download data: TIFF, TXT

(Submitter supplied) In total 8,736,191 reads from IP fraction and 9,958,984 reads from Wash fraction uniquely mapped to the mouse genome (mm9). Settings for enrichment analysis: maxSpacing=500; minHits=20; minPeakHits=6; minRatio=3. 4,701 regions have been identified as enriched in the IP fraction. In order to get a rough idea of false discovery rate (FDR), enrichment analysis using Wash fraction as test and IP fraction as control was performed, 15 regions was found. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9185

1 Sample

Download data: TXT, XLS

(Submitter supplied) Polycomb group (PcG) proteins are highly conserved epigenetic transcriptional repressors important for the control of numerous developmental gene expression programs and have recently been implicated in the modulation of embryonic stem cell (ESC) identity. We identified the PcG protein PCL2 (polycomb-like 2) in a genome-wide screen for novel regulators of self-renewal and pluripotency and predicted that it would play an important role in mouse ESC fate determination. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) Pig induced pluripotent stem cells (piPSCs) have significant biomedical and agricultural applications. We analyzed the transcriptional profiles of pig iPSC lines derived from different labs using Affymetrix GeneChip Pig Genome Array and published microarray datasets of mouse and human iPSCs. Our results demonstrated that cell surface proteins of EpCAM (epithelial cells adhesion molecule) were significantly upregulated in complete fully reprogrammed pig iPSCs, but not in partially reprogrammed cells. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

6 Samples

Download data: CEL

(Submitter supplied) Induced pluripotent stem cells (iPSCs) outwardly appear to be indistinguishable from embryonic stem cells (ESCs). A study of gene expression profiles of mouse and human ESCs and iPSCs suggests that, while iPSCs are quite similar to their embryonic counterparts, a recurrent gene expression signature appears in iPSCs regardless of their origin or the method by which they were generated. Upon extended culture, hiPSCs adopt a gene expression profile more similar to hESCs; however, they still retain a gene expression signature unique from hESCs that extends to miRNA expression. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL8941 GPL570

36 Samples

Download data: CEL, GPR

(Submitter supplied) Mouse ESCs depleted of the epigenetic modifying enzyme Usp22 fail to differentiate properly. Ectopic expresison of Usp22 results in spontaneous differnetiation. In order to understand the transcriptional program underlying this biological defect, whole genome expression analysis was performed.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4973

Platform:

GPL6246

4 Samples

Download data: CEL

Analysis of E14 embryonic stem cells (ESCs) depleted for ubiquitin specific protease 22 (Usp22). ESCs depleted of the epigenetic modifying enzyme Usp22 fail to differentiate properly. Results provide insight into the role of Usp22 in ESC differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL6246

Series:

GSE42934

4 Samples

Download data: CEL

(Submitter supplied) The membrane fraction and the cytosolic fraction of HES2 cells were collected and subjected to microarray. The experiment was performed in triplicate Keywords: membrane-polysome fractionation of RNA

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6102

6 Samples

Download data: TXT

(Submitter supplied) In the following experiment, three different hESC cell lines (HES2, MEL1 and H9) were grown in the presence of KOSR, KOSR or mTESR containing media respectively. KOSR (Knockout serum replacement medium) is a standard media allowing the growth of hESC without the need for manual passaging - Enzymatic passaging is used instread. mTESR (Ludwig et al., 2007) is a media allowing the growth of hESC on matrigel with enzymatic passaging. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6102

36 Samples

Download data: TXT

(Submitter supplied) HES2 ESCs were grown in standard ES culture conditions. After 1 week, these cells were FACs sorted for the presence of GCTM-2 and CD9. Keywords: Sorted cell comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6102

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Betapolyomavirus macacae; Mus musculus; Human gammaherpesvirus 8; Rattus norvegicus; Homo sapiens; Macacine gammaherpesvirus 4

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL7088 GPL7089

38 Samples

Download data: GPR

(Submitter supplied) Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. We aimed to identify the unique signature of miRNAs in human embryonic stem cells. more...

Organism:

Rattus norvegicus; Macacine gammaherpesvirus 4; Homo sapiens; Mus musculus; Human gammaherpesvirus 8; Betapolyomavirus macacae

Type:

Non-coding RNA profiling by array

Platform:

GPL7089

21 Samples

Download data: GPR

(Submitter supplied) Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. However, much work needs to be done to understand the molecular mechanisms responsible for the maintenance of the undifferentiatiation state and the differentiation process of human embryonic stem cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7088

17 Samples

Download data: GPR