GEO DataSets

Analysis of H10 osteoclastogenic cells following treatment with soluble recombinant receptor activator of nuclear factor-kB ligand to induce osteoclastogenesis. H10 cells derived from RAW264.7 macrophages. Results provide insight into the molecular mechanisms regulating osteoclastogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 cell line sets

Platform:

GPL6246

Series:

GSE43811

6 Samples

Download data: CEL

(Submitter supplied) The primary aim of this project was to identify novel factors, in particular the cell-surface protein CD109, which regulate osteoclastogenesis. Microarray analysis was performed comparing two pre-osteoclast cell lines generated from the RAW 264.7 osteoclast cell line: one that has the capacity to fuse forming large multinucleated cells and one that does not fuse. It was found that CD109 was up-regulated by > 17-fold in the osteoclast forming cell line when compared to the cell line that does not fuse.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5398

Platform:

GPL6246

6 Samples

Download data: CEL

(Submitter supplied) Osteoclast (OC) differentiation undergoes a two-step process: commitment of hematopoietic progenitor cells to tartrate-resistant acid phosphatase (TRAcP) positive OC precursors (OCPs), and fusion of OCPs into multinucleated OCs. In order to identify transcriptional profiles of genes in the transitional phase between OC commitment and fusion in OCG, Affymetrix® Mouse Gene 1.0 ST arrays were performed on total RNA extracted from mouse (SV129/BL6 ) monocytes and pre-osteoclasts (pre-OCs), primed with macrophage colony-stimulated factor (M-CSF) or M-CSF and soluble recombinant receptor activator of NF-кB ligand (sRANKL), respectively. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

6 Samples

Download data: CEL, PNG, TXT

(Submitter supplied) Comparison of gene expression of the osteoclast precursor myeloid blast seeded on plastic and on bone, primed with M-CSF for 4 days and culture with M-CSF and RANKL for 1 day. Osteoclasts and macrophages share progenitors that must receive decisive lineage signals driving them into their respective differentiation routes. Macrophage colony stimulation factor M-CSF is a common factor; bone is likely the stimulus for osteoclast differentiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

1 Sample

Download data: TXT

(Submitter supplied) Effect of c-fos expression in osteoclastogenic splenocytes. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS766

Platform:

GPL81

5 Samples

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Analysis of the role of c-Fos and Nfat in osteoclastogenesis by expression profiling of osteoclastogenic splenocytes lacking c-Fos but with an active Nfatc4. Active Nfatc4 introduced into cells by retroviral gene transfer. Osteoclastogenesis restored by active Nfatc4 in splenocytes lacking c-Fos.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 2 genotype/variation, 3 protocol sets

Platform:

GPL81

Series:

GSE676

5 Samples

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