GEO DataSets

Temporal analysis of JIMT1 breast cancer cells cultured as xenografts, in two dimensional cultures, in polyHEMA three dimensional cell cultures, and in Matrigel three dimensional cell cultures. Results provide insight into how closely the Matrigel 3D cell culture model mimics in vivo tumors.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 4 growth protocol, 3 time sets

Platform:

GPL10558

Series:

GSE42529

12 Samples

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(Submitter supplied) The traditional method for studying cancer in vitro is to grow immortalized cancer cells in two-dimensional (2D) monolayers on plastic. However, many cellular features are impaired in these unnatural conditions and big alterations in gene expression in comparison to tumors have been reported. Three-dimensional (3D) cell culture models have become increasingly popular and are suggested to be better models than 2D monolayers due to improved cell-to-cell contacts and structures that resemble in vivo architecture. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

12 Samples

Download data: TXT

(Submitter supplied) The traditional method for studying cancer in vitro is to grow immortalized cancer cells in two-dimensional (2D) monolayers on plastic. However, many cellular features are impaired in these unnatural conditions and big alterations in gene expression in comparison to tumors have been reported. Three-dimensional (3D) cell culture models have become increasingly popular and are suggested to be better models than 2D monolayers due to improved cell-to-cell contacts and structures that resemble in vivo architecture. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5310

Platform:

GPL10558

12 Samples

Download data: TXT

(Submitter supplied) Comparison of gene expression data between cell lines grown on 2D tissue culture plastic or 3D matrigel A427, A549, H23, H358, H460, H661, H1437, and H1703 cells were compared

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

16 Samples

Download data: CEL

(Submitter supplied) Commonly used monolayer cell cultures lack the capacity to provide a physiologically relevant environment for cell culture in terms of cell-cell architecture, extracellular matrix composition, and spatiotemporal delivery of key growth factors and small molecules, such as oxygen. Here, we describe a three-dimensional (3D) approach to cell culture in vitro, utilizing a bioreactor system designed to control oxygenation of 3D cancer cell cultures, in order to better mimic tumor microenvironments observed in vivo. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

58 Samples

Download data: TXT

(Submitter supplied) Full title: Comprehensive Characterization of Three-Dimensional Models for Prostate Cancer Growth and Invasion in Laminin-rich Extracellular Matrix Prostate Cancer (PrCa) cells undergo acinar morphogenesis and spheroid formation in three-dimensional (3D) culture, supported by laminin-rich extracellular matrix (lrECM, Matrigel). We developed miniaturized 3D model systems that facilitate investigation of morphogenesis and invasion of normal and PrCa cell lines in lrECM. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

54 Samples

Download data: TXT

(Submitter supplied) Comparison of gene expression of different colon carcinoma cell lines under 2D and 3D culturing conditions

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL14550

49 Samples

Download data: TXT

(Submitter supplied) To demonstrate the versatility of parallel-factor ChIP-seq, we applied our method to the analysis of ER binding in five patient-derived xenograft (PDX) samples.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

10 Samples

Download data: NARROWPEAK