GEO DataSets

Analysis of MCF-7 cell line treated with chemotheraputic agent cisplatin and collected when glycolysis was induced (change of ph; 8-9h) and at the beginning of cell death (change of impedance; 10-11h). Results provide insight into molecular mechanisms underlying the specific metabolic response.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 metabolism, 3 time sets

Platform:

GPL570

Series:

GSE28274

6 Samples

Download data: CEL

(Submitter supplied) Treatment of MCF7 breast cancer cells by cisplatin leads to a very specific metabolic response and an onset of cell death about 10-11 h after beginning of treatment. For more detailed understanding of the molecular processes underlying the specific metabolic response, mRNA was isolated from MCF7 cells when the specific changes, (i) induction of glycolysis and (ii) onset of cell death, were detected during online measurement in the cell biosensor system.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3910

Platform:

GPL570

6 Samples

Download data: CEL

(Submitter supplied) Chemotherapeutic use of cisplatin is limited by its severe side effects. In this study, we demonstrated that cisplatin induces cell death in a proximal tubular cell line by suppressing glycolysis- and tricarboxylic acid (TCA)/mitochondria-related genes. HK-2 cells were cultured to confluence in 100mm dishes. Total RNA was extracted (QIAGEN, Valencia, CA, USA), and the concentration in the samples was measured using a Micro UV-Vis fluorescence spectrophotometer (Malcom, Tokyo, JAPAN). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

6 Samples

Download data: CEL, CHP