GEO DataSets

Analysis of cultured peripheral blood mononuclear cells (PBMCs) stimulated with interleukin-12 (IL-12). PBMCs obtained from 7 donors. IL-12 plays a central role in adaptive and innate immunity. Results provide further insight into the effects of IL-12 on the immune response.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 7 individual sets

Platform:

GPL97

Series:

GSE12839

14 Samples

Download data: CEL

(Submitter supplied) The cytokine interleukin-12 (IL-12) is known to play a central role in adaptive and innate immunity. We employed microarray analysis of IL-12 induced gene expression to provide further insights into its effects on immune response. Keywords: Paired study of expression levels: Treated vs. Control

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS3497 GDS3498

Platforms:

GPL97 GPL96

28 Samples

Download data: CEL

Analysis of cultured peripheral blood mononuclear cells (PBMCs) stimulated with interleukin-12 (IL-12). PBMCs obtained from 7 donors. IL-12 plays a central role in adaptive and innate immunity. Results provide further insight into the effects of IL-12 on the immune response.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 7 individual sets

Platform:

GPL96

Series:

GSE12839

14 Samples

Download data: CEL

(Submitter supplied) T-bet is a critical transcription factor for T helper 1 (Th1) cell differentiation. To study the regulation and functions of T-bet, we developed a T-bet-ZsGreen reporter mouse strain, in which GFP faithfully reflects the expression of T-bet. By using this tool, we report that signals elicited by IL-12 and IFNg are redundant in inducing T-bet in mice infected with Toxoplasma gondii and that T-bet does not contribute to its own expression when induced by IL-12 and IFNg. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL9250 GPL13112

9 Samples

Download data: XLS

(Submitter supplied) Follicular helper T (Tfh) cells comprise an important subset of helper T cells; however, their relationship with other helper lineages is incompletely understood. Herein, we show IL-12 acting via signal transducer and activator of transcription 4 (STAT4) induced both Il21 and Bcl6 genes, generating cells with features of both Tfh and Th1 cells. However, STAT4 also induced T-bet. Using ChIP-seq, we defined the genome-wide targets of T-bet and found that it repressed Bcl6 and other markers of Tfh cells, thereby attenuating the nascent Tfh-like phenotype in the late phase of Th1 specification. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11002

9 Samples

Download data: BEDGRAPH

(Submitter supplied) IL-27 is a potent antagonist of TH1-mediated inflammation, but the basis for this effect is not fully understood. Recent studies identified a population of T-bet+ CXCR3+ Treg that limit TH1-mediated immune pathology. The studies presented here demonstrate that IL-27-mediated STAT1 activation promotes Treg expression of T-bet and CXCR3. Infection with Toxoplasma gondii induced a similar Treg population that limits T cell responses and this population at mucosal sites is IL-27-dependent. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

18 Samples

Download data: TXT

(Submitter supplied) In autoimmune diseases, accumulation of activated leukocytes correlates with inflammation and disease progression, and therefore, disruption of leukocyte trafficking is an active area of research. The protein kinase Tpl2 (MAP3K8) regulates leukocyte inflammatory responses and is also being investigated for therapeutic inhibition during autoimmunity. Herein, we addressed the contribution of Tpl2 to the regulation of macrophage chemokine and chemokine receptor expression and subsequent migration in vivo using a mouse model of Tpl2 ablation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5385

Platform:

GPL1261

8 Samples

Download data: CEL

Analysis of tumor progression locus 2 (Tpl2)-deficient bone marrow derived macrophages (BMDMs) stimulated with lipopolysaccharide (LPS) for 4 hrs. Tpl2 (Map3k8) is a serine/threonine kinase critical in innate immunity. Results provide insight into the role of Tpl2 in BMDMs during acute inflammation.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL1261

Series:

GSE48338

8 Samples

Download data: CEL

(Submitter supplied) We perform CRISPR knockout of IRAK4 usig two different sgRNAs in murine KP2 cells, and then reexpressed murine IRAK4 in these two KO cell lines. We performed RNAseq on wild-type, IRAK4 KO and IRAK4 KO/rescue cell lines to investigate pathways contolled by IRAK4.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: TXT

(Submitter supplied) Host defense requires the specification of CD4+ helper T (Th) cells into distinct fates including Th1 cells that preferentially produce interferon γ(IFN-γ ).IFN-γ , a member of a large family of anti-pathogenic and anti-tumor IFNs, induces T-bet, a lineage defining transcription factor for Th1 cells, which in turn supports IFN-γ production in a feed-forward manner. Herein, we show a cell intrinsic role of T- bet to influence how T cells perceive their secreted product in the environment. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL13112 GPL17021

110 Samples

Download data: BED, BW, TXT

(Submitter supplied) Severe fever with thrombocytopenia syndrome phlebovirus (SFTSV), listed in the WHO most dangerous pathogens, has 12-30% fatality rates with a characteristic thrombocytopenia syndrome. With a majority of clinically diagnosed SFTSV patients older than ~50 years, age is a critical risk factor for SFTSV morbidity and mortality. Here, we report an age-dependent ferret model of SFTSV infection and pathogenesis that fully recapitulates the clinical manifestations of human infections. more...

Organism:

Mustela putorius furo

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25735

13 Samples

Download data: XLS, XLSX

(Submitter supplied) Bone marrow derived macrophages (BMDM) generated from c57bl/6j mice bone marrow cells were stimulated for 18 h with 12 microgram/ml adiponectin, RNA from non-stimulated or 18 h adiponectin-stimulated BMDM subjected to a agilent microarray analysis

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

3 Samples

Download data: TXT

(Submitter supplied) TPL2 (MAP3K8) is a central signaling node in the inflammatory response of peripheral immune cells.  We find that TPL2 kinase activity modulates microglial cytokine release and is required for microglia-mediated neuron death in vitro.  In acute in vivo neuroinflammation settings, TPL2 kinase activity regulates cytokine levels and activation states of microglia.  In a tauopathy model of chronic neurodegeneration, loss of TPL2 kinase activity reduces neuroinflammation and rescues synapse loss, brain volume loss, and behavioral deficits.  Single-cell RNAseq analysis indicates protection in the tauopathy model was associated with reductions in activated microglia subpopulations as well as infiltrating peripheral immune cells.  Overall, using various models, we find that TPL2 kinase activity promotes multiple harmful consequences of microglial activation in the brain including cytokine release, iNOS induction, astrocyte activation, and immune cell infiltration.  Consequently, inhibiting TPL2 kinase activity could represent a potential therapeutic strategy in neurodegenerative conditions.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

18 Samples

Download data: RDATA

(Submitter supplied) Identification and comparison of genes induced by IRF1 ectopic expression versus IFNγ treatment in A549 lung epithelial fibroblasts.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

9 Samples

Download data: TXT, XLSX

(Submitter supplied) IFN-g, critical for host defense and tumor surveillance, requires tight control of its expression. Multiple cis-regulatory elements exist around Ifng along with a non-coding transcript, Ifng-as1 (also termed NeST). Here, we describe two genetic models generated to dissect the molecular functions of this locus and its RNA product. DNA Deletion within the Ifng-as1 locus disrupted chromatin organization of the extended Ifng locus, impaired IFN-g response and compromised host defense. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:

GPL17021 GPL21493

59 Samples

Download data: BW, HIC

(Submitter supplied) The hormonal milieu influences immune tolerance as well as the immune response against viruses and cancer, but the direct effect of androgens on cellular immunity remains largely uncharacterized. We therefore sought to evaluate the effect of androgens on murine CD4 T-cells by micro-array analysis

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5301

Platform:

GPL11180

4 Samples

Download data: CEL

Analysis of CD4 T cells from castrated animals. Testosterone levels had been observed to be inversely correlated to the strength of the immune response. Results provide insight into the molecular basis of this observation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL11180

Series:

GSE54945

4 Samples

Download data: CEL