GEO DataSets

Analysis of peripheral blood-derived monocytes at various time points up to 24 hours following treatment with lipopolysaccharide. Results compared with those obtained from Massively Parallel Signature Sequencing.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 5 time sets

Platform:

GPL570

Series:

GSE5504

10 Samples

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(Submitter supplied) To evaluate gene expression in human peripheral blood derived monocytes over the course of an LPS stimulation time-series. Keywords: time course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2856

Platform:

GPL570

10 Samples

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(Submitter supplied) A comparison of microarray and MPSS technologies can help to establish the metrics for data comparisons across these technology platforms and determine some of the factors affecting the measurement of mRNA abundances using different platforms. Here, different Treatments/Conditions based on different Arabidopsis tissues were used for three different platforms include MPSS, Affymetrix and Agilent. Keywords: MPSS, Affymetrix, Agilent

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platforms:

GPL888 GPL5800 GPL198

44 Samples

Download data: CEL, TIFF, TXT

(Submitter supplied) Comparison of gene expression in wildtype and MyD88-/- C57BL/6J mouse macrophages treated with 10 ng/mL LPS for 2 hours versus media treated control macrophages, and, wildtype and MyD88-/- C57BL/6J mouse macrophages treated with live E. coli bacteria (log phase; 1 bact per 1 macrophage) for 2 hours versus media treated control macrophages. Cells from 4 mice of each geneotype were used and each individual provided its own control. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS1705 GDS1706

Platform:

GPL1226

31 Samples

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Analysis of MyD88 null mutant macrophages treated with LPS or live E. coli. MyD88 transduces cell signaling events downstream of Toll-like receptors, a key component of host defense. Results suggest most of the host response to endotoxin or live bacteria is actually regulated independently of MyD88.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 agent, 2 genotype/variation sets

Platform:

GPL1226

Series:

GSE1405

15 Samples

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Analysis of MyD88 null mutant macrophages treated with LPS or live E. coli. MyD88 transduces cell signaling events downstream of Toll-like receptors, a key component of host defense. Results suggest most of the host response to endotoxin or live bacteria is actually regulated independently of MyD88.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 agent, 2 genotype/variation sets

Platform:

GPL1226

Series:

GSE1405

16 Samples

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(Submitter supplied) Since macrophage activation can now be studied at a global level using modern microarray and proteomic analyses, discovery of novel macrophage activation genes is inevitable and important for understanding HIV-associated dementia (HAD). We isolated two different types of primary human macrophages, microglia and monocyte-derived macrophages (MDM) from brain tissue and whole blood respectively. The microarray analysis of differentially regulated macrophage activation genes reported here supports our previous assertions that the mixed glia (MIX) cultured in starvation conditions (DMEM alone) are a non-activated, or “quiescent”, tissue culture model for studying macrophage activation in the brain. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

14 Samples

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(Submitter supplied) We used the MPSS technology to uncover gene expression profiling in a greater depth in the early embryonic gonads and primordial germ cells (PGCs) in the chicken. Total numbers of sequenced signatures were 1,012,533 and 995,676 for the PGCs and gonad. Using a false discovery rate cut-off of 0.05, we found 3.05 % of all signatures in the PCGs and 1.89 % of all signatures in the gonad signatures were significantly up-regulated compared to each sample. more...

Organism:

Gallus gallus

Type:

Expression profiling by MPSS

Platform:

GPL4337

2 Samples

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(Submitter supplied) Neonatal gram-negative sepsis is often characterized by a fulminat clinical course compared to adults resulting in higher morbidity and mortality. Genome-wide gene expression analysis can provide insights into the molecular alterations in sepsis. To evaluate in vitro activation of the neonatal and adult immune system, gene expression patterns were compared in mononuclear cells from cord (CBMNC) and adult peripheral blood (APBMNC). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1310

Platform:

GPL201

12 Samples

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Analysis of neonatal mononuclear cells from cord blood incubated with 5 ug/ml lipopolysaccharide (LPS). Effect of LPS on adult mononuclear cells from peripheral blood compared. Results provide insight into the molecular basis for increased susceptibility of neonates to sepsis.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 2 cell type sets

Platform:

GPL201

Series:

GSE3140

12 Samples

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(Submitter supplied) Paeony root has long been used for its anti-inflammatory effects. In this study, the effects of albiflorin, paeoniflorin, and paeonol, compounds from paeony root, on gene expression profiles were examined in macrophages. The RAW264.7 macrophages were treated with LPS in the presence or absence of albiflorin, paeoniflorin, or paeonol. Global mRNA expression levels were detected by using an oligonucleotide microarray platform covering the mouse whole genome. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

33 Samples

Download data: TXT

(Submitter supplied) Bovine tuberculosis (BTB) caused by Mycobacterium bovis continues to cause substantial losses to global agriculture and has significant repercussions for human health. The advent of high throughput genomics has facilitated large scale gene expression analyses that present a novel opportunity for revealing the molecular mechanisms underlying chronic mycobacterial infection. Using this approach, we have previously shown that innate immune genes in peripheral blood mononuclear cells (PBMC) from BTB-infected animals are repressed in vivo in the absence of exogenous stimulation. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL5751

36 Samples

Download data: GPR