GEO DataSets

(Submitter supplied) N6-methyladenosine (m6A) is the most prominent mRNA modification in eukaryotes, and its potential regulatory role has recently been identified in mammals, plants, and yeast. However, how m6A methylation regulates spermatogenesis remains unknown. In this study, cattle-yak testis tissue was used as the experimental material, and the m6A map was generated through preliminary experiments and methylated RNA immunoprecipitation sequencing. more...

Organism:

Bos grunniens

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL30466

6 Samples

Download data: TXT

(Submitter supplied) N6-methyladenosine (m6A) is the most common internal modification of eukaryotic mRNA, and its potential regulatory role has recently been identified in mammals, plants, and yeast. However, little is known about the role of m6A methylation in mammalian sexual maturation. In this study, the testicular tissue of yaks before and after sexual maturation was taken as the research object, and the m6A map of testicular tissue of yaks before and after sexual maturation was obtained through the preliminary experiment and MERIP-seq. more...

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL30466

12 Samples

Download data: TXT

(Submitter supplied) Testis is the most important male reproductive organ, and the integrity of its physiological function is crucial to the successful production of sperm. In this study, the expression profiles of 11 991 and 8 930 cells in testicular tissue of yak and cattle-yak after sexual maturity were established using Single-cell RNA sequencing. The identification results of cell subpopulations and marker genes were analyzed and their possible mechanisms were predicted.

Organism:

Bos grunniens x Bos taurus; Bos grunniens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL30409 GPL32644

2 Samples

Download data: MTX, TSV

(Submitter supplied) RNA sequence analysis identified a total of 8473 differentially expressed genes (DEGs) (3580 up-regulated, 4893 down-regulated), and GO annotation and KEGG enrichment of differential genes found that DEGS were mostly related to spermatogenesis and apoptosis. Among them, Spermatogonia stem cell (SSCs) marker genes (ITGA5, Gfra1, CD9, SOHLH1, SALL4, ID4 and FOXO1) were significantly up-regulated, differentiation spermatogenic cell marker genes (KIT, UCHL1, Ccna1, TNP1 and TXNDC2) were significantly down-regulated, and genes involved in apoptosis (Fas, CTSK, CTSB, CTSC, CTSO, caspase 3, caspase6, and caspase) were significantly up-regulated. more...

Organism:

Bos grunniens; Bos grunniens x Bos taurus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32499 GPL22950

6 Samples

Download data: TXT

(Submitter supplied) The Jeryak is the hybrid offspring of yaks and Jersey cattle and exhibit improved milk and meat yields. Biomolecules carried within milk exosomes are important for cell growth, development, immune regulation, and various pathophysiological processes. Previous studies showed that miRNAs regulate mammary gland development, lactation, and milk quality. This study explored the relationship between milk exosomes miRNAs and lactation performance. more...

Organism:

Bos grunniens; Bos grunniens x Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL30409 GPL32644

12 Samples

Download data: XLSX

(Submitter supplied) We ascertain that mRNA m6A methylation exhibits declined change during bovine skeletal myoblast myogenic differentiation. RNA-seq and m6A-seq (MeRIP-seq) analysis reveal that m6A methylation is an abundant modification in the mRNA of bovine myoblast and myotubes, and the m6A sites are mainly enriched near the stop codons and the 3′ untranslated regions.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platform:

GPL26012

12 Samples

Download data: TXT