GEO DataSets

(Submitter supplied) The aim of the present study was to explore commonly and preferentially expressed genes for male and female gamete development in synthetic Brassica allohexaploids. We performed differential expression analysis to identify precise genes that regulate developmental events during male and female gamete development of Brassica allohexaploids.

Organism:

Brassica carinata x Brassica rapa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32203

9 Samples

Download data: XLS

(Submitter supplied) We explored the transcriptomic changes of synthetic Brassica allohexaploid by comparing to its parents using a high-throughput RNA-Seq method. A total of 35644409 sequence reads were generated, and 32642 genes were aligned from the data. There were 29260, 29060 and 29697 genes identified in Brassica rapa, Brassica carinata, and Brassica allohexaploid, respectively. We screened differentially expressed genes (DEGs) by a standard of two-fold or greater change in expression and false discovery rate (FDR) no more than 0.001. more...

Organism:

Brassica rapa; Brassica carinata x Brassica rapa; Brassica carinata

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL17053 GPL16443 GPL16440

3 Samples

Download data: TXT

(Submitter supplied) We studied the causes of sterility underlying multiple-allele-inherited male sterility in Chinese cabbage by identifying differentially expressed genes (DEGs) related to pollen sterility between fertile and sterile flower buds. In this work,we performed transcriptome analysis of mRNA isolated from fertile and sterile buds using Illumina HiSeq 2000 platform sequencing. Approximately 80% of ~229 million high quality paired-end reads were uniquely mapped to the reference genome. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17788

6 Samples

Download data: XLS

(Submitter supplied) The gynoecium is one of the most complex organs of angiosperms specialized for seed production and dispersal, but only several genes important for ovule or embryo sac development were identified by using female sterile mutants. The female sterility in oilseed rape (Brassica napus) was before found to be related with one alien chromosome from another crucifer Orychophragmus violaceus. Herein, the developmental anatomy and comparative transcript profiling (RNA-seq) for the female sterility were performed to reveal the genes and possible metabolic pathways behind the formation of the damaged gynoecium. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

2 Samples

Download data: TXT

(Submitter supplied) Analysis of the different gene expression profiles of natural and resynthesized Brassica polyploids with Illumina deep sequencing technology could help to improve our knowledge of polyploid genome evolution. We obtained approximately 6 million sequence tags per sample,and 6018254, 5930726, 6022170, 5950123, 5991210, 5798939, 5823113, 5772449,5858527 and 5657697 clean tags were obtained in libraries of B. more...

Organism:

Brassica rapa; Brassica carinata; Brassica oleracea; Brassica juncea; Brassica napus; Brassica nigra

Type:

Expression profiling by high throughput sequencing

6 related Platforms

10 Samples

Download data: TXT

(Submitter supplied) Mutation of the ASHH2 gene has pleiotropic developmental effects. We have focused on the role of ASHH2 in plant reproduction.The aim of the study was to identify changes in gene expression in inflorescences of the ashh2-1/sdg8-1 mutant (SALK_065480, insertion in the gene At1g77300, SDG8/EFS/ASHH2) compared to wt Arabidopsis thaliana (ecotype Colombia) inflorescences. On the female side, close to 80% of mature ovules lack embryo sac. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL9125

5 Samples

Download data: GPR

(Submitter supplied) Anther development is a complex process, and the study of its molecular mechanism has an important impact on plant breeding. This study aims to identify microRNA (miRNA), mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) related to anther development of Chinese cabbage, so as to construct competitive endogenous RNA (ceRNA) regulatory networks and provide valuable knowledge for the exploration of pollen development mechanism of Chinese cabbage. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24709

2 Samples

Download data: TXT

(Submitter supplied) Anther development is a complex process, and the study of its molecular mechanism has an important impact on plant breeding. This study aims to identify microRNA (miRNA), mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) related to anther development of Chinese cabbage, so as to construct competitive endogenous RNA (ceRNA) regulatory networks and provide valuable knowledge for the exploration of pollen development mechanism of Chinese cabbage. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29989

2 Samples

Download data: TXT

(Submitter supplied) This study compared the transcriptomes of maize anthers at both 1mm and 1.5mm lengths from male sterile mutants with either the am1-489 or am1-pra alleles as well as from fertile siblings of the mutant plants. Comparisons were done varying just the stage (1mm vs 1.5 mm), just the allele (am1-pra vs am1-489 at the same stage), and wild-type vs mutant (same stage and allele). Genes were categorized as On (expressed) or Off (not detected) and if both channels produced an intensity, the differential expression was determined. more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL7444

64 Samples

Download data: TXT

(Submitter supplied) In flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. more...

Organism:

Oryza sativa

Type:

Expression profiling by array

Platform:

GPL6864

33 Samples

Download data: TXT

(Submitter supplied) We present an atlas of global gene expression covering embryo and seed coat development in B. rapa, B. nigra, B. oleracea, B. juncea, B. napus and B. carinata, providing insights into the evolution of gene expression in embryogenesis and seed development of brassica species.

Organism:

Brassica juncea; Brassica rapa; Brassica napus; Brassica nigra; Brassica carinata; Brassica oleracea

Type:

Expression profiling by high throughput sequencing

6 related Platforms

270 Samples

Download data: XLSX

(Submitter supplied) Global gene expression profiles of seven stages representing 29 days of anther development are analyzed using a 44K oligonucleotide array querying ~80% of maize protein-coding genes. Each anther stage expresses ~10,000 constitutive and ~10,000 or more transcripts restricted to one or a few stages. Keywords: anther development, maize

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL7209

28 Samples

Download data: TXT

(Submitter supplied) Seedlessness is a highly relevant trait in grapevine berries that are intended for fresh consumption or raisin production. Corinto Bianco (CB) is a parthenocarpic somatic variant of the seeded cultivar Pedro Ximenes (PX). Both variants were compared to understand the basis of this parthenocarpic phenotype. CB berries ripened normally although their size was six times smaller than in PX. Macrogametogenesis was aborted in CB, which was also pollen sterile. more...

Organism:

Vitis vinifera

Type:

Expression profiling by array

Platform:

GPL17894

6 Samples

Download data: PAIR

(Submitter supplied) Transcriptomes from multiple pre-meiotic stages of wild type, mac1, and msca1 maize anthers were characterized by microarray hybridization. The goal was to characterize the developmental progression as the anther specifies five cell types and grows rapidly precedeing meiotic entry. The stages characterized were immature anther primordia (0.15 mm long in maize) containing just stem cells, through somatic and germinal cell fate specification (0.20 and 0.25 mm), mitotic proliferation (0.4 mm), and finally the birth of the middle layer and tapetum (0.7 mm). more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL7444

48 Samples

Download data: TXT

(Submitter supplied) Background: Partial pollen and embryo sac sterilities are the two main reasons for low fertility in autotetraploid rice. Our previous study revealed that small RNAs changes may associate with pollen fertility in autotetraploid rice. However, knowledge on comparative analysis between the development of pollen and embryo sac by small RNAs in autotetraploid rice is still unknown. In the present study, WE-CLSM (whole-mount eosin B-staining confocal laser scanning microscopy) and high-throughput sequencing technology was employed to examine the cytological variations and to analyze small RNAs changes during pollen and embryo sac development in autotetraploid rice compared with its diploid counterpart. more...

Organism:

Oryza sativa Japonica Group

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18525

16 Samples

Download data: XLSX

(Submitter supplied) Transcriptional profiling of mitotic archesporial cells, early pollen mother cells and pollen mother cells comparing control tapetal cells, pollen, parenchyma cells and seedling. Goal was explain the mitosis/meiosis transition at the molecular level.

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL20611

20 Samples

Download data: TXT